A5100759007- Plant Molecular Biology Research
- Plant Reproductive Biology
- RNA modifications and cancer
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Tea Polyphenols and Effects
- RNA Research and Splicing
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Phytochemicals and Antioxidant Activities
- Biofuel production and bioconversion
- Ginseng Biological Effects and Applications
- Plant Disease Resistance and Genetics
- Single-cell and spatial transcriptomics
- Plant Physiology and Cultivation Studies
- Genomics and Chromatin Dynamics
- Horticultural and Viticultural Research
- Plant and Fungal Interactions Research
- Plant responses to water stress
- Extracellular vesicles in disease
Fujian Agriculture and Forestry University
2018-2025
Wuhan University of Science and Technology
2023-2025
Broad Institute
2021-2025
Ministry of Agriculture and Rural Affairs
2023-2024
Hubei Academy of Agricultural Sciences
2023-2024
Zhejiang Sci-Tech University
2024
Second Hospital of Tianjin Medical University
2024
Tianjin Medical University
2024
University of Technology
2023
ZheJiang Academy of Agricultural Sciences
2023
Abstract The recently identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of COVID-19 pandemic. How this novel beta-coronavirus virus, and coronaviruses more generally, alter cellular metabolism to support massive production ~30 kB viral genomes subgenomic RNAs remains largely unknown. To gain insights, transcriptional metabolomic analyses are performed 8 hours after SARS-CoV-2 infection, an early timepoint where lifecycle completed but prior overt effects on...
Oolong tea is a popular and semi-fermented beverage. During the processing of leaves, withering first indispensable process for improving flavor. However, roles long non-coding RNAs (lncRNAs) characteristic secondary metabolites during oolong leaves remain unknown. In this study, phytochemical analyses indicated that total polyphenols, flavonoids, catechins (EGC, CG, GCG, ECG, EGCG) were all less abundant in solar-withered (SW) than fresh (FL) indoor-withered (IW). contrast, terpenoid, JA,...
Banana (Musa spp.), one of the most important fruits worldwide, is generally cold sensitive. In this study, by using cold-sensitive banana variety Tianbaojiao acuminate) as study material, we investigated effects Piriformospora indica on resistance. Seedlings with and without fungus colonization were subjected to 4 °C treatment. The changes in plant phenotypes, some physiological biochemical parameters, chlorophyll fluorescence expression eight cold-responsive genes leaves before after...
Abstract The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune cellular responses associated with COVID-19 infection, symptoms, lethality. To address this, we collected tissues from 11 organs during clinical autopsy of 17 individuals who succumbed COVID-19, a bank approximately 420 specimens. We generated...
Abstract Background Albuminuria is a hallmark of diabetic kidney disease (DKD) that promotes its progression, leading to renal fibrosis. Renal macrophage function complex and influenced by metabolic status. However, the state macrophages impact albuminuria on are poorly understood. Methods Extracellular vesicles (EVs) from tubular epithelial cells (HK-2) were evaluated using transmission electron microscopy, nanoparticle tracking analysis western blotting. Glycolytic enzyme expression in...
The epitranscriptomic mark N
The unique aroma and flavor of oolong tea develop during the withering stage postharvest processing. We explored roles miRNA-related regulatory networks their effects on quality. conducted transcriptome miRNA analyses to identify differentially expressed (DE) miRNAs target genes among fresh leaves, indoor-withered solar-withered leaves. identified 32 DE-miRNAs 41 involved in phytohormone signal transduction ABC transporters. Further indicated that these two pathways regulated accumulation...
The two original plants of the oolong tea cultivar ('Tieguanyin') are "Wei shuo" 'Tieguanyin'—TGY (Wei) and "Wang (Wang). Another cultivar, 'Benshan' (BS), is similar to TGY in its aroma, taste, genetic make-up, but it lacks "Yin Rhyme" flavor. We aimed identify differences biochemical characteristics gene expression among these plants. results spectrophotometric, high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS) analyses revealed that (Wang) had...
Radish is an economically important root vegetable worldwide. In this study, the 217 cultivated radish accessions were collected and genotyped. To detect genotypes of these accessions, a total 24 structure variation (SV) markers distributed on nine chromosomes employed to analyze genetic diversity construct core germplasm collection radish. The results polymorphism information content (PIC) indicated good SV markers. Population analysis principal component (PCA) showed that fell into three...
Plant embryogenic calli (ECs) can undergo somatic embryogenesis to regenerate plants. This process is mediated by regulatory factors, such as transcription factors and specifically expressed genes, but the precise molecular mechanisms underlying at single-cell level remain unclear. In this study, we performed high-resolution RNA sequencing analysis determine cellular changes in EC of woody plant species Dimocarpus longan (longan) clarify continuous cell differentiation trajectories...
Abstract Background The molecular underpinnings of organ dysfunction in severe COVID-19 and its potential long-term sequelae are under intense investigation. To shed light on these the context liver function, we perform single-nucleus RNA-seq spatial transcriptomic profiling livers from 17 decedents. Results We identify hepatocytes positive for SARS-CoV-2 RNA with an expression phenotype resembling infected lung epithelial cells, a central role pro-fibrotic TGFβ signaling cell–cell...
