A5087522846- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Eating Disorders and Behaviors
- Circular RNAs in diseases
- Genomics and Rare Diseases
- MicroRNA in disease regulation
- RNA Research and Splicing
- Pregnancy and preeclampsia studies
- Child Nutrition and Feeding Issues
- Mycobacterium research and diagnosis
- interferon and immune responses
- Berberine and alkaloids research
- Cancer-related gene regulation
- Adolescent and Pediatric Healthcare
- Tuberculosis Research and Epidemiology
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Biomedical Text Mining and Ontologies
- Ferroptosis and cancer prognosis
- Genetic Associations and Epidemiology
- Inflammasome and immune disorders
- Sodium Intake and Health
- Genomics, phytochemicals, and oxidative stress
Johns Hopkins University
2023-2025
Johns Hopkins All Children's Hospital
2023-2025
Sidney Kimmel Comprehensive Cancer Center
2023-2025
Kyoto University
2021-2024
Johns Hopkins Medicine
2023
RIKEN Center for Biosystems Dynamics Research
2022
NHS Grampian
2019-2021
University of Aberdeen
2019-2021
City Hospital
2021
Institute of Genomics and Integrative Biology
2013-2021
With the advent of next-generation sequencing, large-scale initiatives for mining whole genomes and exomes have been employed to better understand global or population-level genetic architecture. India encompasses more than 17% world population with extensive diversity, but is under-represented in sequencing datasets. This gave us impetus perform analyze genome 1029 healthy Indian individuals under pilot phase 'IndiGen' program. We generated a compendium 55,898,122 single allelic variants...
Recent studies suggest that long non-coding RNAs (lncRNAs) contribute to medulloblastoma (MB) formation and progression. We have identified an lncRNA, lnc-HLX-2-7, as a potential therapeutic target in group 3 (G3) MBs. lnc-HLX-2-7 RNA specifically accumulates the promoter region of HLX, sense-overlapping gene which activates HLX expression by recruiting multiple factors, including enhancer elements. sequencing chromatin immunoprecipitation reveal binds promoters several oncogenes, TBX2,...
31 Background: Prostate Cancer (PCa) is one of the leading causes cancer deaths among men worldwide. Although Prostate-Specific Antigen (PSA) test widely used for screening, several advisory groups recommend against PSA because its non-specific and suboptimal performance. Hence, there an urgent unmet need novel more accurate biomarker panels PCa detection. Methods: To develop alternate assay, we collected voided urine (50 ml) from 236 pre- 198 matched post-prostatectomy with PCa, 76 benign...
The field of epitranscriptomics is undergoing a technology-driven revolution. During past decades, RNA modifications like N6-methyladenosine (m6A), pseudouridine (ψ), and 5-methylcytosine (m5C) became acknowledged for playing critical roles in cellular processes. Direct sequencing by Oxford Nanopore Technologies (ONT) enabled the detection native RNA, detecting noncanonical nucleosides properties raw data. Consequently, field's cutting edge has heavy component computer science, opening new...
With a higher throughput and lower cost in sequencing, second generation sequencing technology has immense potential for translation into clinical practice the realization of pharmacogenomics based patient care. The systematic analysis whole genome sequences to assess variability pharmacokinetics pharmacodynamics responses towards drugs would be next step future medicine line with vision personalizing medicine.
The human mitochondrial genome has been reported to have a very high mutation rate as compared with the nuclear genome. A large number of mutations show significant phenotypic association and are involved in broad spectrum diseases. In recent years, there remarkable progress understanding genetics. availability next-generation sequencing (NGS) technologies not only reduced cost by orders magnitude but also provided us good quality sequences coverage, thereby enabling decoding this study, we...
Mycobacterium tuberculosis, along with closely related species, commonly known as M. tuberculosis complex (MTBC), causes in humans and other organisms. Tuberculosis is a disease high morbidity mortality, especially the third world. The genetic variability between clinical isolates of MTBC has been poorly understood, although recent years have seen re-sequencing large number from around availability genomic data multiple public domain would potentially offer unique opportunity toward...
Abstract Long noncoding RNA s (lnc s) are a recently discovered class of functional encoded by metazoan genomes. Recent studies suggest larger regulatory role for lnc in critical biological and disease processes. Mounting evidence on the regulating key processes immune system prompted us to hypothesize as regulators pathophysiology Sjögren's syndrome ( SS ). We used two similar approaches based reanalysis microarray expression datasets curation ‐protein coding gene interactions from...
One of the most common RNA modifications found in mammalian brain is N6-methyladenosine (m6A) and this modification has been implicated fine-tuning neuronal gene expression by regulating metabolism with consequent impact on function behaviour. The presence, absence, overall influence m6A any given transcript governed so-called machinery genes include 'writers', 'erasers' 'readers.' Changes functional pathophysiology major depression (MD) its consequence mediated regulation not explored. To...
