A5003559322- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Skin and Cellular Biology Research
- RNA regulation and disease
- Genomics and Phylogenetic Studies
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pulmonary Hypertension Research and Treatments
- Single-cell and spatial transcriptomics
- Wnt/β-catenin signaling in development and cancer
- Aortic aneurysm repair treatments
- Immunodeficiency and Autoimmune Disorders
- Atherosclerosis and Cardiovascular Diseases
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Aortic Disease and Treatment Approaches
- Dermatological and Skeletal Disorders
- Epigenetics and DNA Methylation
- Blood disorders and treatments
- Diagnosis and treatment of tuberculosis
- interferon and immune responses
- Biochemical and Molecular Research
- Cancer-related gene regulation
- Leech Biology and Applications
Brigham and Women's Hospital
2020-2025
Broad Institute
2019-2025
Institute of Genomics and Integrative Biology
2013-2024
Academy of Scientific and Innovative Research
2014-2024
Council of Scientific and Industrial Research
2017-2024
Merck & Co., Inc., Rahway, NJ, USA (United States)
2024
Harvard University
2019-2022
Massachusetts General Hospital
2019-2020
Massachusetts Institute of Technology
2020
Government Medical College
2019
Background: The cells that form the arterial wall contribute to multiple vascular diseases. extent of cellular heterogeneity within these populations has not been fully characterized. Recent advances in single-cell RNA-sequencing make it possible identify and characterize subpopulations. Methods: We validate a method for generating droplet-based atlas gene expression normal blood vessel. Enzymatic dissociation 4 whole mouse aortas was followed by sequencing >10 000 cells. Results:...
Long non-coding RNAs (lncRNAs) are emerging as key regulators of endothelial cell function. Here, we investigated the role a novel vascular endothelial-associated lncRNA (VEAL2) in regulating permeability. Precise editing veal2 loci zebrafish (veal2gib005Δ8/+ ) induced cranial hemorrhage. In vitro and vivo studies revealed that competes with diacylglycerol for interaction protein kinase C beta-b (Prkcbb) regulates its activity. Using PRKCB2 bait, identified functional ortholog humans from...
Abstract The SARS-CoV-2 pandemic has caused over 1 million deaths globally, mostly due to acute lung injury and respiratory distress syndrome, or direct complications resulting in multiple-organ failures. Little is known about the host tissue immune cellular responses associated with COVID-19 infection, symptoms, lethality. To address this, we collected tissues from 11 organs during clinical autopsy of 17 individuals who succumbed COVID-19, a bank approximately 420 specimens. We generated...
The tubercle complex consists of closely related mycobacterium species which appear to be variants a single species. Comparative genome analysis different strains could provide useful clues and insights into the genetic diversity We integrated assemblies 96 from Mycobacterium tuberculosis (MTBC), included 8 Indian clinical isolates sequenced assembled in this study, understand its pangenome architecture. predicted genes for all clustered their respective CDSs homologous gene clusters (HGCs)...
This study systematically aims to evaluate the salivary microbiome in patients with primary Sjögren's syndrome (pSS) using 16S rRNA sequencing approach.DNA isolation and was performed on saliva of 37 pSS 35 control (CC) samples HiSeq 2500 platform. sequence analysis independently two popular computational pipelines, QIIME less operational taxonomic units scripts (LoTuS).There were no significant changes alpha diversity between controls. However, four genera including Bifidobacterium,...
Turner syndrome is a chromosomal abnormality characterized by the absence of whole or part X chromosome in females. This aneuploidy condition associated with diverse set clinical phenotypes such as gonadal dysfunction, short stature, osteoporosis and Type II diabetes mellitus, among others. These differ their severity penetrance affected individuals. Haploinsufficiency for few linked genes has been some these disease phenotypes. RNA sequencing can provide valuable insights to understand...
Liver plays a key role in maintaining glucose homeostasis and impaired hepatic metabolism is associated with type 2 diabetes. In the present study, we used RNA sequencing to profile transcriptome of livers diabetic db/db mice as compared normal db/+ identified 218 differentially expressed genes. Amongst these, there were 3 lncRNAs that significantly downregulated H19 was most altered lncRNA mice. expression correlated genes glycolysis gluconeogenesis pathways, which suggest levels can...
Asian seabass or barramundi (Lates calcarifer) is an important food fish with commercial value and a wide geographic distribution. Though some reports based on molecular and/or morphological data exist, comprehensive effort to establish species identity across its range lacking. In order address this issue especially ascertain whether the wide-spread distribution has resulted in bifurcation of species, we collected samples from various locations representing Western Eastern Coastline India,...
