A5010184457- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Regulation of Appetite and Obesity
- Circular RNAs in diseases
- Health and Lifestyle Studies
- Adipose Tissue and Metabolism
- Forensic Anthropology and Bioarchaeology Studies
- Neurogenesis and neuroplasticity mechanisms
- Bone and Dental Protein Studies
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2020-2022
Polish Academy of Sciences
2020-2022
Obesity is a growing medical and social problem worldwide. The control of energy homeostasis in the brain achieved by various regions including arcuate hypothalamic nucleus (ARH). latter comprises number neuronal populations first order metabolic neurons, appetite-stimulating agouti-related peptide (AgRP) neurons appetite-suppressing proopiomelanocortin (POMC) neurons. Using an vivo reductionist approach POMCCre-dependent CRISPR-Cas9, we demonstrate that miR-15a-5p protects from obesity....
Transgenic animal models are fundamentally important for modern biomedical research. The incorporation of foreign genes into early mouse or rat embryos is an invaluable tool gene function analysis in living organisms. standard transgenesis method based on microinjecting DNA fragments a pronucleus fertilized oocyte. This technique widely used mice but remains relatively inefficient and technically demanding other species. transgene can also be introduced one-cell-stage via lentiviral...
Transgenic animal models are fundamentally important for modern biomedical research. The incorporation of foreign genes into early mouse or rat embryos is an invaluable tool gene function analysis in living organisms. standard transgenesis method based on microinjecting DNA fragments a pronucleus fertilized oocyte. This technique widely used mice but remains relatively inefficient and technically demanding other species. transgene can also be introduced one-cell-stage via lentiviral...