Caffeic acid phenethyl ester (CAPE), an active component of propolis from honeybee hives, is known to have antimitogenic, anticarcinogenic, antiinflammatory, and immunomodulatory properties. The molecular basis for these diverse properties not known. Since the role nuclear factor NF-kappa B in responses has been documented, we examined effect CAPE on this transcription factor. Our results show that activation by tumor necrosis (TNF) completely blocked a dose- time-dependent manner. Besides...

Abstract Viral replication, immune regulation, and induction of various inflammatory growth-regulatory genes require activation a nuclear transcription factor (NF)-kappa B. Agents that can block NF-kappa B have potential to downstream responses mediated through this factor. Capsaicin (8-methyl-N-vanillyl-6-nonenamide) is quinone has been shown regulate wide variety activities activation. In the present study, we examined effect capsaicin its analogue, resiniferatoxin, on induced by different...

Abstract Because they have distinct intracellular domains, it has been proposed that the p60 and p80 forms of TNF receptor mediate different signals. Several signaling proteins isolated associate with either or receptor. By using muteins specific to receptors, we previously shown cytotoxicity nuclear factor-κB (NF-κB) activation are mediated through form endogenous What signals is less clear. This study was an effort answer question. HeLa cells, which express only were transfected cDNA then...

The efficacy of methotrexate (MTX), a widely used chemotherapeutic drug, is limited by its gastrointestinal toxicity and the mechanism which not clear. present study investigates possible role mitochondrial damage in MTX-induced enteritis. Small intestinal injury was induced Wistar rats administration 7 mg kg −1 body wt. MTX intraperitoneally for 3 consecutive days. resulted severe small extensive to enterocyte mitochondria. Respiratory control ratio, single most useful reliable test...

Generation of Fanconi anemia (FA) patient-specific induced pluripotent stem cells (iPSCs) has been reported to be technically challenging due the defects in FA-pathway patients' somatic cells. By inducible complementation FA-pathway, we successfully reprogrammed fibroblasts an FA patient iPSCs. CSCR19i-indCFANCA, one iPSC lines generated by was extensively characterized for its pluripotency and karyotype. In absence doxycycline (DOX) FANCA expression, this line showed cellular phenotypes FA,...

Abstract Background: One of the major toxic side effects methotrexate (MTX) is enterocolitis, for which there no efficient standard treatment. Nitric oxide overproduction has been reported to play an important role in MTX-induced mucositis. This study was designed investigate whether pretreatment with aminoguanidine (AG) – a selective iNOS inhibitor prevents mucositis rats. Methods: Rats were pretreated AG (30 and 50 mg/kg body weight) i.p. daily 1 h before MTX (7 administration 3...

The efficacy of methotrexate (MTX), a commonly used chemotherapeutic drug, is limited by intestinal injury. As the mechanism MTX-induced small injury not clear, there no definitive treatment for gastrointestinal present study investigates role mitochondrial apoptotic pathway in and examines whether aminoguanidine effective preventing damage. Eight Wistar rats were administered 3 consecutive i.p. injections 7 mg/kg body wt. MTX. Some pretreated with 30 mg or 50 (n = 6 each group). Protein...

Plant peptides have gained attention in the medicinal field due to their high anti-microbial and anti-cancer properties. Various plant sources are being used extract proteins be as a cure for variety of diseases. The latest studies show that effective treatment cancer ability preferentially bind receptors or membranes cells leading tumour growth inhibition, cytotoxicity, decreased proliferation, apoptosis. get internalized into causing cell agglutination aggregation. In addition acting...

Gestational diabetes mellitus (GDM) is a metabolic disturbance in pregnancy, traditionally defined as glucose intolerance with onset or first recognition during pregnancy the outcome of perinatal and offspring complications. The common diagnostic criteria oral tolerance test second third trimester pregnant women help to detect later complications GDM. Early detection lacking highly essential prevent We aim assess role glycated hemoglobin hematocrit for early screening GDM.We recruited 403...

Nitric Oxide (NO) where produced by endothelial nitric oxide synthase (eNOS) enzyme which are inhibited C-reactive protein (CRP) causes dysfunction and cardiovascular events. In the current study, we evaluated association of NO with hs-CRP in subjects coronary heart disease. This Case-Control study was conducted 60 CHD patients healthy controls age group 30 to 55 years at SRM Medical College Hospital Research Centre on attending Cardiology medicine OP. Blood samples were collected after...

Metformin, a widely used antihyperglycemic drug for the treatment of type 2 diabetes mellitus. The usage this therapeutic has become more common as frequency mellitus metabolic disorder is high worldwide especially in India. Improper disposal expired creating huge impact environment well financial prudence country. UV-visible spectrophotometry one simple and accurate method apposite validation drug. Our aim to evaluate compare extant metformin by spectrophotometry. We observed absorption...

Introduction: LDL-C undergoes modification to form Oxidized LDL a crucial occurrence in the oxidation hypothesis of atherogenesis.Oxidized and RLP-C stimulates immune inflammatory reactions promotes atherosclerosis.Because its lesser size along with high cholesterol content, increased residence period blood remnant lipoproteins are highly atherogenic.Remnant enter into arterial wall easily taken up directly by macrophages lead formation foam cells, thus initiating lipid-laden plaque.Oxidized...