A5091715610- CRISPR and Genetic Engineering
- RNA regulation and disease
- RNA modifications and cancer
- interferon and immune responses
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- Lipid metabolism and disorders
- Antimicrobial Peptides and Activities
- Pneumonia and Respiratory Infections
International Institute of Molecular and Cell Biology
2020-2022
Institute of Genomics and Integrative Biology
2021
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2020
Indian Institute of Technology Delhi
2020
Long non-coding RNAs (lncRNAs) are emerging as key regulators of endothelial cell function. Here, we investigated the role a novel vascular endothelial-associated lncRNA (VEAL2) in regulating permeability. Precise editing veal2 loci zebrafish (veal2gib005Δ8/+ ) induced cranial hemorrhage. In vitro and vivo studies revealed that competes with diacylglycerol for interaction protein kinase C beta-b (Prkcbb) regulates its activity. Using PRKCB2 bait, identified functional ortholog humans from...
RNA interference is a powerful experimental tool for knockdown, but not all organisms are amenable. Here, we provide proof of principle demonstration that type III Csm effector complex can be used programmable mRNA transcript degradation in eukaryotes. In zebrafish, Streptococcus thermophilus (StCsm) proved effective knockdown maternally expressed EGFP germ cells Tg(ddx4:ddx4-EGFP) fish. It also led to significant, albeit less drastic, fluorescence reduction at one day postfertilization...
Acute systemic Gram-negative bacterial infections are accompanied by release of lipopolysaccharide (LPS) endotoxins into the bloodstream and an innate immune host response via well-known toll like receptor 4 (TLR4) pathway. In this, LPS associates non-covalently with TLR4 to form activated heterodimer (LPS/MD2/TLR4)2 complex in vivo, assisted a coreceptor CD14. This complexation process has been illustrated ex vivo using indirect methods such as cytokine, interleukin, TNF-∝ measurements...
Abstract Adenosine deaminases (ADARs) catalyze the deamination of adenosine to inosine, also known as A-to-I editing, in RNA. Although editing occurs widely across animals and is well studied, new biological roles are still being discovered. Here, we study role early zebrafish development. We demonstrate that Adar, orthologue mammalian ADAR1, essential for establishing antero-posterior dorso-ventral axes patterning. Genome-wide discovery reveals pervasive maternal earliest zygotic...