A5066957224- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Inflammatory Bowel Disease
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Cell death mechanisms and regulation
- Receptor Mechanisms and Signaling
- Advanced Proteomics Techniques and Applications
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- Viral gastroenteritis research and epidemiology
- Bone Metabolism and Diseases
- Psoriasis: Treatment and Pathogenesis
- Cancer Research and Treatments
- interferon and immune responses
- Blood groups and transfusion
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- CAR-T cell therapy research
- Bone and Dental Protein Studies
- bioluminescence and chemiluminescence research
IMGENEX India (India)
2001-2024
Michigan State University
2024
US Biologic (United States)
2024
University of California, San Diego
2018-2020
Ecogenomics (Japan)
2018
IDEX Corporation (United States)
2000-2013
Institute of Bioinformatics
2008
Johns Hopkins University
2008
The University of Texas MD Anderson Cancer Center
1996-2004
Molecular Oncology (United States)
1996
Bax is a proapoptotic member of the Bcl-2 protein family that commits cell to undergo programmed death in response apoptotic stimuli. To gain further insights into mechanisms, we have identified novel Bax-binding protein, termed Bif-1, by using yeast two-hybrid cloning technique. Bif-1 an evolutionarily conserved cytoplasmic contains predicted Src homology 3 (SH3) domain located near its C terminus but shares no significant with members family. A Northern blot analysis indicates expressed...
Abstract We have developed NetPath as a resource of curated human signaling pathways. As an initial step, provides detailed maps number immune pathways, which include approximately 1,600 reactions annotated from the literature and more than 2,800 instances transcriptionally regulated genes - all linked to over 5,500 published articles. anticipate become consolidated for pathways that should enable systems biology approaches.
Light and electron microscope immunocytochemistry with a monoclonal antibody against the N-terminal domain of human protein was used to determine cellular subcellular localization serotonin 5-HT2A receptors in central nervous system adult rat. Following immunoperoxidase or silver-intensified immunogold labeling, neuronal, somatodendritic, and/or axonal immunoreactivity detected numerous brain regions, including all those which ligand binding sites mRNA had previously been reported. The...
Although it is well established that reactive oxygen intermediates mediate the NF-κB activation induced by most agents, how H2O2 activates this transcription factor not understood. We found treatment of human myeloid KBM-5 cells with activated in a dose- and time-dependent manner much as tumor necrosis (TNF) did but unlike TNF, had no effect on IκBα degradation. Unexpectedly, however, like TNF-induced activation, H2O2-induced was blocked calpain inhibitor N-Ac-Leu-Leu-norleucinal, suggesting...
Abstract Increased expression of proinflammatory and proangiogenic factors are associated with aggressive tumor growth decreased survival patients head neck squamous cell carcinoma (HNSCC). In as much genes that regulated by nuclear factor NF‐κB suppress apoptosis, induce proliferation, mediate inflammation, angiogenesis metastasis, agents activation have potential treatment for various cancers including HNSCC. We demonstrate all HNSCC lines expressed constitutively active IκBα kinase (IKK),...
Tumor endothelial marker (TEM)8 was uncovered as a gene expressed predominantly in tumor endothelium, and its protein product recently identified the receptor for anthrax toxin. Here, we demonstrate that TEM8 is preferentially cells of neoplastic tissue. We used extracellular domain to search ligands alpha 3 subunit collagen VI an interacting partner. The TEM8-interacting region on 3(VI) mapped COOH-terminal C5 domain. Remarkably, also endothelium pattern concordant with TEM8. These results...
Because of the critical role nuclear transcription factor NF-kappaB in inflammation, viral replication, carcinogenesis, antiapoptosis, invasion, and metastasis, specific inhibitors this are being sought tested as treatments. activation is known to require p65 phosphorylation at serine residues 276, 529, 536 before it undergoes translocation. Small protein domains, termed transduction domains (PTDs), which able penetrate cell membranes can be used transport other proteins across membrane. We...