Earthworms show a wide spectrum of regenerative potential with certain species like Eisenia fetida capable regenerating more than two-thirds their body while other closely related species, such as Paranais litoralis seem to have lost this ability. belong the phylum Annelida, in which genomes marine oligochaete Capitella telata and freshwater leech Helobdella robusta been sequenced studied. Herein, we report transcriptomic changes (Indian isolate) during regeneration. Following injury, E....
Fomites are a well-known source of microbial infections and previous studies have provided insights into the sojourning microbiome fomites from various sources. Paper currency notes one most commonly exchanged objects its potential to transmit pathogenic organisms has been well recognized. Approaches identify associated with paper largely limited culture dependent approaches. Subsequent portrayed use 16S ribosomal RNA based approaches which taxonomical distribution microbiome. However,...
The organization of structure and function cardiac chambers in vertebrates is defined by chamber-specific distinct gene expression. This peculiarity uniqueness the genetic signatures demonstrates functional resolution attributed to different heart. Altered expression chamber genes can lead individual related dysfunctions disease patho-physiologies. Information on transcriptional repertoire compartments important understand spectrum specific anomalies. We have carried out a genome wide...
An increase in the expression of estrogen receptors (ER) and expanded population ER-positive cells are two common phenotypes breast cancer. Detection aberrantly expressed ERα cancer is carried out using ERα-antibodies radiolabelled ligands to make decisions about treatment targeted therapy. Capitalizing on beneficial advantages aptamer over conventional antibody or ligand, we have identified a DNA that selectively binds facilitates detection human tissue sections. The high throughput...
The human mitochondrial genome has been reported to have a very high mutation rate as compared with the nuclear genome. A large number of mutations show significant phenotypic association and are involved in broad spectrum diseases. In recent years, there remarkable progress understanding genetics. availability next-generation sequencing (NGS) technologies not only reduced cost by orders magnitude but also provided us good quality sequences coverage, thereby enabling decoding this study, we...
Adverse drug reactions to 5-Fluorouracil(5-FU) is frequent and largely attributable genetic variations in the DPYD gene, a rate limiting enzyme that clears 5-FU. The study aims at understanding pharmacogenetic landscape of variants south Asian populations.Systematic analysis population scale genome wide datasets over 3000 Asians was performed. Independent evaluation performed small cohort patients.Our revealed significant differences allelic distribution different ethnicities.This first...
Abstract Circular RNAs (circRNAs) are transcript isoforms generated by back-splicing of exons and circularisation the transcript. Recent genome-wide maps created for circular in humans other model organisms have motivated us to explore repertoire zebrafish, a popular organism. We RNA-seq data five major zebrafish tissues - Blood, Brain, Heart, Gills Muscle. The RNA sequence reads left over after reference mapping linear transcripts were used identify unique back-spliced utilizing...
The aorta is the largest blood vessel in body, and enlargement or aneurysm of can predispose to dissection, an important cause sudden death. While rare syndromes have been identified that aortic aneurysm, common genetic basis for size remains largely unknown. By leveraging a deep learning architecture was originally developed recognize natural images, we trained model evaluate dimensions ascending descending thoracic cardiac magnetic resonance imaging. After manual annotation just 116...
Rationale: Mechanical signaling through cell–matrix interactions plays a major role in progressive vascular remodeling pulmonary arterial hypertension (PAH). MMP-8 (matrix metalloproteinase-8) is an interstitial collagenase involved regulating inflammation and fibrosis of the lung systemic vasculature, but its PAH pathogenesis remains unexplored. Objectives: To evaluate as modulator pathogenic mechanical PAH. Methods: levels were measured plasma from patients with (PH) controls by ELISA....
LncRNA-based control affects cardiac pathophysiologies like myocardial infarction, coronary artery disease, hypertrophy, and myotonic muscular dystrophy. This study used a gene-break transposon (GBT) to screen zebrafish ( Danio rerio ) for insertional mutagenesis. We identified three mutants where the GBT captured gene. One of adult viable had bradycardia (heart arrhythmia) enlarged chambers or hypertrophy; we named it “bigheart.” Bigheart mutant insertion maps grin2bb N-methyl D-aspartate...
Recent advances in the field of genomics have seen successful implementation whole exome sequencing as a rapid and efficient diagnostic strategy several genodermatoses. The aim this study was to explore potential molecular studies dystrophic epidermolysis bullosa (DEB) India. Whole performed using genomic DNA from each case bullosa, followed by massively parallel sequencing. Resulting reads were mapped human reference genome hg19. Sanger subsequently confirmed potentially pathogenic...
South Asia is home to |$\sim $|20% of the world population and characterized by distinct ethnic, linguistic, cultural genetic lineages. Only limited representative samples from region have found its place in large population-scale international genome projects. The recent availability scale data multiple populations datasets Asian countries public domain motivated us integrate into a comprehensive resource. In present study, we integrated total six encompassing 1213 human exomes genomes...