Abstract Heat stress can seriously affect plant growth and development. Ethylene response factors (ERFs) play important roles in development physiological responses. Here, we identified DlERF6, an ERF family transcription factor that promotes heat tolerance Dimocarpus longan. DlERF6 was strongly induced by IAA treatment longan roots. Overexpression of generated abundant, fast-growing hairy roots enhanced promoting biosynthesis reactive oxygen species (ROS) scavenging. Additional assays...
DNA methylation is a highly conserved epigenetic modification involved in many biological processes, including growth and development, stress response, secondary metabolism. In the plant kingdom, cytosine-5 methyltransferase (C5-MTase) demethylase (dMTase) genes have been identified some species. However, to best of our knowledge, no investigator has focused on identification analysis C5-MTase dMTase tea plants (Camellia sinensis) based genome-wide levels. this study, eight CsC5-MTases four...
The APETALA2/ethylene responsive factor (AP2/ERF) superfamily members are transcription factors that regulate diverse developmental processes and stress responses in plants. They have been identified many However, little is known about the AP2/ERF longan (Dimocarpus Lour.), which an important tropical/subtropical evergreen fruit tree produces a variety of bioactive compounds with rich nutritional medicinal value. We conducted genome-wide analysis its roles somatic embryogenesis (SE) longan.A...
N6-methyladenosine (m6A), one of the internal modifications RNA molecules, can directly influence abundance and function without altering nucleotide sequence, plays a pivotal role in response to diverse environmental stresses. The precise m6A regulatory mechanism comprises three types components, namely, writers, erasers, readers. To date, research focusing on genes plant kingdom is still its infancy. Here, total 34 were identified from chromosome-scale genome tea plants. expansion was...
GRAS, named after GAI, RGA, and SCR, is a class of plant-specific transcription factors family that plays crucial role in growth development, signal transduction, various stress responses. To understand the biological functions banana GRAS gene family, genome-wide identification bioinformatics analysis was performed based on information from M. acuminata, balbisiana, itinerans genomic databases. In present study, we identified 73 MaGRAS, 59 MbGRAS, 58 MiGRAS genes bananas at whole-genome...
Abstract Alternative splicing (AS) is critically associated with tumorigenesis and patient's prognosis. Here, we systematically analyzed survival‐associated AS signatures in oral squamous cell carcinoma (OSCC) evaluated their prognostic predictive values. Survival‐related events were identified by univariate multivariate Cox regression analyses using OSCC data from the TCGA head neck set. The Percent Spliced In calculated SpliceSeq 0 to 1 was used quantify seven types of events. A model...
The molecular underpinnings of organ dysfunction in acute COVID-19 and its potential long-term sequelae are under intense investigation. To shed light on these the context liver function, we performed single-nucleus RNA-seq spatial transcriptomic profiling livers from 17 decedents. We identified hepatocytes positive for SARS-CoV-2 RNA with an expression phenotype resembling infected lung epithelial cells. Integrated analysis comparisons healthy controls revealed extensive changes cellular...
Abstract 2,3-Butanediol (2,3-BDO) is an important gateway molecule for many chemical derivatives. Currently, microbial production gradually being recognized as a green and sustainable alternative to petrochemical synthesis, but the titer, yield, productivity of 2,3-BDO remain suboptimal. Here, we used systemic metabolic engineering strategies debottleneck in Enterobacter aerogenes . Firstly, pyruvate network was reconstructed by deleting genes by-product synthesis improve flux toward which...
The plant-specific TCP transcription factors are well-characterized in both monocots and dicots, which have been implicated multiple aspects of plant biological processes such as leaf morphogenesis senescence, lateral branching, flower development hormone crosstalk. However, no systematic analysis the petunia gene family has described. In this work, a total 66 genes (32 PaTCP P. axillaris 34 PiTCP inflata) were identified. Subsequently, 32 was performed. phylogenetic combined with structural...
Circular RNAs (circRNAs) are widely involved in plant growth and development. However, the function of circRNAs somatic embryogenesis (SE) remains elusive. Here, by using high-throughput sequencing, a total 5029 were identified three stages longan (Dimocarpus Lour.) early SE. Kyoto Encyclopedia Genes Genomes (KEGG) analysis revealed that differentially expressed (DE) circRNA host genes enriched 'non-homologous end-joining' (NHEJ) 'butanoate metabolism' pathways. In addition, reactive oxygen...
Longan (Dimocarpus longan Lour.) is an economically important subtropical fruit tree. Its quality and yield are affected by embryo development. As a plant seed germination marker gene, the germin-like protein (GLP) gene plays role in However, mechanism underlying of GLP somatic embryos still unclear. Therefore, we conducted genome-wide identification (DlGLP) preliminarily verified function DlGLP1-5-1. Thirty-five genes were identified as divided into 8 subfamilies. Based on transcriptome...