A5007960180- Ion Channels and Receptors
- Genetic and Kidney Cyst Diseases
- Biomedical Research and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Microbial Metabolic Engineering and Bioproduction
- Herbal Medicine Research Studies
- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- Ion channel regulation and function
- Congenital heart defects research
- Calcium signaling and nucleotide metabolism
- Hearing, Cochlea, Tinnitus, Genetics
- Hedgehog Signaling Pathway Studies
- Plant Stress Responses and Tolerance
- Parasites and Host Interactions
- Cellular transport and secretion
- Biotin and Related Studies
- Cancer Immunotherapy and Biomarkers
- Zebrafish Biomedical Research Applications
- bioluminescence and chemiluminescence research
- Connexins and lens biology
- Genetics, Aging, and Longevity in Model Organisms
International Institute of Molecular and Cell Biology
2013-2025
University Medical Center Freiburg
2006-2009
Friedrich Miescher Institute
2001
University of Warsaw
1998
The primary cilium has evolved as a multifunctional cellular compartment that decorates most vertebrate cells. Cilia sense mechanical stimuli in various organs, but the molecular mechanisms convert deflection of cilia into intracellular calcium transients have remained elusive. Polycystin-2 (TRPP2), an ion channel mutated polycystic kidney disease, is required for cilia-mediated lacks mechanosensitive properties. We find here TRPP2 utilizes TRPV4 to form mechano- and thermosensitive sensor...
Src family tyrosine kinases (SFKs) regulate the function of several transient receptor potential (TRP) members, yet their role in regulation vanilloid subfamily member 4 protein (TRPV4) remains controversial. TRPV4 is a calcium-permeable channel activated by numerous physical and chemical stimuli. Here we show that SFKs mediate phosphorylation different cell lines. Using mass spectrometric analysis, identified two novel sites cytosolic N- C-terminal tails TRPV4. Substitution either with...
Thalamocortical loops have been implicated in the control of higher-order cognitive functions, but advances our understanding molecular underpinnings neocortical organization not accompanied by similar analyses thalamus. Using expression-based correlation maps and manual mapping mouse human datasets available Allen Brain Atlas, we identified a few individual regions several sets molecularly related nuclei that partially overlap with classic grouping is based on topographical localization...
Maturation of 18S rRNA and biogenesis the 40S ribosomes in yeast requires a large number trans-acting factors, including U3 small nucleolar ribonucleoprotein (U3 snoRNP), recently characterized cyclase-like protein Rcl1p. snoRNP is key particle orchestrating early 35S cleavage events. A unique property Rcl1p that it specifically associates with snoRNP, but this association appears to occur only at level nascent not monoparticle. Here we report characterization Bms1p, multiple structures,...
Innate immunity is the first line of host defense against pathogens. Here, through global transcriptome and proteome analyses, we uncover that newly described cytoplasmic poly(A) polymerase TENT-5 (terminal nucleotidyltransferase 5) enhances expression secreted innate effector proteins in Caenorhabditis elegans . Direct RNA sequencing revealed multiple mRNAs with signal peptide–encoding sequences have shorter tails tent-5 –deficient worms. Those are translated at endoplasmic reticulum where...
Planar cell polarity signaling controls a variety of polarized behaviors. In multiciliated Xenopus epidermal cells, recruitment Dishevelled (Dvl) to the basal body and its localization center ciliary rootlet are required correctly position motile cilia. We now report that anaphase-promoting complex (APC/C) recognizes D-box motif Dvl ubiquitylates on highly conserved lysine residue. Inhibition APC/C function by knockdown ANAPC2 subunit disrupts cilia alters directionality fluid movement along...
Autosomal dominant polycystic liver disease (PCLD) is caused by mutations of either PRKCSH or Sec63, two proteins associated with the endoplasmic reticulum (ER). Both are involved in carbohydrate processing, folding and translocation newly synthesized glycoproteins. It postulated that defective quality control initiates reticulum-associated degradation (ERAD), which disrupts hepatic homeostasis patients Sec63 mutations. However, precise molecular mechanisms not known. Here, we show...
Clathrin-mediated endocytosis (CME) is a process in which ligands and their corresponding receptors at the cell surface are internalized via clathrin-coated invaginations of plasma membrane. Both clathrin endocytic cargo recruited to pit by adaptor protein complex AP2. AP2 resides cytoplasm closed conformation opens upon interacting with Effective CME requires both pools AP2, open closed, effective transition between these states but mechanisms regulating this only partially understood....
Abstract Immunotherapy revolutionized cancer treatment in the last decade. Both natural killer (NK) cells and T are key components of host immunity against malignant that being extensively investigated field immunotherapy. While approaches have been developed to improve antitumor activity NK cells, tumor microenvironment remains an obstacle effective cell-based therapies. Here, we demonstrated cancer-conditioned medium suppresses cells. Ammonia, a by-product cell metabolism, accumulated...
Familial Alzheimer's disease (AD) is caused by mutations in the genes that encode amyloid precursor protein (APP) and presenilins. Disturbances calcium homeostasis have been observed various cellular animal models of AD are proposed to underlie pathogenesis disease. Furthermore, wildtype presenilins were shown regulate endoplasmic reticulum (ER) homeostasis, although their precise mechanism action remains controversial. To investigate whether APP also affects ER levels, we used RNA...
Saccharomyces cerevisiae nuclear genes SUV3 and DSS1 encode putative RNA helicase RNase II, respectively, which are subunits of the mitochondrial degradosome (mtEXO): a three-protein complex has 3' to 5' exoribonuclease activity plays major role in regulating stability RNA. Lack either two gene products results respiratory negative phenotype, while on molecular level it causes total block translation, loss vitro changes processing many mtRNAs. We have found that yeast PET127 present low or...
Familial Alzheimer's disease (FAD)-causing mutations in presenilins were shown to alter intracellular calcium dynamics, including store-operated entry (SOCE). However, the involvement of FAD-linked amyloid precursor protein (APP) SOCE remains controversial. Here, we used gain-of-function and loss-of-function approaches shed light on this issue. We found that Jurkat cells, which exhibit prominent mediated by Orai channels, maintain low APP levels. The ectopic expression APP, either with...
Abstract Calcium is involved in vision processes the retina and implicated various pathologies, including glaucoma. Rod cells rely on store-operated calcium entry (SOCE) to safeguard against prolonged lowering of intracellular ion concentrations. Zebrafish that lacked endoplasmic reticulum Ca 2+ sensor Stim2 ( stim2 knockout [KO]) exhibited impaired lower light perception-related gene expression. We sought understand mechanisms are responsible for impairment KO zebrafish. The single-cell RNA...