A5007779722- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Nitric Oxide and Endothelin Effects
- Caveolin-1 and cellular processes
- Studies on Chitinases and Chitosanases
- Traumatic Brain Injury and Neurovascular Disturbances
- T-cell and B-cell Immunology
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Graphene and Nanomaterials Applications
- Carbon and Quantum Dots Applications
- Cerebrovascular and genetic disorders
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Barrier Structure and Function Studies
- Microbial Inactivation Methods
- Neurogenesis and neuroplasticity mechanisms
- Ferroptosis and cancer prognosis
- Signaling Pathways in Disease
- Advanced Glycation End Products research
- Neurological Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2022-2025
Institute of Neurobiology
2024
Massachusetts General Hospital
2018-2023
Harvard University
2018-2023
Polish Academy of Sciences
2022-2023
Marion General Hospital
2022
MGH Institute of Health Professions
2022
Boston University
2022
Munich Cluster for Systems Neurology
2015-2016
Institut für Urheber- und Medienrecht
2016
Abstract Immune checkpoint blockers (ICBs) have failed in all phase III glioblastoma (GBM) trials. Here, we show that regulatory T (Treg) cells play a key role GBM resistance to ICBs experimental gliomas. Targeting glucocorticoid-induced TNFR-related receptor (GITR) Treg using an agonistic antibody (αGITR) promotes CD4 cell differentiation into effector cells, alleviates cell-mediated suppression of anti-tumor immune response, and induces potent GBM. The reprogrammed GBM-infiltrating express...
Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), the most common inherited small-vessel disease, is associated vascular aggregation of mutant Notch3 protein, dysfunction cerebral vessels, dementia. Pericytes, perivascular cells involved in microvascular function, express Notch3. Therefore, we hypothesize that these may play a role pathogenesis CADASIL.Two-, 7-, 12-month-old CADASIL mice (TgNotch3(R169C) ) wild-type controls were examined...
Abstract Real‐time X‐ray or magnetic resonance imaging are known methods used for biomedical diagnosis. By the oral administration of barium meal, can be extended use in soft tissue imaging. The ingestion a fluorescent probe is new approach to living species. Here, water‐soluble carbon nano‐onions introduced as nontoxic, reagent enabling Drosophila melanogaster (fruit flies) imaged alive. It demonstrated that these nano‐onions, synthesized from wood waste, colorfully image all development...
Immune checkpoint blockers (ICBs) have failed in all phase III glioblastoma trials. Here, we found that ICBs induce cerebral edema some patients and mice with glioblastoma. Through single-cell RNA sequencing, intravital imaging, CD8 + T cell blocking studies mice, demonstrated this results from an inflammatory response following antiprogrammed death 1 (PD1) antibody treatment disrupts the blood–tumor barrier. Used lieu of immunosuppressive corticosteroids, angiotensin receptor blocker...
Carbon nano-onions, as a newly-emergent member of the carbon family, have attracted attention in its both dry and wet applications. Here we report vivo effects water-soluble nano-onions (wsCNOs) introduced common food web two model organisms: unicellular Escherichia coli (E. coli) multicellular Caenorhabditis elegans (C. elegans). At first, CNOs are fed to E. and, subsequently, C. elegans. It is found that wsCNOs serve highly-fluorescent bioimaging agent. The results do not reflect any toxic...
Aging leads to a gradual decline in the fidelity of cerebral blood flow (CBF) responses neuronal activation, resulting an increased risk for stroke and dementia. However, it is currently unknown when age-related cerebrovascular dysfunction starts or which vascular components functions are first affected. The aim this study was examine function microcirculation throughout aging mice. Microcirculation challenged by inhalation 5% 10% CO2 forepaw stimulation 6-week, 8-month, 12-month-old FVB/N...
Water soluble fluorescent carbon nano onions (wsCNO) cross the blood brain barrier (BBB) in CADASIL murine model as well GBM induced mice.
Arginase-1 is an important component of the intricate mechanism regulating arginine availability during immune responses and nitric oxide synthase (NOS) activity. In this study Arg1(fl/fl)/Tie2-Cre(tg/-) mice were developed to investigate effect arginase-1 related depletion on NOS2- NOS3-dependent NO production jejunal microcirculation under resting endotoxemic conditions, in lacking endothelial hematopoietic cells.Arginase-1-deficient as compared with control exhibited higher plasma...
