A5033651095- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- interferon and immune responses
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- Immune Cell Function and Interaction
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Surfactants and Colloidal Systems
- Epigenetics and DNA Methylation
- Epilepsy research and treatment
- PI3K/AKT/mTOR signaling in cancer
- Liquid Crystal Research Advancements
- Ubiquitin and proteasome pathways
- Sulfur Compounds in Biology
- Mycobacterium research and diagnosis
- Lymphoma Diagnosis and Treatment
- Pharmacological Effects and Toxicity Studies
- Circular RNAs in diseases
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Amino Acid Enzymes and Metabolism
Institute of Human Genetics
2021-2024
Polish Academy of Sciences
1999-2024
International Institute of Molecular and Cell Biology
2021-2022
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2016-2020
Institute of Molecular Physics of the Polish Academy of Sciences
1999
Recently, a number of ribosome-associated non-coding RNAs (rancRNAs) have been discovered in all three domains life. In our previous studies, we described several types rancRNAs Saccharomyces cerevisiae, derived from many cellular RNAs, including mRNAs, rRNAs, tRNAs and snoRNAs. Here, present the evidence that tRNA fragments simple eukaryotic organism S. cerevisiae directly bind to ribosomes. Interestingly, rancRNA-tRFs yeast are both, 5'- 3'-part both tRFs associate with ribosomes vitro The...
Short RNA s derived from the cleavage of tRNA molecules are observed in most organisms. Their occurrence seems to be induced by stress conditions, but still little is known about their biogenesis and functions. We find that recovery fragments depends on isolation method. Using an optimized extraction protocol northern blot hybridization technique, we show ‐derived yeast widespread 12 different growth conditions. did not observe significant stress‐dependent changes amounts pool. Instead,...
The transcription factor MYC is a proto-oncogene with well-documented essential role in the pathogenesis and maintenance of several types cancer. binds to specific E-box sequences genome regulate gene expression cell-type- developmental-stage-specific manner. To date, combined analysis MYC-bound E-boxes their downstream target genes important for growth different cancer missing. In this study, we designed CRISPR/Cas9 library destroy genome-wide fashion. parallel, used Brunello knock out...
Abstract Adenosine deaminases (ADARs) catalyze the deamination of adenosine to inosine, also known as A-to-I editing, in RNA. Although editing occurs widely across animals and is well studied, new biological roles are still being discovered. Here, we study role early zebrafish development. We demonstrate that Adar, orthologue mammalian ADAR1, essential for establishing antero-posterior dorso-ventral axes patterning. Genome-wide discovery reveals pervasive maternal earliest zygotic...
Abstract Burkitt lymphoma (BL) is a highly aggressive that mainly affects children and young adults. Chemotherapy effective in BL patients but the outcome adults less satisfactory. Therefore, there need to enhance cytotoxic effect of drugs used treatment. Glutathione (GSH) an important antioxidant involved processes such as regulation oxidative stress drug detoxification. Elevated GSH levels have been observed many cancers were associated with chemoresistance. We previously identified GCLC,...
AMA Kasprzyk M, Brola W, Wendorff J. Assessment of clinical risk factors for drug-resistant epilepsy in children and teenagers. Medical Studies/Studia Medyczne. 2014;30(3):141-147. doi:10.5114/ms.2014.45418. APA Kasprzyk, M., Brola, W., & Wendorff, (2014). Medyczne, 30(3), 141-147. https://doi.org/10.5114/ms.2014.45418 Chicago Marta, Waldemar Janusz Wendorff. 2014. "Assessment teenagers". Medyczne 30 (3): Harvard pp.141-147. MLA Marta et al. teenagers." vol. 30, no. 3, 2014, pp. Vancouver
Chromosomal translocations in non-Hodgkin lymphoma (NHL) result activation of oncogenes by placing them under the regulation immunoglobulin heavy chain (IGH) super-enhancers. Aberrant expression translocated induced enhancer activity can contribute to lymphomagenesis. The role IGH enhancers normal B-cell development is well established, but knowledge regarding precise mechanisms their involvement control limited. goal this project was define critical regions regulatory elements and identify...
Eukaryotic genomes contain several types of recurrent sequence motifs, e.g. transcription factor miRNA binding sites, repetitive elements. CRISPR/Cas9 can facilitate identification and study crucial motifs. We present transCRISPR, the first online tool dedicated to search for motifs in user-provided genomic regions design optimal sgRNAs targeting them. Users obtain chosen up tens thousands target 30 genomes, either Cas9 or dCas9 system. TransCRISPR provides user-friendly tables...
B-cell non-Hodgkin lymphoma (NHL) is among the ten most common malignancies. Survival rates range from very poor to over 90% and highly depend on stage subtype. Characteristic features of NHL are recurrent translocations juxtaposing an oncogene (e.g. MYC, BCL2) enhancers in immunoglobulin heavy chain (IGH) locus. proliferation many lymphomas expression translocated oncogene. Thus, targeting IGH as anti-lymphoma treatment seems a promising strategy. Recently, small molecule -...
Abstract The paper presents some basic physical properties (static electric permittivities, refraction indices, density and viscosity) of 2-chloro-4-n’-alkylphenyl esters 4-n-alkylbicyclo[2,2,2] octane-1-carboxylic acids (n’=7, n = 5 7) which are, at room temperature, nematics with a negative dielectric an-isotropy. On the basis temperature dependence principal static permittivities ε ‖ (T) e ⊥ nematics, using Maier-Meier equations, angle between dipole moment vector long axis mesogenic...
ABSTRACT The transcription factor MYC is a proto-oncogene with well-documented essential role in the pathogenesis and maintenance of several types cancer. binds to specific E-box sequences genome regulate gene expression cell type- developmental stage-specific manner. To date, comprehensive analysis direct targets roles different cancer missing. enable identification functional binding sites corresponding target genes, we designed CRISPR/Cas9 library destroy genome-wide fashion. In parallel,...
ABSTRACT Eukaryotic genomes contain several types of recurrent DNA motifs, e.g. transcription factor miRNA binding sites, repetitive elements. CRISPR/Cas9 can facilitate identification and study crucial motifs. We present transCRISPR, the first online tool dedicated to search for sequence motifs in user-provided genomic regions design optimal sgRNAs targeting them. Users obtain chosen up tens thousands target 30 genomes, either Cas9 or dCas9 system. TransCRISPR provides user-friendly tables...
Abstract Adenosine deaminases (ADARs) catalyze the deamination of adenosine to inosine, also known as A-to-I editing, in RNA. Although editing occurs widely across animals, and is well studied, new biological roles are still being discovered. Here, we study role early zebrafish development. We demonstrate that Adar, orthologue mammalian ADAR1, essential for establishing antero-posterior dorso-ventral axes patterning. Genome-wide discovery revealed pervasive maternal earliest zygotic...