A5047068703- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Machine Learning in Materials Science
- Microbial Natural Products and Biosynthesis
- Advanced biosensing and bioanalysis techniques
- Genetics, Aging, and Longevity in Model Organisms
- Gene expression and cancer classification
- RNA Research and Splicing
- Parasites and Host Interactions
- RNA modifications and cancer
- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Parasitic Diseases Research and Treatment
International Institute of Molecular and Cell Biology
2021-2025
Innate immunity is the first line of host defense against pathogens. Here, through global transcriptome and proteome analyses, we uncover that newly described cytoplasmic poly(A) polymerase TENT-5 (terminal nucleotidyltransferase 5) enhances expression secreted innate effector proteins in Caenorhabditis elegans . Direct RNA sequencing revealed multiple mRNAs with signal peptide–encoding sequences have shorter tails tent-5 –deficient worms. Those are translated at endoplasmic reticulum where...
Ribonucleic acids (RNAs) play crucial roles in living organisms and some of them, such as bacterial ribosomes precursor messenger RNA, are targets small molecule drugs, whereas others, e.g. riboswitches or viral RNA motifs considered potential therapeutic targets. Thus, the continuous discovery new functional increases demand for developing compounds targeting them methods analyzing RNA-small interactions. We recently developed fingeRNAt-a software detecting non-covalent bonds formed within...
Abstract Stability and translation of mRNAs, both endogenous therapeutic, is determined by poly(A) tail. Direct RNA sequencing enables single-molecule measurements lengths, avoiding amplification bias. It also holds potential for observation non-adenosines within poly(A), known to influence mRNA fate. However, there no computational method detect composite tails in Sequencing data. To address this gap, we introduce the Ninetails, a neural network-based tool that accurately identifies...
Computational methods play a pivotal role in drug discovery and are widely applied virtual screening, structure optimization, compound activity profiling. Over the last decades, almost all attention medicinal chemistry has been directed to protein-ligand binding, computational tools have created with this target mind. With novel discoveries of functional RNAs their possible applications, gained considerable as potential targets. However, availability bioinformatics for nucleic acids is...
Abstract Polyadenylation is an important post-transcriptional process that governs mRNA stability and expression. Advancements in direct RNA sequencing recent years have clarified many aspects of this intricate regulation, revealing the influence various factors. Here, we used Nanopore Direct Sequencing to investigate association between genome-wide poly(A) tail profiles sexual dimorphism Caenorhabditis elegans . Our results demonstrate sex-dependent differences both gene expression...
ABSTRACT Ribonucleic acids (RNA) play crucial roles in living organisms as they are involved key processes necessary for proper cell functioning. Some RNA molecules, such bacterial ribosomes and precursor messenger RNA, targets of small molecule drugs, while others, e.g., riboswitches or viral motifs considered potential therapeutic targets. Thus, the continuous discovery new functional increases demand developing compounds targeting them methods analyzing RNA—small interactions. We recently...
Abstract The nematode Caenorhabditis elegans is one of the best-studied model organisms in molecular biology; however, many aspects its physiology and functions genes remain poorly understood. In this study, we investigated role nematode-specific NSPC proteins, whose mRNAs were recently identified as primary targets poly(A) polymerase TENT-5. Surprisingly, found that NSPCs are exclusively expressed excretory gland cell, a cell with still unclear functionality. Using an optogenetic approach,...
ABSTRACT Computational methods play a pivotal role in drug discovery and are widely applied virtual screening, structure optimization, compound activity profiling. Over the last decades, almost all attention medicinal chemistry has been directed to protein-ligand binding, computational tools have created with this target mind. With novel discoveries of functional RNAs their possible applications, gained considerable as potential targets. However, availability bioinformatics for nucleic acids...