A5082011854- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- DNA Repair Mechanisms
- Memory and Neural Mechanisms
- DNA and Nucleic Acid Chemistry
- Image Processing Techniques and Applications
- Image and Signal Denoising Methods
- Metal complexes synthesis and properties
- Marine Sponges and Natural Products
- Receptor Mechanisms and Signaling
- X-ray Diffraction in Crystallography
- Pancreatic function and diabetes
- Crystallization and Solubility Studies
- Advanced biosensing and bioanalysis techniques
- Advanced Data Compression Techniques
- Cardiac electrophysiology and arrhythmias
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Wastewater Treatment and Nitrogen Removal
- Genomics and Chromatin Dynamics
- Supramolecular Chemistry and Complexes
- Lipid metabolism and biosynthesis
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular transport and secretion
Virginia Commonwealth University
2019-2025
Polish Academy of Sciences
2015-2024
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2014-2024
Children's Hospital of Richmond at VCU
2019-2024
Indiana University School of Medicine
2020
Purdue University West Lafayette
2005-2018
University of Warsaw
2018
Royal College of Surgeons in Ireland
2010-2017
University of Silesia in Katowice
2002-2016
Jagiellonian University
1998-2013
Abstract Background Bioreduction of water‐soluble tetrazolium salts (e.g., MTS, XTT, and MTT) to their respective formazans is generally regarded as an indicator cell “redox activity.” The reaction attributed mainly mitochondrial enzymes electron carriers. However, MTT reduction may also be catalyzed by a number other nonmitochondrial enzymes. goal this work was establish the sites in intact HepG2 human hepatoma cells culture. Methods In order subcellular localization MTT, we imaged...
Abstract Structural plasticity of dendritic spines is thought to underlie memory formation. Size a spine considered proportional the size its postsynaptic density (PSD), number glutamate receptors and synaptic strength. However, whether this correlation true for all volumes, remains stable during plasticity, largely unknown. In study, we take advantage 3D electron microscopy reconstruct cores PSDs from stratum radiatum area CA1 organotypic hippocampal slices. We observe that approximately...
Summary Exposure to light can destroy the ability of a molecule fluoresce. Such photobleaching limits use fluorescence and confocal microscopy in biological studies. Loss decreases signal‐to‐noise ratio so image resolution; it also prevents acquisition meaningful data late during repeated scanning (e.g. when collecting three‐dimensional images). The aim this work was investigate role oxygen fluorophores bound DNA fixed cells, explore whether anoxia could minimize such bleaching. Anoxia...
Colorectal cancer (CRC) is the second leading cause of death among patients in Northern countries. CRC can reappear a long time after treatment. Recent clinical studies demonstrated that, response to chemotherapy, cells may undergo stress-induced premature senescence (SIPS), which typically results growth arrest. Nonetheless, these senescent were reported divide an atypical manner and thus contribute re-growth. Therefore, we examined if SIPS escape follow treatment with chemotherapeutics...
Autophagy is indispensable for the proper architecture and flawless functioning of pancreatic β-cells. A growing body evidence indicates reciprocal communication between autophagic pathways, apoptosis, intracellular lipids. The way in which elevated levels free saturated or unsaturated FAs contribute to progressive β-cell failure remains incompletely understood. Stearoyl-CoA desaturase (SCD)1, a key regulatory enzyme biosynthesis MUFAs, was shown play an important role regulation function....
Anticancer therapies that induce DNA damage tend to trigger senescence in cancer cells, a process known as therapy-induced (TIS). Such cells may undergo atypical divisions, thus contributing tumor re-growth. Accumulation of senescent reduces survival patients after chemotherapy. As interplays with autophagy, dynamic recycling process, we sought study whether inhibition autophagy interferes divisions TIS cells. We exposed human colon HCT116 repeated cycles chemotherapeutic agent - doxorubicin...
// Paulina Rybak 1 , Agnieszka Hoang Lukasz Bujnowicz 2 Tytus Bernas 3 Krzysztof Berniak Mirosław Zarębski Zbigniew Darzynkiewicz 4 Jerzy Dobrucki Department of Cell Biophysics, Faculty Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland Molecular Laboratory for Imaging Tissue Structure Function, Nencki Institute Experimental Biology PAS, Warsaw, Brander Cancer Research Pathology, New York Medical College, Valhalla, York, USA Correspondence to: Dobrucki,...
Dysregulated metabolism of bioactive sphingolipids, including ceramides and sphingosine-1-phosphate, has been implicated in cardiovascular disease, although the specific species, disease contexts, cellular roles are not completely understood. Sphingolipids produced by serine palmitoyltransferase enzyme, canonically composed 2 subunits, SPTLC1 (serine long chain base subunit 1) SPTLC2 2). Noncanonical sphingolipids a more recently described subunit, SPTLC3 3).
Abstract Structure of bacterial biofilms may be investigated using several variants scanning electron microscopy (SEM). We apply lyophilization to prepare nitrifying biofilm for conventional SEM imaging in high‐vacuum mode (CSEM). therefore replace standard fixation glutaraldehyde cross‐linking, ethanol dehydration, and critical‐point drying (CPD) with less‐invasive low‐temperature by sublimation vacuum. compare this approach with: (1) preparation fixation, CPD before CSEM, (2) cryo‐sputter...
