A5045710613- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Pancreatic function and diabetes
- Fatty Acid Research and Health
- Lipid metabolism and biosynthesis
- Metabolism, Diabetes, and Cancer
- Sphingolipid Metabolism and Signaling
- Cancer, Lipids, and Metabolism
- Diet, Metabolism, and Disease
- 3D Printing in Biomedical Research
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Cholesterol and Lipid Metabolism
- Liver Disease Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Erythrocyte Function and Pathophysiology
- Tissue Engineering and Regenerative Medicine
- Neurological diseases and metabolism
- Metabolomics and Mass Spectrometry Studies
- Diabetes Management and Research
- Pluripotent Stem Cells Research
- Lipid Membrane Structure and Behavior
- Nitric Oxide and Endothelin Effects
- Autophagy in Disease and Therapy
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2016-2025
Polish Academy of Sciences
2015-2025
BM Központi Kórház
2023
Rutgers, The State University of New Jersey
2021
Medical University of Lodz
2013
Toxicologie, Pharmacologie et Signalisation Cellulaire
2012
University of Wisconsin–Madison
2003-2009
Weatherford College
2008
Medical University of Białystok
2002-2006
San Francisco VA Medical Center
2005
Stearoyl-CoA desaturase (SCD) catalyzes the rate-limiting step in biosynthesis of monounsaturated fatty acids. Mice with a targeted disruption SCD1 isoform have reduced body adiposity, increased energy expenditure, and up-regulated expression several genes encoding enzymes acid β-oxidation liver. The mechanisms by which SCD deficiency leads to these metabolic changes are presently unknown. Here we show that phosphorylation activity AMP-activated protein kinase (AMPK), sensor regulates lipid...
Stearoyl-CoA desaturase (SCD) synthesizes oleate necessary for the biosynthesis of triglycerides and other lipids. Mice with a targeted disruption SCD1 gene are deficient in tissue have reduced expression sterol regulatory element-binding protein (SREBP) its target genes. The SREBP-1c isoform is known mediator insulin action on hepatic expression, but transcriptional effects due to glucose or fructose still unclear. We found that compared stronger inducer lipogenic causing dramatic increase...
Dietary saturated fats have often been implicated in the promotion of obesity and related disorders. It has shown recently that act through transcription factor SREBP-1c (sterol regulatory element-binding protein-1c) its requisite coactivator, peroxisome proliferator-activated receptor-gamma coactivator-1beta (PGC-1beta), to exert their pro-lipogenic effects. We show here a diet high fat stearate induces lipogenic genes wild-type mice, with induction Scd1 (stearoyl-CoA desaturase-1) gene...
We have shown previously that mice with a targeted disruption in the stearoyl-CoA desaturase 1 gene ( SCD1 -/- ) increased insulin sensitivity compared control mice. Here we show signaling muscle. The basal tyrosine phosphorylation of receptor and substrates 2 are elevated. insulin-like growth factor-1 was similar between +/+ association αp85 subunit phosphatidylinositol 3-kinase as well Akt-Ser-473 Akt-Thr-308 also elevated Interestingly, mRNA levels, protein mass, activity protein-tyrosine...
Stearoyl-CoA desaturase (SCD) has recently been shown to be a critical control point of lipid partitioning and body weight regulation. Lack SCD1 function significantly increases insulin sensitivity in skeletal muscles corrects the hypometabolic phenotype leptin-deficient ob/ ob mice, indicating direct antilipotoxic action deficiency. The mechanism underlying metabolic effects mutation is currently unknown. Here we show that deficiency reduced total ceramide content oxidative (soleus red...
There are four known stearoyl-CoA desaturase (SCD) enzyme isoforms in mouse and two humans that required for the biosynthesis of monounsaturated fatty acids, mainly oleate. SCD1 isoform plays a role regulation energy metabolism lipid synthesis, but roles other SCD have not been investigated. Here we show SCD2 is important synthesis early development survival. SCD2-deficient ( Scd2 -/- ) neonatal mice skin permeability barrier defect specific repartitioning linoleic acid from epidermal...
Stearoyl-CoA desaturase (SCD) is a microsomal enzyme involved in the biosynthesis of oleate and palmitoleate. Mice with targeted disruption SCD1 isoform (SCD1-/-) exhibit reduced adiposity increased energy expenditure. To address whether expenditure attributable to thermogenesis, we investigated effect deficiency on basal cold-induced thermogenesis. SCD1-/- mice have expression uncoupling proteins brown adipose tissue (BAT) relative controls. The beta3-adrenergic receptor (beta3-AR) was...
Stearoyl-CoA desaturase catalyzes the rate-limiting step in biosynthesis of monounsaturated fatty acids, which are required for normal rates synthesis triglycerides, cholesterol esters, and phospholipids. Mice with a targeted disruption stearoyl-CoA 1 (SCD1) isoform protected against diet leptin deficiency-induced adiposity, have increased energy expenditure, up-regulated expression hepatic genes encoding enzymes acid beta-oxidation. Because peroxisome proliferator-activated receptor-alpha...
Abstract Obesity and related metabolic pathologies represent a significant public health concern. is associated with increased oxidative stress that damages genomic mitochondrial DNA. Oxidatively-induced lesions in both DNA pools are repaired via the base-excision repair pathway, initiated by glycosylases such as 8-oxoguanine glycosylase (OGG1). Global deletion of OGG1 common polymorphisms render mice humans susceptible to disease. However, relative contribution this phenotype unknown. Here,...
The sphingomyelin-signaling pathway has been described in many tissues. Ceramide is the main second messenger this pathway. also shown to be present skeletal muscles; however, there are few data on regulation of content ceramide muscle tissue. Moreover, no particular ceramides or their composition muscles. aim study was examine and fatty acids (FA) sphingomyelin moieties activity neutral Mg 2+ -dependent sphingomyelinase different types rat at rest after exhausting exercise moderate...
Endocannabinoid signaling has been implicated in modulating insulin release from β cells of the endocrine pancreas. Cells express CB1 cannabinoid receptors (CB1Rs), and enzymatic machinery regulating anandamide 2-arachidonoylglycerol bioavailability. However, molecular cascade coupling agonist-induced receptor activation to remains unknown. By combining pharmacology genetic tools INS-1E vivo, we show that CB1R by endocannabinoids (anandamide 2-arachidonoylglycerol) or synthetic agonists...
Autophagy is indispensable for the proper architecture and flawless functioning of pancreatic β-cells. A growing body evidence indicates reciprocal communication between autophagic pathways, apoptosis, intracellular lipids. The way in which elevated levels free saturated or unsaturated FAs contribute to progressive β-cell failure remains incompletely understood. Stearoyl-CoA desaturase (SCD)1, a key regulatory enzyme biosynthesis MUFAs, was shown play an important role regulation function....
Significance Glucagon and insulin are produced in distinct cell populations within pancreatic Langerhans islets, where intercellular interactions control their production release. Modifications to the microstructure of islets implicated disease pathogenesis, but developmental rules underlying commitment segregation incompletely understood. We show that endocannabinoids (ω-6) via CB 1 cannabinoid receptors endovanilloid ligands transient receptor potential cation channel subfamily V member 1,...
Nonalcoholic fatty liver disease (NAFLD) is characterized by the development of steatosis, which can ultimately compromise function. Mitochondria are key players in obesity-induced metabolic disorders; however, distinct role hypercaloric diet constituents hepatic cellular oxidative stress and metabolism unknown. Male mice were fed either a high-fat (HF) diet, high-sucrose (HS) or combined HF plus HS (HFHS) for 16 weeks. This study shows that diets caused steatosis; HFHS induced severe...