The hypoxia-inducible factors (HIF) are transcription that activate the adaptive hypoxic response when oxygen levels low. HIF transcriptional program increases delivery by inducing angiogenesis and promoting metabolic reprograming favors glycolysis. two major HIFs, HIF-1 HIF-2, mediate this during prolonged hypoxia in an overlapping sequential fashion is referred to as switch. Both proteins consist of unstable alpha chain a stable beta chain. instability chains mediated prolyl hydroxylase...

It is acknowledged that cancer cells are able to undergo senescence in response clinically used chemotherapeutics. Moreover, recent years have provided evidence some drugs can selectively remove senescent cells. Therefore, it essential properly identify and characterize cells, especially when comes cancer. Senescence was induced various cell lines (A549, SH-SY-5Y, HCT116, MDA-MB-231, MCF-7) following treatment with doxorubicin, irinotecan, methotrexate, 5-fluorouracil, oxaliplatin, or...

Bioenergetic failure, oxidative stress, and changes in mitochondrial morphology are common pathologic hallmarks of amyotrophic lateral sclerosis (ALS) several cellular animal models. Disturbed physiology has serious consequences for proper functioning the cell, leading to chronic stress. Mitochondria, being center metabolism, play a pivotal role adaptation stress conditions. We found that dysfunction processes differ primary fibroblasts derived from patients diagnosed with either sporadic or...

We analyzed the effects of selective knockdown either HIF-1α or HIF-2α on transcriptional response to hypoxia human umbilical endothelial cells at two time-points (2 h and 8 h) hypoxia. focused 13 previously identified hypoxia-responsive genes, pre-selected have different activation kinetics proportions HRE motifs annotated HIF-1 HIF-2 in open promoters - chromatin DNase-hypersensitive sites (DHS) regions within ±1 kb gene start. report that genes activated by both 2 tend be earlier than...

Chronic myeloid leukemia (CML) cells circulate between blood and bone marrow niche, representing different microenvironments. We studied the role of two RNA-binding proteins, T-cell-restricted intracellular antigen (TIAR), fragile X mental retardation protein (FMRP) in regulation translation CML residing settings mimicking peripheral microenvironment (PBM) (BMM). The outcomes showed how conditions shaped process through TIAR FMRP activity, considering its relevance therapy resistance....

As suggested by the origin of word, sphingolipids are mysterious molecules with various roles in antagonistic cellular processes such as autophagy, apoptosis, proliferation and differentiation. Moreover, have recently been recognized important messengers signaling pathways. Notably, sphingolipid metabolism disorders observed pathological conditions cancer neurodegeneration.The existing formal models focus mainly on de novo ceramide synthesis or limited to biochemical transformations...

Aging-related biochemical changes in nerve cells lead to dysfunctional synapses and disrupted neuronal circuits, ultimately affecting vital processes such as brain plasticity, learning, memory. The imbalance between excitation inhibition synaptic function during aging contributes cognitive impairment, emphasizing the importance of compensatory mechanisms. Fear conditioning-related plasticity somatosensory barrel cortex, relying on proper functioning extensive up regulation GABAergic system,...

In this paper, we investigate efficient estimation of differential entropy for multivariate random variables. We propose bias correction the nearest neighbor estimator, which yields more accurate results in higher dimensions. order to demonstrate accuracy improvement, calculated corrected estimator several families For distributions, considered case independent marginals and dependence structure between marginal distributions described by Gaussian copula. The presented solution may be...

Molecular synaptic aging perturbs neurotransmission and decreases the potential for neuroplasticity. The direction degree of changes observed in are often region or cell specific, hampering generalization age-related effects. Using real-time PCR Western blot analyses, we investigated several presynaptic markers (Vglut1, Vglut2, Gad65, Gad67, Vgat, synaptophysin) involved initial steps glutamatergic GABAergic neurotransmission, cortical regions, young (3–4 months old), middle-aged (1 year old...

