A5046738079- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Facial Trauma and Fracture Management
- Bone health and treatments
- Traumatic Ocular and Foreign Body Injuries
- Genetics and Physical Performance
- Intraocular Surgery and Lenses
- Bone and Dental Protein Studies
- Cellular transport and secretion
- Wnt/β-catenin signaling in development and cancer
- Electrospun Nanofibers in Biomedical Applications
- Cardiovascular Effects of Exercise
- Respiratory Support and Mechanisms
- Exercise and Physiological Responses
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Elasticity and Material Modeling
- Quality and Safety in Healthcare
- Cellular Mechanics and Interactions
- Hedgehog Signaling Pathway Studies
- Genetic Syndromes and Imprinting
- Trauma Management and Diagnosis
- Cardiac Arrest and Resuscitation
- Genetic and Kidney Cyst Diseases
- Muscle Physiology and Disorders
Engineering Systems (United States)
2013-2020
University of Missouri–Kansas City
2011-2018
The purpose of this work was to establish a methodology enable the isolation and study osteocytes from skeletally mature young (4-month-old) old (22-month-old) mice. location deep within bone is ideal for their function as mechanosensors. However, makes observation in vivo technically difficult. Osteocytes were isolated murine long bones through process extended collagenase digestions combined with EDTA-based decalcification. A tissue homogenizer used reduce remaining fragments suspension...
ABSTRACT The Wnt/β-catenin signaling pathway is essential for bone cell viability and function skeletal integrity. To determine if β-catenin in osteocytes plays a role the anabolic response to mechanical loading, 18- 24-week-old osteocyte haploinsufficient mice (Dmp1-Cre × fl/ + ; HET cKO) were compared with their fl/fl (control) littermates. Trabecular volume (BV/TV) was significantly less (58.3%) cKO females versus controls, whereas male control not different. number controls both genders,...
Polycystin-1 (Pkd1) interacts with polycystin-2 (Pkd2) to form an interdependent signaling complex. Selective deletion of Pkd1 in the osteoblast lineage reciprocally regulates osteoblastogenesis and adipogenesis. The role Pkd2 skeletal development has not been defined. To this end, we conditionally inactivated mature osteoblasts by crossing Osteocalcin (Oc)-Cre;Pkd2+/null mice floxed (Pkd2flox/flox) mice. Oc-Cre;Pkd2flox/null (Pkd2Oc-cKO) exhibited decreased bone mineral density, trabecular...
Ballistic studies were conducted using gelatin-embedded abattoir-fresh porcine eyes suspended within clear acrylic orbits to discern the energy required produce specific ocular injuries. Paintball impact provides a robust ballistic model for isolating and quantifying role of direct blunt force in trauma.Fifty-nine orbital preparations received blows from 0.68 caliber (16-18 mm diameter/3.8 g) paintballs fired at velocities ranging 26 97 meters per second (2-13.5 J). Five additional not...
To create a computer-based numerical simulation model for comparison with empiric paintball-ocular ballistic study findings, allowing identification of the dynamic physical mechanisms (stress, strain, pressure) responsible intraocular traumatic injury accompanying blunt ocular impact. Virtual experiments models could exploit mathematical "instrumentation" to facilitate internal observation impossible alone.Models human eye structures and orbit were implemented into finite-volume Eulerian...
Though osteoporosis is a significant cause of disability worldwide, treatment with pharmacologic agents decreases risk fragility fracture. these treatments act through the bone remodeling system to improve mass, it unclear if they alter response mechanical loading at level osteocyte. This pre-clinical study determined relationship between microstructural tissue properties and osteocyte lacunar size density strain around osteocytes standard or sequential therapies. Six-month-old female...
Purpose.: Ballistic impact studies and supercomputer modeling were performed to elicit the mechanisms of optic nerve rupture that may accompany blunt ocular trauma. Methods.: Paintball responses studied with abattoir-fresh porcine eyes. Physics-based numerical code CTH was used produce robust geometric constitutive models eye orbit, providing a comparative 3-D finite volume model help determine underlying empirical ballistic observations. Results.: Among 59 specimens submitted paintball in...
When preparing tissue engineering and regenerative medicine constructs, a commonly encountered problem is the failure of seeded cells to infiltrate scaffold. In an increasing number cases, constructs are being mechanically preconditioned with expectation that preconditioning will enhance construct's maturation effectiveness by pre-exposing stimuli interest experiences in vivo. However, whether or not mechanostimulation scaffold actually results transmission remains poorly understood. The...
Accident reconstruction involving consumer products and industrial equipment often requires biomechanical and/or human factors analyses to help determine the root cause of an accident scenario. A systematic method has been established which incorporates numerous components sciences biomechanics uses scientific as framework for evaluating competing theories. Using this method, available data are gathered pertaining or incident organized in a modified Haddon matrix, with categories Man...
Cis‐Golgi protease Mbtps1, a proprotein convertase, is responsible for activating small family of transmembrane spanning b‐ZIP transcription factors. We previously demonstrated Mbtps1 required mineralization osteoblastic cultures. To understand its role in skeletal development, we conditionally deleted MBTPS1 using osteocyte restricted DMP1 Cre recombinase. Despite the absence an early bone phenotype, KO male mice abruptly begin to gain weight at maturity. At 40 weeks, knockout were 7.3g...