A5054624680- Neuroinflammation and Neurodegeneration Mechanisms
- Amino Acid Enzymes and Metabolism
- Cytokine Signaling Pathways and Interactions
- Chronic Myeloid Leukemia Treatments
- Prion Diseases and Protein Misfolding
- Cancer-related molecular mechanisms research
- Alzheimer's disease research and treatments
- Chemokine receptors and signaling
- Neurobiology and Insect Physiology Research
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- Medical Imaging Techniques and Applications
- Epigenetics and DNA Methylation
- Immune cells in cancer
- PI3K/AKT/mTOR signaling in cancer
- RNA modifications and cancer
- Ion Channels and Receptors
- Genomics and Chromatin Dynamics
- Retinal Development and Disorders
- Congenital heart defects research
- Cancer-related Molecular Pathways
- Glioma Diagnosis and Treatment
- Physiological and biochemical adaptations
International Institute of Molecular and Cell Biology
2021-2024
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2015-2022
Polish Academy of Sciences
2019-2022
Abstract Prions, proteins that can convert between structurally and functionally distinct states serve as non-Mendelian mechanisms of inheritance, were initially discovered only known in eukaryotes, consequently considered to likely be a relatively late evolutionary acquisition. However, the recent discovery prions bacteria viruses has intimated potentially more ancient origin. Here, we provide evidence prion-forming domains exist domain archaea, last life left unexplored with regard prions....
The integrated stress response (ISR) facilitates cellular adaptation to unfavorable conditions by reprogramming the response. ISR activation was reported in neurological disorders and solid tumors; however, function of its role as a possible therapeutic target hematological malignancies still remain largely unexplored. Previously, we showed that is activated chronic myeloid leukemia (CML) cells correlates with blastic transformation tyrosine kinase inhibitor (TKI) resistance. Moreover,...
Gliosarcoma is a very rare brain tumor reported to be variant of glioblastoma (GBM), IDH-wildtype. While differences in molecular and histological features between gliosarcoma GBM were reported, detailed information on the genetic background this lacking. We intend fill knowledge gap by complex analysis somatic mutations, indels, copy number variations, translocations gene expression patterns gliosarcomas. Using next generation sequencing, we determined variations (CNVs) 10 Six tumors have...
RBf2 is a recently evolved retinoblastoma family member in Drosophila that differs from RBf1, especially the C-terminus. To investigate whether unique features of contribute to diverse roles gene regulation, we performed chromatin immunoprecipitation sequencing for both and RBf1 embryos. A previous model RB-E2F interactions suggested binds dE2F1 or dE2F2, whereas restricted binding dE2F2; however, found targets approximately twice as many genes RBf1. Highly enriched among were ribosomal...
The atrioventricular canal (AVC) is the site where key structures responsible for functional division between heart regions are established, most importantly, (AV) conduction system and cardiac valves. To elucidate mechanism underlying AVC development function, we utilized transgenic zebrafish line sqet31Et expressing EGFP in to isolate this cell population profile its transcriptome at 48 72 hpf. exhibits hallmarks of mammalian AV node, including expression genes implicated those encoding...
Abstract Microglia are brain‐resident, myeloid cells that play important roles in health and brain pathologies. Herein, we report a comprehensive, replicated, false discovery rate‐controlled dataset of DNase‐hypersensitive (DHS) open chromatin regions for rat microglia. We compared the landscapes untreated primary microglial cultures stimulated 6 hr with either glioma‐conditioned medium (GCM) or lipopolysaccharide (LPS). Glioma‐secreted factors induce proinvasive immunosuppressive activation...
Stromal interaction molecules (STIMs) are endoplasmic reticulum-resident proteins that regulate Ca2+ homeostasis and signaling by store-operated calcium entry (SOCE). The different properties functions of STIM1 STIM2 have been described mostly based on work in vitro. knockout mice do not survive until adulthood. Therefore, we generated characterized stim2a stim2b double-knockout zebrafish. (stim2a;stim2b)−/− zebrafish did any apparent morphological phenotype. However, RNA sequencing revealed...
Abstract Prions are proteins capable of acquiring an alternate conformation that can then induce additional copies to adopt this same conformation. Although initially discovered in relation mammalian disease, subsequent studies have revealed the presence prions Bacteria and Viruses, suggesting ancient evolutionary origin. Here we explore whether exist Archaea - last domain life left unexplored with regard prions. After searching for potential prion-forming protein sequences computationally,...
Abstract Calcium is involved in vision processes the retina and implicated various pathologies, including glaucoma. Rod cells rely on store-operated calcium entry (SOCE) to safeguard against prolonged lowering of intracellular ion concentrations. Zebrafish that lacked endoplasmic reticulum Ca 2+ sensor Stim2 ( stim2 knockout [KO]) exhibited impaired lower light perception-related gene expression. We sought understand mechanisms are responsible for impairment KO zebrafish. The single-cell RNA...
Abstract Integrated Stress Response (ISR) facilitates cellular adaptation to variable environmental conditions by reprogramming response. Activation of ISR was reported in neurological disorders and solid tumours, but its function hematological malignancies remains largely unknown. Previously we showed that is activated chronic myeloid leukemia (CML) CD34+ cells, activity correlates with disease progression imatinib resistance. Here demonstrate inhibition small molecule ISRIB, not PERK...
Abstract Malignant gliomas represent over 70% of primary brain tumors and the most deadly is glioblastoma (GBM, WHO grade IV), due to frequent dysfunctions tumor suppressors or/and oncogenes. Recent whole genome studies demonstrated that besides genetic alterations, epigenetic contribute development progression. Alterations in genes encoding enzyme/protein or aberrations modification pattern have been found lower grade, yet no driver was identified GBM. We sought identify different...