AMA Charzyńska A, Nałęcz Rybiński M, Gambin A. RESEARCH PAPERSensitivity analysis of mathematical models signaling pathways. BioTechnologia. 2012;93(3):291-308. doi:10.5114/bta.2012.46584. APA Charzyńska, A., Nałęcz, Rybiński, M., & Gambin, (2012). BioTechnologia, 93(3), 291-308. https://doi.org/10.5114/bta.2012.46584 Chicago Agata, Anna Mikołaj and Gambin. 2012. "RESEARCH pathways". BioTechnologia 93 (3): Harvard pp.291-308. MLA Agata et al. pathways." vol. 93, no. 3, 2012, pp. Vancouver

Chondroitin sulfate proteoglycans (CSPGs) consist of core proteins and glycosaminoglycan side chains. Tenascins, hyaluronan proteoglycan link protein 1 (HAPLN), CSPGs with a backbone to constitute perineuronal nets (PNNs), which ensheath preferentially highly active neurons maintain architecture stabilize synapses, but restrict repair plasticity. Spinal cord injury increases CSPG levels in the lesion proximity, limiting permissiveness extracellular milieu for fiber regrowth, however...

Most cases of Parkinson's disease (PD) are idiopathic, with unknown aetiology and genetic background. However, approximately 10 % caused by defined mutations, among which mutations in the parkin gene most common. There is increasing evidence involvement mitochondrial dysfunction development both idiopathic PD. data on changes reported different studies inconsistent, can reflect variability background disease. Mitochondria, as a plastic dynamic organelles, first place cell to respond external...

Abstract Motivation: Stochasticity is an indispensable aspect of biochemical processes at the cellular level. Studies on how noise enters and propagates in systems provided us with non-trivial insights into origins stochasticity, total, however, they constitute a patchwork different theoretical analyses. Results: Here we present flexible widely applicable decomposition tool that allows to calculate contributions individual reactions total variability system’s output. With package it is,...

<title>Abstract</title> Aging-related biochemical changes in nerve cells lead to dysfunctional synapses and disrupted neuronal circuits, ultimately affecting vital processes such as brain plasticity, learning, memory. The imbalance between excitation inhibition synaptic function during aging contributes cognitive impairment, emphasizing the importance of compensatory mechanisms. Fear conditioning-related plasticity somatosensory barrel cortex, relying on proper functioning extensive...

Background: Sphingolipids - as suggested by the prefix in their name are mysterious molecules, which play surprisingly various roles opposable cellular processes, like autophagy, apoptosis, proliferation and differentiation. Recently they have been also recognized important messengers signalling pathways. More importantly, sphingolipid metabolism disorders were observed pathological conditions such cancer neurodegeneration. Results: Existing formal models of sphingolipids concentrates mostly...

Abstract The hypoxia-inducible factors (HIF) are transcription that activate the adaptive hypoxic response when oxygen levels low. HIF transcriptional program increases delivery by inducing angiogenesis and promoting metabolic reprograming favors glycolysis. two major HIFs, HIF-1 HIF-2, mediate this during prolonged hypoxia in an overlapping sequential fashion is referred to as switch. Both proteins consist of unstable alpha chain a stable beta chain. instability chains mediated prolyl...

Stochasticity is an indispensable aspect of biochemical processes at the cellular level. Studies on how noise enters and propagates in systems provided us with nontrivial insights into origins stochasticity, total however they constitute a patchwork different theoretical analyses. Here we present flexible generally applicable decomposition tool, that allows to calculate contributions individual reactions variability system's output. With package it therefore possible quantify systems. We...

Chronic myeloid leukemia (CML) cells circulate between blood and bone marrow niche, representing different microenvironments. We studied the role of two RNA-binding proteins, T-cell-restricted intracellular antigen (TIAR) fragile X mental retardation protein (FMRP) in regulation translation CML residing settings mimicking peripheral microenvironment (PBM) (BMM). The outcomes showed how conditions shaped process through TIAR FMRP activity, considering its relevance therapy resistance. QuaNCAT...