A5049411755- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Cancer-related Molecular Pathways
- Ion channel regulation and function
- DNA and Nucleic Acid Chemistry
- Cancer therapeutics and mechanisms
- Plant Pathogens and Fungal Diseases
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Plant tissue culture and regeneration
- Ion Transport and Channel Regulation
- Gut microbiota and health
- Seed Germination and Physiology
- Genomics and Chromatin Dynamics
- Plant Genetic and Mutation Studies
- Protein purification and stability
- Pancreatic function and diabetes
- Molecular Biology Techniques and Applications
- Cytokine Signaling Pathways and Interactions
- Microtubule and mitosis dynamics
- Protein Interaction Studies and Fluorescence Analysis
- Bone Metabolism and Diseases
- PARP inhibition in cancer therapy
- Genomics, phytochemicals, and oxidative stress
- Metabolism, Diabetes, and Cancer
Jiamusi University
2023-2025
Guizhou Institute of Plant Protection
2025
The First Affiliated Hospital, Sun Yat-sen University
2024
Zhongshan Hospital
2024
Fudan University
2024
Sun Yat-sen University
2024
Guizhou University
2022-2024
New York Medical College
2010-2022
Nanyang Medical College
2022
East China University of Science and Technology
2011-2022
Background— Abnormally elevated blood pressure is the most prevalent risk factor for cardiovascular disease. The large-conductance, voltage- and Ca 2+ -dependent K + (BK) channel has been proposed as an important effector in control of vascular tone by linking membrane depolarization local increases cytosolic to hyperpolarizing outward currents. However, BK may also affect regulating salt fluid homeostasis, particularly adjusting renin-angiotensin-aldosterone system. Methods Results— Here we...
ABSTRACT The Wnt/β-catenin signaling pathway is essential for bone cell viability and function skeletal integrity. To determine if β-catenin in osteocytes plays a role the anabolic response to mechanical loading, 18- 24-week-old osteocyte haploinsufficient mice (Dmp1-Cre × fl/ + ; HET cKO) were compared with their fl/fl (control) littermates. Trabecular volume (BV/TV) was significantly less (58.3%) cKO females versus controls, whereas male control not different. number controls both genders,...
Osteocytes appear to mobilize calcium within minutes in response PTH injections; we have previously shown that osteocytes remove their perilacunar matrix during lactation through activation of the type 1 receptor. Mechanisms utilized by are unknown but hypothesized molecular components may be similar those used osteoclasts. Here show, using IDG-SW3 cells ATP6V0D2, an essential component vacuolar ATPase osteoclasts, and other genes associated with osteoclastic bone resorption, increase...
ABSTRACT Connexin (Cx) 43 serves important roles in bone function and development. Targeted deletion of Cx43 osteoblasts or osteocytes leads to increased osteocyte apoptosis, osteoclast recruitment, reduced biomechanical properties. forms both gap junction channels hemichannels, which mediate the communication between adjacent cells cell extracellular environments, respectively. Two transgenic mouse models driven by a DMP1 promoter with overexpression dominant negative mutants were generated...
Polysaccharides derived from Atractylodes chinensis (DC.) Koidz. (ACP), a traditional Chinese medicine, were extracted and analyzed for their structural characteristics anti-gouty nephropathy (GN) activity. Sprague-Dawley (SD) rats divided into six groups: control, model, positive three treatment groups (ACP-60-L, ACP-60-M, ACP-60-H). Treatment significantly reduced inflammatory responses renal damage, as evidenced by decreased levels of uric acid (UA), creatinine (Cr), blood urea nitrogen...
Two different mechanisms are considered to be the primary cause of aging. Cumulative DNA damage caused by reactive oxygen species (ROS), by-products oxidative phosphorylation, is one these (ROS concept). Constitutive stimulation mitogen- and nutrient-sensing mTOR/S6 signaling second mechanism (TOR The flow- laser scanning- cytometric methods were developed measure level constitutive damage/ROS- as well mTOR/S6- in individual cells. Specifically, persistent activation ATM expression γH2AX...
Abstract Cellular DNA content can be measured by flow cytometry with the aim of : (1) revealing cell distribution within major phases cycle, (2) estimating frequency apoptotic cells fractional content, and/or (3) disclosing ploidy population. In this unit, simple and universally applicable methods for staining fixed are presented, as that utilize detergents proteolytic treatment to permeabilize make accessible fluorochrome. Additionally, supravital Hoechst 33342, which is primarily used...
The pancreatic duct secretes alkaline fluid that is rich in HCO3- and poor Cl-. molecular mechanisms mediate ductal secretion are responsible for the axial gradients of Cl- along tree not well understood because H+ transport by cells have been characterized or localized. To address these questions, we microdissected intralobular, main, common segments rat duct. transporters were localized following intracellular pH while perfusing bath lumen ducts. In intralobular ducts, Na(+)-dependent...
