A5052003320- Cancer-related molecular mechanisms research
- Parasites and Host Interactions
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Congenital heart defects research
- RNA Research and Splicing
- Helminth infection and control
- Coccidia and coccidiosis research
- Physiological and biochemical adaptations
- Parasite Biology and Host Interactions
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Parasitic infections in humans and animals
- Developmental Biology and Gene Regulation
- Genomics and Phylogenetic Studies
International Institute of Molecular and Cell Biology
2017-2022
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2020
Warsaw University of Life Sciences
2014-2016
Zebrafish, a popular organism for studying embryonic development and modeling human diseases, has so far lacked systematic functional annotation program akin to those in other animal models. To address this, we formed the international DANIO-CODE consortium created central repository store process zebrafish developmental genomic data. Our data coordination center ( https://danio-code.zfin.org ) combines total of 1,802 sets unpublished re-analyzed published data, which used improve existing...
ABSTRACT In the earliest stages of animal development following fertilization, maternally deposited mRNAs direct biological processes to point zygotic genome activation (ZGA). These maternal undergo cytoplasmic polyadenylation (CPA), suggesting translational control their activation. To elucidate role CPA during embryogenesis, we performed genome-wide polysome profiling at several zebrafish development. Our analysis revealed a correlation between and polysome-association dynamics,...
RNA interference is a powerful experimental tool for knockdown, but not all organisms are amenable. Here, we provide proof of principle demonstration that type III Csm effector complex can be used programmable mRNA transcript degradation in eukaryotes. In zebrafish, Streptococcus thermophilus (StCsm) proved effective knockdown maternally expressed EGFP germ cells Tg(ddx4:ddx4-EGFP) fish. It also led to significant, albeit less drastic, fluorescence reduction at one day postfertilization...
The atrioventricular canal (AVC) is the site where key structures responsible for functional division between heart regions are established, most importantly, (AV) conduction system and cardiac valves. To elucidate mechanism underlying AVC development function, we utilized transgenic zebrafish line sqet31Et expressing EGFP in to isolate this cell population profile its transcriptome at 48 72 hpf. exhibits hallmarks of mammalian AV node, including expression genes implicated those encoding...
Background The parasitic trematode Fasciola hepatica evades host immune defenses through secretion of various immunomodulatory molecules. Fatty Acid Binding Proteins ( fh FABPs) are among the main excreted/secreted proteins and have been shown to display anti-inflammatory properties. However, little is currently known regarding their impact on dendritic cells (DCs) subsequent capacity prime specific CD4 + T cell subsets. Methodology/Principal Findings effects both native F. extracts...
Abstract Zebrafish, a popular model for embryonic development and modelling human diseases, has so far lacked systematic functional annotation programme akin to those in other animal models. To address this, we formed the international DANIO-CODE consortium created first central repository store process zebrafish developmental genomic data. Our Data Coordination Center ( https://danio-code.zfin.org ) combines total of 1,802 sets unpublished reanalysed published genomics data, which used...
Abstract Background Identifying enhancers and deciphering their putative roles represent a major step to better understand the mechanism of metazoan gene regulation, development, role regulatory elements in disease. Comparative genomics transgenic assays have been used with some success identify critical regions that are involved regulating spatiotemporal expression genes during embryogenesis. Results We identified two novel tetrapod‐teleost conserved noncoding within vicinity zic3 zic6 loci...
Abstract Background The parasitic trematode Fasciola hepatica evades host immune defenses through secretion of various immunomodulatory molecules. Fatty Acid Binding Proteins ( fh FABPs) are among the main excreted/secreted proteins and have been shown to display anti-inflammatory properties. However, little is currently known regarding their impact on dendritic cells (DCs) subsequent capacity prime specific CD4 + T cell subsets. Methodology/Principal Findings effects both native F. extracts...
Abstract The atrioventricular canal (AVC) is an essential feature of the heart, which separates atrium from ventricle. During heart morphogenesis, it a hub molecular processes necessary for distinguishing regions; most importantly, formation AV conduction system and cardiac valves. To better understand underlying AVC development function, we utilized transgenic zebrafish line sqet31Et with EGFP expression in region to isolate this cell population by FACS profiled its transcriptome RNA-seq at...