A5059448339- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Photosynthetic Processes and Mechanisms
- Protein Degradation and Inhibitors
- Cancer-related molecular mechanisms research
- PI3K/AKT/mTOR signaling in cancer
- Viral-associated cancers and disorders
- Circular RNAs in diseases
- Biotin and Related Studies
- Plant Stress Responses and Tolerance
- Lymphoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- DNA Repair Mechanisms
- interferon and immune responses
- Mycobacterium research and diagnosis
- Agriculture, Plant Science, Crop Management
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Plant Pathogens and Resistance
- Single-cell and spatial transcriptomics
Institute of Human Genetics
2021-2024
Polish Academy of Sciences
2021-2024
Adam Mickiewicz University in Poznań
2015-2022
The influence of the histone variant H2A.Z on transcription remains a long-standing conundrum. Here, by analyzing actin-related protein6 mutant, which is impaired in deposition, and profiling stress conditions, we investigated impact this gene expression Arabidopsis thaliana We demonstrate that arp6 mutant exhibits anomalies response to osmotic stress. Indeed, stress-responsive genes are overrepresented among those hyperactive arp6. In wild-type plants, these exhibit high levels body....
Abstract Nucleosomal acetyltransferase of H4 (NuA4) is an essential transcriptional coactivator in eukaryotes, but remains poorly characterized plants. Here, we describe Arabidopsis homologs the NuA4 scaffold proteins Enhancer Polycomb-Like 1 (AtEPL1) and Esa1-Associated Factor (AtEAF1). Loss AtEAF1 results inhibition growth chloroplast development. These effects are stronger Atepl1 mutant further enhanced by loss Golden2-Like (GLK) transcription factors, suggesting that activates nuclear...
Histone acetyltransferase complex NuA4 and histone variant exchanging SWR1 are two chromatin modifying complexes which act cooperatively in yeast share some intriguing structural similarities. Protein subunits of SWR1-C highly conserved across eukaryotes, but form different multiprotein arrangements. For example, the human TIP60-p400 consists homologues both subunits, combining necessary for acetylation exchange. It is currently not known what protein formed by plant subunits. We report on...
The transcription factor MYC is a proto-oncogene with well-documented essential role in the pathogenesis and maintenance of several types cancer. binds to specific E-box sequences genome regulate gene expression cell-type- developmental-stage-specific manner. To date, combined analysis MYC-bound E-boxes their downstream target genes important for growth different cancer missing. In this study, we designed CRISPR/Cas9 library destroy genome-wide fashion. parallel, used Brunello knock out...
Eukaryotic genomes contain several types of recurrent sequence motifs, e.g. transcription factor miRNA binding sites, repetitive elements. CRISPR/Cas9 can facilitate identification and study crucial motifs. We present transCRISPR, the first online tool dedicated to search for motifs in user-provided genomic regions design optimal sgRNAs targeting them. Users obtain chosen up tens thousands target 30 genomes, either Cas9 or dCas9 system. TransCRISPR provides user-friendly tables...
B-cell non-Hodgkin lymphoma (NHL) is among the ten most common malignancies. Survival rates range from very poor to over 90% and highly depend on stage subtype. Characteristic features of NHL are recurrent translocations juxtaposing an oncogene (e.g. MYC, BCL2) enhancers in immunoglobulin heavy chain (IGH) locus. proliferation many lymphomas expression translocated oncogene. Thus, targeting IGH as anti-lymphoma treatment seems a promising strategy. Recently, small molecule -...
ABSTRACT The transcription factor MYC is a proto-oncogene with well-documented essential role in the pathogenesis and maintenance of several types cancer. binds to specific E-box sequences genome regulate gene expression cell type- developmental stage-specific manner. To date, comprehensive analysis direct targets roles different cancer missing. enable identification functional binding sites corresponding target genes, we designed CRISPR/Cas9 library destroy genome-wide fashion. In parallel,...
Chromatin Affinity Purification (ChAP) is widely used to study chromatin architecture and protein complexes interacting with DNA. Here we present an efficient method for ChAP from Arabidopsis thaliana rosette leaves, in which vivo biotinylation system used. The digested by Micrococcal Nuclease (MNase), hence the distribution of nucleosomes also achieved. was initially developed Drosophila melanogaster ( Mito et al., 2005 ), but presented protocol has been specifically Sura 2017 ).
Abstract NuA4, an essential histone acetyltransferase complex, is required for efficient transcription in eukaryotes. Using genome editing, genomic approaches and biochemical assays, we characterized plant homologues of two key components this EPL1 EAF1 Arabidopsis thaliana . Surprisingly, found that loss AtEPL1, which necessary enzymatic activity not lethal. Contrary to yeast, mutants lacking AtEAF1, responsible complex targeting, display severe pleiotropic phenotype copies Atepl1 Ateaf1...
ABSTRACT Eukaryotic genomes contain several types of recurrent DNA motifs, e.g. transcription factor miRNA binding sites, repetitive elements. CRISPR/Cas9 can facilitate identification and study crucial motifs. We present transCRISPR, the first online tool dedicated to search for sequence motifs in user-provided genomic regions design optimal sgRNAs targeting them. Users obtain chosen up tens thousands target 30 genomes, either Cas9 or dCas9 system. TransCRISPR provides user-friendly tables...