Atrial natriuretic peptide (ANP) binds to a transmembrane receptor having intrinsic guanylyl cyclase activity; this has been designated GC-A.Binding of ANP GC-A stimulates its catalytic activity, resulting in increased production the second messenger, cyclic GMP.Here we show that can be expressed insect cells using recombinant baculovirus and protein retained abilities bind function as an ANP-activated cyclase.In addition, produced was absolutely dependent on presence adenine nucleotides for...
The potent diuretic and natriuretic peptide hormone atrial factor (ANF), with vasodilatory activity also stimulates steroidogenic responsiveness in Leydig cells. actions of ANF are mediated by its interaction specific cell surface receptors the membrane-bound form guanylate cyclase represents an receptor (ANF-R). To understand mechanism action testicular steroidogenesis to identify cyclase/ANF-R that is expressed cells, primary structure murine has been deduced from cDNA sequence. A library...
Abstract Viral replication, immune regulation, and induction of various inflammatory growth-regulatory genes require activation a nuclear transcription factor (NF)-kappa B. Agents that can block NF-kappa B have potential to downstream responses mediated through this factor. Capsaicin (8-methyl-N-vanillyl-6-nonenamide) is quinone has been shown regulate wide variety activities activation. In the present study, we examined effect capsaicin its analogue, resiniferatoxin, on induced by different...
Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide. In this study, our objective was to identify differentially regulated proteins in HCC through a quantitative proteomic approach using iTRAQ. More than 600 were quantitated of which 59 overexpressed and 92 underexpressed as compared adjacent normal tissue. Several expressed not implicated previously HCC. A subset these (six each from upregulated downregulated groups) further validated immunoblotting immunohistochemical...
Chronic diseases, including inflammatory bowel disease (IBD) urgently need new biomarkers as a significant proportion of patients, do not respond to current medications. Inflammation is common factor in these and microbial sensing the intestinal tract critical initiate inflammation. We have identified ELMO1 (engulfment cell motility protein 1) sensor epithelial phagocytic cells that turns on signals. Using stem cell-based 'gut-in-a-dish' coculture model, we studied interactions between...
Abstract Expression patterns of 5‐HT 2A , 2B and 2C receptors during mouse embryogenesis were investigated using highly specific monoclonal antibodies. Differential overlapping spatio‐temporal receptor immunoreactivity observed active phases morphogenesis a variety embryonic tissues, including neuroepithelia brain spinal cord, notochord, somites, cranial neural crest, craniofacial mesenchyme epithelia, heart myocardium endocardial cushions, tooth germs, whisker follicles, cartilage striated...
Previously we have shown that treatment of ML-1a cells with TNF in the presence cycloheximide triggers apoptosis within 90 min. In present report used this system to investigate role ceramide action. We found while maximum DNA fragmentation response was induced by 1 nM TNF, synthetic membrane-permeable C-2, C-6, and C-8 ceramides had no effect even up 40 microM concentration. To roles action, ((2-isopropyl-1-(4-[3-N-methyl-N-(3,4-dimethoxy-phenethyl)amino]...
Gastric cancer is the second leading cause of death worldwide, both in men and women. A genomewide gene expression analysis was carried out to identify differentially expressed genes gastric adenocarcinoma tissues as compared adjacent normal tissues. We used Agilent's whole human genome oligonucleotide microarray platform representing ~41,000 carry analysis. Two-color employed directly compare between tumor Through this approach, we identified several previously known candidate along with a...
Serotonin (5-hydroxytryptamine, 5-HT) mediates many functions of the central and peripheral nervous systems by its interaction with specific neuronal glial receptors. Fourteen serotonin receptors belonging to seven families have been identified through physiological, pharmacological, molecular cloning studies. Monoclonal antibodies (MAbs) for each these receptor subtypes are needed characterize their expression, distribution, function in embryonic, adult, pathological tissues. In this...