Abstract Recent technological advances in sequencing DNA and RNA modifications using high-throughput platforms have generated vast epigenomic epitranscriptomic datasets whose power transforming life science is yet fully unleashed. Currently available silico methods facilitated the identification, positioning quantitative comparisons of individual modification sites. However, essential challenge to link specific ‘epi-marks’ gene expression particular context cellular biological processes...
Pregnancy is a special condition where many metabolic changes may occur because of increased requirement essential micronutrients such as iron and iodine. Foetal thyroid starts producing its own hormones after 12 weeks gestation. Therefore, the first trimester very crucial for meeting hormone requirements mother foetus. Iodine deficiency affect mental physical growth Hence, it important to establish programme on screening pregnant women dysfunction tests along with established status...
Background Evidence suggests that investing in specialist eating disorders services for young people with anorexia nervosa could have important implications the NHS, potential to improve health outcomes and reduce costs through reductions number length of hospital admissions. Objectives The primary objectives were evaluate cost-effectiveness alternative community-based models service provision model impact changes services. Design Observational surveillance study using Child Adolescent...
We describe here the draft genome sequence of Sporosarcina pasteurii, a urease-producing bacterium with potential applications in biocement production.
Abstract The prognosis of childhood medulloblastoma (MB) is often poor, and it usually requires aggressive therapy that adversely affects quality life. microRNA-211 (miR-211) was previously identified as an important regulator cells descend from neural cells. Since medulloblastomas primarily affect with similar ontogeny, we investigated the role mechanism miR-211 in MB. Here showed expression highly downregulated cell lines, PDXs, clinical samples different MB subgroups (SHH, Group 3, 4)...
Medulloblastoma, the most common malignant pediatric brain tumor, is classified into four main molecular subgroups, but group 3 and 4 tumors are difficult to subclassify have a poor prognosis. Rapid point-of-care diagnostic prognostic assays needed improve medulloblastoma risk stratification management. N6-methyladenosine (m6A) RNA modification long non-coding RNAs (lncRNAs) play central role in tumor progression, their impact on gene expression associated clinical outcomes unknown. Here we...
A large repertoire of gene-centric data has been generated in the field zebrafish biology. Although bulk these are available public domain, most them not readily accessible or nonstandard formats. One major challenge is to unify and integrate widely scattered sources. We tested hypothesis that active community participation could be a viable option address this challenge. present here our approach create standards for assimilation sharing information system open database intercommunication....
Aim: Numerous drugs are being widely prescribed for COVID-19 treatment without any direct evidence the drug safety/efficacy in patients across diverse ethnic populations. Materials & methods: We analyzed whole genomes of 1029 Indian individuals (IndiGen) to understand extent drug–gene (pharmacogenetic), drug–drugand drug–drug–gene interactions ;associated with therapy population. Results: identified 30 clinically significant pharmacogenetic variants and 73 predicted deleterious variants....
Evidence suggests specialist eating disorders services for children and adolescents with anorexia nervosa have the potential to improve outcomes reduce costs through reduced hospital admissions. This study aimed evaluate cost-effectiveness of assessment diagnosis in community-based child adolescent mental health (CAMHS) compared generic CAMHS nervosa. Observational, surveillance aged 8 17, contact UK or Republic Ireland a first episode Data were reported by clinicians at baseline, 6...
Abstract BACKGROUND Medulloblastoma (MB) is the predominant pediatric brain tumor, subdivided into four molecular subgroups, with group 3 and 4 tumors posing significant clinical challenges due to poor prognosis classification. This study investigates roles of N6-methyladenosine (m6A) long non-coding RNAs (lncRNAs) in MB, aiming develop diagnostic prognostic tools. METHODS We analyzed transcriptome from 1236 samples identify m6A-associated lncRNA signature (M6LSig). A multivariate-cox...
Abstract Recent studies suggest that long non-coding RNAs (lncRNAs) contribute to medulloblastoma formation and progression. We have identified a lncRNA, lnc-HLX-2-7, as potential therapeutic target in group 3 (G3) medulloblastomas. lnc-HLX-2-7 RNA specifically accumulates the promoter region of HLX, its host gene, which activates HLX expression by recruiting multiple factors, including enhancer elements. sequencing chromatin immunoprecipitation reveal binds promoters several oncogenes,...
<title>Abstract</title> Medulloblastoma, the most common malignant pediatric brain tumor, is classified into four main molecular subgroups, but group 3 and 4 tumors are difficult to subclassify have a poor prognosis. Rapid point-of-care diagnostic prognostic assays needed improve medulloblastoma risk stratification management. N6-methyladenosine (m6A) RNA modification long non-coding RNAs (lncRNAs) play central role in tumor progression, their impact on gene expression associated clinical...