A5078590931- Chronic Myeloid Leukemia Treatments
- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Acute Myeloid Leukemia Research
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Microtubule and mitosis dynamics
- Animal Genetics and Reproduction
- Nuclear Receptors and Signaling
- Chronic Lymphocytic Leukemia Research
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
- Genetics and Neurodevelopmental Disorders
- Telomeres, Telomerase, and Senescence
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Receptor Mechanisms and Signaling
- Veterinary Oncology Research
- Nuclear Structure and Function
- Tryptophan and brain disorders
- PI3K/AKT/mTOR signaling in cancer
- Wnt/β-catenin signaling in development and cancer
- Dietary Effects on Health
- Retinal Development and Disorders
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2011-2024
Polish Academy of Sciences
2016-2024
Temple University
2002-2011
Abstract Intercellular communication within the bone marrow niche significantly promotes leukemogenesis and provides protection of leukemic cells from therapy. Secreted factors, intercellular transfer mitochondria receptor–ligand interactions have been shown as mediators this protection. Here we report that tunneling nanotubes (TNTs)—long, thin membranous structures, which identified a novel mode cross-talk—are formed in presence stroma mediate cellular vesicles to cells. Importantly,...
Anticancer therapies that induce DNA damage tend to trigger senescence in cancer cells, a process known as therapy-induced (TIS). Such cells may undergo atypical divisions, thus contributing tumor re-growth. Accumulation of senescent reduces survival patients after chemotherapy. As interplays with autophagy, dynamic recycling process, we sought study whether inhibition autophagy interferes divisions TIS cells. We exposed human colon HCT116 repeated cycles chemotherapeutic agent - doxorubicin...
The integrated stress response (ISR) facilitates cellular adaptation to unfavorable conditions by reprogramming the response. ISR activation was reported in neurological disorders and solid tumors; however, function of its role as a possible therapeutic target hematological malignancies still remain largely unexplored. Previously, we showed that is activated chronic myeloid leukemia (CML) cells correlates with blastic transformation tyrosine kinase inhibitor (TKI) resistance. Moreover,...
Abstract Severe psychological trauma triggers genetic, biochemical and morphological changes in amygdala neurons, which underpin the development of stress-induced behavioural abnormalities, such as high levels anxiety. miRNAs are small, non-coding RNA fragments that orchestrate complex neuronal responses by simultaneous transcriptional/translational repression multiple target genes. Here we show miR-483-5p male mice counterbalances structural, functional consequences stress to promote a...
Induction of mitotic catastrophe through the disruption microtubules is an established target in cancer therapy. However, molecular mechanisms determining and following apoptotic or non-apoptotic cell death remain poorly understood. Moreover, many existing drugs targeting tubulin, such as vincristine, have reduced efficacy, resulting from poor solubility physiological conditions. Here, we introduce a novel small molecule 2-aminoimidazoline derivative—OAT-449, synthetic water-soluble tubulin...
Chronic myeloid leukemia (CML) cells circulate between blood and bone marrow niche, representing different microenvironments. We studied the role of two RNA-binding proteins, T-cell-restricted intracellular antigen (TIAR), fragile X mental retardation protein (FMRP) in regulation translation CML residing settings mimicking peripheral microenvironment (PBM) (BMM). The outcomes showed how conditions shaped process through TIAR FMRP activity, considering its relevance therapy resistance....
Feline injection site sarcomas (FISS) are malignant skin tumors with high recurrence rates despite the primary treatment of radical surgical resections. Adjunctive radiotherapy or chemotherapy doxorubicin is mostly ineffective. Cellular and molecular causes multidrug resistance, specific physio-chemical properties solid impairing drug transport, tumor microenvironment have been indicated for causing standard failure. Gold nanoparticles promising imaging tools, nanotherapeutics, delivery...
Abstract Programmed cell death involving gene regulation and de novo protein synthesis is a major component of both normal development number disease conditions. Hence, knowledge its mechanisms, especially transcription factors, that regulate expression the genes involved in neurodegenerative disorders great importance. cAMP‐responsive element–binding (CREB) has repeatedly been implicated neuronal survival. In present study we showed inducible cAMP early repressor (ICER), an endogenous CREB...
CREB activation and CREB-dependent signaling pathways are crucial for neuronal survival. The term ICER (inducible cAMP early repressor) refers to four protein isoforms that all endogenous, inducible antagonists of CREB. Jaworski others (2003) have previously shown one those isoforms, IIg, is highly expressed in apoptotic neurons vitro its overexpression evokes death. In this study we investigated the role cortical culture, comparing their expression level serum-deprived/MK-801-treated...
Cell senescence - an irreversible proliferation arrest is one of the possible cellular responses to stress. There a vast variety stimuli, extrinsic and intrinsic, known induce senescence, several molecular pathways involved in process; yet much still remains be explained. Senescent cells can communicate with neighboring through secreted factors such as cytokines chemokines. Several years ago it was shown that also more direct manner by exchange proteins via bridges (CBs). Recent studies show...
Local and regulated expression of exogenous genes in the central nervous system is one major challenges modern neuroscience. We have approached this issue by applying inducible tetracycline to regulate EGFP reporter gene double transgenic rats. obtained a strong induction only male testes, which correlated with high level rtTA organ. To overcome problem lack protein rat brain, we delivered Tet activator lentiviral vectors into dentate gyrus hippocampus As result, after systemic application...
Abstract Hormesis is defined as dose response phenomenon characterized by low-dose stimulation and high-dose inhibition (Calabrese & Mattson, 2017). To date, low doses of several stressors (intermittent fasting, caloric restriction or selected phytochemicals) have been shown to exert beneficial effects on health (Martin et al., 2006). In the present study, we aimed determine hormetic factors in a series diets used mice. We found that animals fed high-sugar diet (HSD) high-fat (HFD)...
<title>Abstract</title> Chitinase-3-like protein 1 (CHI3L1) is a secreted, non-enzymatic glycoprotein that interacts with cell-surface and extracellular-matrix proteins, proteoglycans, polysaccharides. Many studies reported the overexpression of CHI3L1 in various cancers, but its exact role tumorigenesis/cancer progression remains elusive. We performed comprehensive analysis <italic>CHI3L1</italic> expression public repositories including single-cell RNAseq datasets to determine cellular...
Transgenic animal models are fundamentally important for modern biomedical research. The incorporation of foreign genes into early mouse or rat embryos is an invaluable tool gene function analysis in living organisms. standard transgenesis method based on microinjecting DNA fragments a pronucleus fertilized oocyte. This technique widely used mice but remains relatively inefficient and technically demanding other species. transgene can also be introduced one-cell-stage via lentiviral...
Abstract Integrated Stress Response (ISR) facilitates cellular adaptation to variable environmental conditions by reprogramming response. Activation of ISR was reported in neurological disorders and solid tumours, but its function hematological malignancies remains largely unknown. Previously we showed that is activated chronic myeloid leukemia (CML) CD34+ cells, activity correlates with disease progression imatinib resistance. Here demonstrate inhibition small molecule ISRIB, not PERK...
Transgenic animal models are fundamentally important for modern biomedical research. The incorporation of foreign genes into early mouse or rat embryos is an invaluable tool gene function analysis in living organisms. standard transgenesis method based on microinjecting DNA fragments a pronucleus fertilized oocyte. This technique widely used mice but remains relatively inefficient and technically demanding other species. transgene can also be introduced one-cell-stage via lentiviral...