A5055331285- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Single-cell and spatial transcriptomics
- Physiological and biochemical adaptations
- RNA modifications and cancer
- RNA regulation and disease
- RNA Interference and Gene Delivery
- interferon and immune responses
- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
International Institute of Molecular and Cell Biology
2012-2024
We utilized scRNA-seq to delineate the diversity of cell types in zebrafish heart. Transcriptome profiling over 50,000 cells at 48 and 72 hpf defined least 18 discrete lineages developing Utilizing well-established gene signatures, we identified a population likely be primary pacemaker characterized transcriptome profile defining this critical type. Two previously uncharacterized genes,
Abstract Ribonucleases (RNases) play a critical role in RNA processing and degradation by hydrolyzing phosphodiester bonds (exo- or endonucleolytically). Many RNases that cut internally exhibit substrate specificity, but their target sites are usually limited to one few specific nucleotides single-stranded often context of particular three-dimensional structure the substrate. Thus far, no RNase counterparts restriction enzymes have been identified which could cleave double-stranded (dsRNA)...
Ribonucleases (RNases) are valuable tools applied in the analysis of RNA sequence, structure and function. Their substrate specificity is limited to recognition single bases or distinct secondary structures substrate. Currently, there no RNases available for purely sequence-dependent fragmentation RNA. Here, we report development a new enzyme that cleaves strand DNA–RNA hybrids 5 nt from nonanucleotide sequence. The was constructed by fusing two functionally independent domains, RNase HI,...
Abstract Adenosine deaminases (ADARs) catalyze the deamination of adenosine to inosine, also known as A-to-I editing, in RNA. Although editing occurs widely across animals and is well studied, new biological roles are still being discovered. Here, we study role early zebrafish development. We demonstrate that Adar, orthologue mammalian ADAR1, essential for establishing antero-posterior dorso-ventral axes patterning. Genome-wide discovery reveals pervasive maternal earliest zygotic...
The atrioventricular canal (AVC) is the site where key structures responsible for functional division between heart regions are established, most importantly, (AV) conduction system and cardiac valves. To elucidate mechanism underlying AVC development function, we utilized transgenic zebrafish line sqet31Et expressing EGFP in to isolate this cell population profile its transcriptome at 48 72 hpf. exhibits hallmarks of mammalian AV node, including expression genes implicated those encoding...
Type C2H2 zinc fingers bind a variety of substrates, specific sequences in the double-stranded DNA counting among them. Engineering efforts led to discovery set general rules that enable obtaining modules almost any given sequence. The objective this work was determine an analogical for binding DNA-RNA hybrids using directed evolution ZfQQR finger. target regions included amino acid residues directly interact with substrate and linkers between finger modules.The performed selection based on...
Abstract The heart is comprised of a variety specialized cell types that work in unison to maintain blood flow. Here we utilized scRNA-seq analysis delineate the diversity cardiac zebrafish. With growing use zebrafish model human biology, deeper insight into its complex cellular composition critical for better understanding function, development, and associated malformations. We present high resolution atlas single cells transcriptomics, consisting over 50 000 representing building blocks at...
Abstract Objective Type C2H2 zinc fingers bind a variety of substrates among which, are specific sequences in the double-stranded DNA. Engineering efforts led to discovery set general rules that enable obtaining modules almost any given sequence. The objective this work was determine an analogical for binding DNA-RNA hybrid using directed evolution ZfQQR finger. target regions included amino acid residues directly interact with substrate and linkers between Results performed selection based...
Abstract Objective Type C2H2 zinc fingers bind a variety of substrates, specific sequences in the double-stranded DNA counting among them. Engineering efforts led to discovery set general rules that enable obtaining modules almost any given sequence. The objective this work was determine an analogical for binding DNA-RNA hybrids using directed evolution ZfQQR finger. target regions included amino acid residues directly interact with substrate and linkers between finger modules. Results...
Abstract Objective Type C2H2 zinc fingers bind a variety of substrates, specific sequences in the double-stranded DNA counting among them. Engineering efforts led to discovery set general rules that enable obtaining modules almost any given sequence. The objective this work was determine an analogical for binding DNA-RNA hybrids using directed evolution ZfQQR finger. target regions included amino acid residues directly interact with substrate and linkers between finger modules. Results...
Abstract The atrioventricular canal (AVC) is an essential feature of the heart, which separates atrium from ventricle. During heart morphogenesis, it a hub molecular processes necessary for distinguishing regions; most importantly, formation AV conduction system and cardiac valves. To better understand underlying AVC development function, we utilized transgenic zebrafish line sqet31Et with EGFP expression in region to isolate this cell population by FACS profiled its transcriptome RNA-seq at...