Global (all cells in an acinus) and focal (1-2 out of 10-15 cells) stimulation pancreatic acini with bombesin or t-butyloxycarbonyl-Tyr(SO3)-Nle-Gly-Tyr-Asp-2-phenylethyl ester (CCKJ) together modulation gap junction (GJ) permeability by octanol NO2- was used to study the role GJ controlling [Ca2+]i oscillations enzyme secretion. quantitated measuring fluorescence recovery after photobleaching. Octanol at 0.5 mM markedly reduced, whereas 15 increased permeability. Focal application caused...
A permeable cell system in which Ca2+ release can be evoked by inositol 1,4,5-trisphosphate (IPa) or agonist stimulation was used to study the regulation of itself.At low concentrations, activated IP3-mediated (IMCR) with halfmaximal effect at about 15 nM.At high inhibited IMCR giving rise a biphasic [Ca"'] dependence IMCR.The activation appears mediated kinase, probably Ca"+and calmodulin-dependent protein kinase (CaMKII).Thus, required MgATP, completely blocked 0 "C, Ca"+, and CaMKII...
In Pichia pastoris, secretory proteins are folded and assembled in the endoplasmic reticulum (ER). However, upon introduction of foreign proteins, heterologous often retained cytoplasm or ER as a result suboptimal folding conditions, leading to protein aggregation. The Hsp70 Hsp40 chaperone families importantly regulate secretion proteins. it is not clear which single most important combination optimally cooperates this process. present study we evaluated role chaperones Kar2p, Sec63, YDJ1p,...
Abstract The “click chemistry” approach utilizing 5‐ethynyl‐2′‐deoxyuridine (EdU) as a DNA precursor was recently introduced to assess replication and adapted flow‐ imaging‐cytometry. In the present study, we observed that EdU, once incorporated into DNA, induces damage signaling (DDS) such phosphorylation of ATM on Ser1981, histone H2AX Ser139, p53 Ser15, Chk2 Thr68. It also perturbs progression cells through cell cycle subsequently apoptosis. These effects were in non‐small lung...
In addition to its traditional role in the regulation of calcium homeostasis and bone metabolism, vitamin D also exhibits immunomodulatory, anti-proliferative cancer preventive activities. Molecular mechanisms that confer chemo-preventive properties are poorly understood. We previously reported constitutive phosphorylation histone H2AX on Ser139 (γH2AX) activation ATM (Ser1981 phosphorylation), seen untreated normal or tumor cells predominantly S phase cell cycle, is a large extent...
ABSTRACT The dental cementum covering the tooth root is similar to bone in several respects but remains poorly understood terms of development and differentiation cementoblasts, as well potential function(s) cementocytes residing cellular cementum. It not known if cementocyte a dynamic actor metabolism, comparable osteocyte bone. Cementocytes exhibit irregular spacing lacunar shape, with fewer canalicular connections compared osteocytes. Immunohistochemistry quantitative PCR (qPCR) revealed...
The ongoing DNA damage caused by reactive oxygen species generated during oxidative metabolism is considered a key factor contributing to cell aging as well preconditioning cells neoplastic transformation. We postulated before that significant fraction of constitutive histone H2AX phosphorylation (CHP) and activation ATM (CAA) seen in untreated normal tumor occurs response such damage. In the present study, we provide further evidence support this postulate. level phosphorylation, detected...
The aim of this study was to assess the potential DNA damage response (DDR) four supravitally used biomarkers Hoechst 33342 (Ho 42), DRAQ5, DyeCycle Violet (DCV), and SYTO 17. A549 cells were exposed these at concentrations generally applied live their effect on histone H2AX (Ser 139), p53 (Ser15), ATM (Ser1981), Chk2 (Thr68) phosphorylation assessed using phospho-specific Abs. Short-term treatment with Ho 42 led modest degree activation no evidence H2AX, Chk2, or phosphorylation. However,...
We have shown before that constitutive DNA damage signaling represented by H2AX-Ser139 phosphorylation and ATM activation in untreated normal tumor cells is a reporter of the persistent replication stress induced endogenous oxidants, by-products aerobic respiration. In present study we observed exposure mitogenically stimulated lymphocytes or cell lines A549, TK6 A431 to metformin, specific activator 5'AMP-activated protein kinase (AMPK) an inhibitor mTOR signaling, resulted attenuation H2AX...
Abstract DNA topoisomerase I (Top1) and II (Top2) inhibitors are widely used to treat a variety of cancers. Their mechanism action involves stabilization otherwise transient (“cleavable”) complexes between Top1 or Top2 DNA; collisions replication forks with such stabilized lead formation double‐strand breaks (DSBs). In this study, using 5‐ethynyl‐2′deoxyuridine (EdU) as precursor, we directly assessed the relationship induction DSBs revealed γH2AX foci in A549 cells treated topotecan (Tpt)...
The role of internal stores and plasma membrane Ca2+ pumps in controlling [Ca2+]i during agonist stimulation their regulation by agonists are not well understood. We report here measurements intracellular ([Ca2+]i) extracellular ([Ca2+]o) concentrations agonist-stimulated pancreatic acini an effort to directly address these questions. Stimulation suspended Ca(2+)-free or Ca(2+)-containing medium with mobilizing resulted a typical transient increase [Ca2+]i. Thapsigargin, specific inhibitor...