Abstract Background Immune checkpoint inhibitors (ICIs) present clinical benefits in many cancer patients but invariably fail glioblastoma (GBM), the most common and deadly primary brain tumor. The lack of ICIs efficacy GBM is attributed to accumulation tumor-reprogrammed glioma-associated myeloid cells (GAMs) that create a “cold” immunosuppressive tumor microenvironment (TME), impeding infiltration activation effector T cells. GBM-derived αvβ3/αvβ5-integrin ligands, including SPP1, were...
To study the role and (sub) cellular nitric oxide (NO) constitution in various disease processes, its direct specific detection living cells tissues is a major requirement. Several methods are available to measure oxidation products of NO, but NO itself has proved challenging. We visualized production using NO-sensitive copper-based fluorescent probe (Cu 2FL2E) two-photon laser scanning microscopy (TPLSM). Cu 2FL2E demonstrated high sensitivity specificity for synthesis, combined with low...
ABSTRACT Immune checkpoint inhibitors (ICI) presented clinical benefits in many cancer patients but invariably fail glioblastoma (GBM), the most common and deadly primary brain tumor. Lack of ICI efficacy GBM is attributed to accumulation immunosuppressive myeloid cells that create “cold” tumor microenvironment (TME) impeding infiltration activation effector T cells. We developed a designer RGD peptide hindered glioma-instigated, integrin-mediated pro-tumoral reprogramming blocked...
This research paper represents the theoretical and experimental study of intra organelle nano poration multi layer osteoblast cell .It is observed that Pico pulse has remarkable response for pores generation on chemicals are entered into cell.The reported encourage efficiency nanoporation can be control by specification micro chip.It also exposed key parameter such as voltage, pore radius, density, pressure, surface tension ion uptake externally controlled user defined hybrid 3D chip.
<title>Abstract</title> <bold>Background</bold> Immune checkpoint inhibitors (ICIs) present clinical benefits in many cancer patients but invariably fail glioblastoma (GBM), the most common and deadly primary brain tumor. The lack of ICIs efficacy GBM is attributed to accumulation tumor-reprogrammed myeloid cells (GAMs) that create “cold” immunosuppressive tumor microenvironment (TME), impeding infiltration activation effector T cells. GBM-derived αvβ3/αvβ5-integrin ligands, including SPP1,...
Abstract Astrocytes comprise ∼50% of all brain cells and present distinct morphological, molecular functional properties in different regions. In glioblastoma (GBM), an aggressive primary tumour, tumour-associated astrocytes (TAAs) become activated exhibit transcriptomic profiles, morphology functions supporting disease progression. Heterogeneity specific roles TAAs within various regions tumours are poorly known. Advancements single-cell spatial transcriptomics allow to profile at...
Abstract BACKGROUND Glioblastoma (GBM) is a highly diffusive tumor which restricts the efficacy of surgical resection and facilitates recurrence. Matrix metalloproteinase 2 (MMP2) extracellular matrix protease that activated by MMP14 expressed microglia plays central role in tissue remodeling progression. The R132H mutation isocitrate dehydrogenase 1 (IDH1R132H) commonly observed gliomas, results hypermethylator phenotype GBMs associated with better survival. impacts IDH1R132H on...
<title>Abstract</title> Chitinase-3-like protein 1 (CHI3L1) is a secreted, non-enzymatic glycoprotein that interacts with cell-surface and extracellular-matrix proteins, proteoglycans, polysaccharides. Many studies reported the overexpression of CHI3L1 in various cancers, but its exact role tumorigenesis/cancer progression remains elusive. We performed comprehensive analysis <italic>CHI3L1</italic> expression public repositories including single-cell RNAseq datasets to determine cellular...
Abstract Clinical trials with immune checkpoint inhibitors (ICI) have benefited many cancer patients but failed in a number of tumors, including glioblastoma (GBM), the most common and aggressive primary brain tumor adults. The main obstacle for ICI efficacy GBM is continuously evolving microenvironment (TME), which antitumor immunity inhibited or eluded by tumor-secreted factors. Accumulation reprogramming myeloid cells (GAMs) creates “cold” immunosuppressive TME poor infiltration...
Abstract Glioblastoma (GBM) shows high level of resistance to currently available treatments including the standard care and immunotherapy, representing most fatal cancer type. Our study revealed that immune suppression by regulatory T cells (Treg) secondary therapy with checkpoint blocker (anti-PD1) confers this resistance. In GBM tumor microenvironment, Treg increased suppressive phenotype were found which frequency anergic increase after ICB therapy, potentially contributing Targeting has...