Chromatin is the substrate for many processes in cell nucleus, including transcription, replication, and various DNA repair systems, all of which require formation multiprotein machineries on chromatin fiber. We have analyzed kinetics vivo assembly protein complex that responsible nucleotide excision (NER) mammalian cells. Assembly initiated by UV irradiation a small area after accumulation GFP-tagged NER proteins DNA-damaged measured, reflecting establishment dual-incision complex. The...
A method of quantitative analysis spatial (3D) relationship between discrete nuclear events detected by confocal microscopy is described and applied in a dependence sites DNA damage signaling (γH2AX foci) replication (EdU incorporation) cells subjected to treatments with camptothecin (Cpt) or hydrogen peroxide (H 2 O ). Cpt induces γH2AX foci, likely reporting formation double‐strand breaks (DSBs), almost exclusively at replication. This finding consistent the known mechanism induction DSBs...
We examined six types of cells that form the ovary earthworm Dendrobena veneta ogonia, prooocytes, vitellogenic oocytes, trophocytes, fully grown postvitellogenic oocytes and somatic gonad. The quantitative stereological method revealed a much higher "volume density" mitochondria in all germ-line except for cells. Fluorescent vital stain JC-1, however, showed oxidative activity than distribution active inactive within studied was assessed using computer program ImageJ. analysis luminosity...
Luminescent <italic>fac</italic>-[Re(CO)<sub>3</sub>(phen)(aspirin)]: insights into <italic>in vitro</italic> anticancer activity and confocal microscopy imaging in HeLa cells.
The updating of contextual memories is essential for survival in a changing environment. Accumulating data indicate that the dorsal CA1 area (dCA1) contributes to this process. However, cellular and molecular mechanisms fear memory remain poorly understood. Postsynaptic density protein 95 (PSD-95) regulates structure function glutamatergic synapses. Here, using dCA1-targeted genetic manipulations vivo, combined with ex vivo 3D electron microscopy electrophysiology, we identify novel,...
During chronic stress, persistent activation of cAMP-dependent protein kinase (PKA) occurs, which can contribute to protective or maladaptive changes in the heart. We sought understand effect PKA on NaV1.5 channel distribution and function cardiomyocytes using adult rat ventricular myocytes as main model. with 8CPT-cAMP okadaic acid (phosphatase inhibitor) caused an increase Na+ current amplitude without altering total level, suggesting a redistribution myocytes' surface. Biotinylation...
The air-liquid interface (ALI) culture is an important tool in pulmonary research as it models the physiological lung where epithelium apically exposed to air and basally endothelium interstitium. While there abundance of that employs primary human bronchial epithelial cells (HBECs) study larger airways, small airway (SAECs) are untapped resource comparison. Primary SAECs a valuable cell population they enable pathologies bronchioles also favorable surrogate for alveolar which invasive...
Prolonged excitation of fluorescent probes leads eventually to loss their capacity emit light. A decrease in the number detected photons reduces subsequently resolving power a fluorescence microscope. Adverse effects intensity on quality microscopic images biological specimens have been recognized, but not determined quantitatively. We propose three human-independent methods determination. These techniques require no reference and are based calculation actual resolution distance, information...
Abstract Background The actin cytoskeleton is involved in the responses of plants to environmental signals. Actin bundles play role tracks chloroplast movements activated by light. Chloroplasts redistribute response blue light mesophyll cells Nicotiana tabacum . aim this work was study relationship between and organization living tobacco cells. Chloroplast were measured photometrically as changes transmission through leaves. cytoskeleton, labeled with plastin-GFP, visualised confocal...
Epigenetic modifications such as histone and DNA methylation are highly conserved among eukaryotes, although the nuclear patterns of these vary between different species. Brassica species represent a very attractive model for analysis epigenetic changes because their differences in genome size, ploidy level, organization heterochromatin blocks. rapa B. oleracea diploid species, napus is an allotetraploid that arose from hybridization two diploids. We found H3 differ The most prominent...
Summary Neuronal survival and plasticity critically depend on constitutive activity of the transcription factor nuclear factor-κB (NF-κB). We here describe a role for small intracellular fibroblast growth homologue, homologous 1 (FHF1/FGF12), in regulation NF-κB mature neurons. FHFs have previously been described to control neuronal excitability, mutations FHF isoforms give rise form progressive spinocerebellar ataxia. Using protein-array approach, we identified FHF1b as novel interactor...
Two new neutral fac-[Re(CO)₃(phen)L] compounds (1,2), with phen = 1,10-phenanthroline and L O₂C(CH₂)₅CH₃ or O₂C(CH₂)₄C≡CH, were synthetized in one-pot procedures from fac-[Re(CO)₃(phen)Cl] the corresponding carboxylic acids, fully characterized by IR UV-Vis absorption spectroscopy, ¹H- 13C-NMR, mass spectrometry X-ray crystallography. The compounds, which display orange luminescence, used as probes for living cancer HeLa cell staining. Confocal microscopy revealed accumulation of both dyes...