Rahul C. Bhoyar
A5024069615- SARS-CoV-2 and COVID-19 Research
- Genomics and Rare Diseases
- Animal Virus Infections Studies
- Cardiac electrophysiology and arrhythmias
- Immunodeficiency and Autoimmune Disorders
- CRISPR and Genetic Engineering
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Pharmacogenetics and Drug Metabolism
- Blood groups and transfusion
- Vaccine Coverage and Hesitancy
- Computational Drug Discovery Methods
- COVID-19 epidemiological studies
- Plant Virus Research Studies
- Poxvirus research and outbreaks
- SARS-CoV-2 detection and testing
- Lipoproteins and Cardiovascular Health
- Chronic Lymphocytic Leukemia Research
- RNA regulation and disease
- Bacteriophages and microbial interactions
- BRCA gene mutations in cancer
- Hemoglobinopathies and Related Disorders
- Herpesvirus Infections and Treatments
- Viral Infections and Immunology Research
- vaccines and immunoinformatics approaches
Institute of Genomics and Integrative Biology
2020-2025
Council of Scientific and Industrial Research
2020-2021
Fortis Memorial Research Institute
2020
Academy of Scientific and Innovative Research
2020
Rashtrasant Tukadoji Maharaj Nagpur University
2015-2019
Karnataka Veterinary Animal and Fisheries Sciences University
2018
Delhi, the national capital of India, experienced multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreaks in 2020 and reached population seropositivity >50% by 2021. During April 2021, city became overwhelmed COVID-19 cases fatalities, as a new variant, B.1.617.2 (Delta), replaced B.1.1.7 (Alpha). A Bayesian model explains growth advantage Delta through combination increased transmissibility reduced sensitivity to immune responses generated against earlier variants...
The rapid emergence of coronavirus disease 2019 (COVID-19) as a global pandemic affecting millions individuals globally has necessitated sensitive and high-throughput approaches for the diagnosis, surveillance, determining genetic epidemiology SARS-CoV-2. In present study, we used COVIDSeq protocol, which involves multiplex-PCR, barcoding, sequencing samples detection deciphering We approach on 752 clinical in duplicates, amounting to total 1536 could be sequenced single S4 flow cell NovaSeq...
Rapid detection of DNA/RNA pathogenic sequences or variants through point-of-care diagnostics is valuable for accelerated clinical prognosis, as witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR sequencing are tough to implement with limited resources, necessitating development accurate and robust alternative strategies. Here, we report FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that utilizes a direct Cas9 based enzymatic readout detecting...
With the advent of next-generation sequencing, large-scale initiatives for mining whole genomes and exomes have been employed to better understand global or population-level genetic architecture. India encompasses more than 17% world population with extensive diversity, but is under-represented in sequencing datasets. This gave us impetus perform analyze genome 1029 healthy Indian individuals under pilot phase 'IndiGen' program. We generated a compendium 55,898,122 single allelic variants...
Abstract Delhi, the national capital of India, has experienced multiple SARS-CoV-2 outbreaks in 2020 and reached a population seropositivity over 50% by 2021. During April 2021, city became overwhelmed COVID-19 cases fatalities, as new variant B.1.617.2 (Delta) replaced B.1.1.7 (Alpha). A Bayesian model explains growth advantage Delta through combination increased transmissibility partial reduction immunity elicited prior infection (median estimates; ×1.5-fold, 20% reduction). Seropositivity...
Reinfection of SARS-CoV-2 is an apparently rare entity and only a few cases have been reported from across the world with genetic characterization virus, differentiating reinfection persistent virus shedding. These cases, therefore, provide unique insights into long term protective immunity to SARS-CoV-2. The earlier reports suggest that patients were symptomatic in either one or both episodes infection. Here we report case asymptomatic two healthcare workers India identified routine...
Long non-coding RNAs (lncRNAs) are emerging as key regulators of endothelial cell function. Here, we investigated the role a novel vascular endothelial-associated lncRNA (VEAL2) in regulating permeability. Precise editing veal2 loci zebrafish (veal2gib005Δ8/+ ) induced cranial hemorrhage. In vitro and vivo studies revealed that competes with diacylglycerol for interaction protein kinase C beta-b (Prkcbb) regulates its activity. Using PRKCB2 bait, identified functional ortholog humans from...
Abstract During the course of COVID-19 pandemic, large-scale genome sequencing SARS-CoV-2 has been useful in tracking its spread and identifying variants concern (VOC). Viral host factors could contribute to variability within a that can be captured next-generation reads as intra-host single nucleotide variations (iSNVs). Analysing 1347 samples collected till June 2020, we recorded 16 410 iSNV sites throughout genome. We found ∼42% reported SNVs by 30 September 2020 consensus sequences...
Introduction Genetic variants contribute to differential responses non-insulin antidiabetic drugs (NIADs), and consequently variable plasma glucose control. Optimal control of is paramount minimizing type 2 diabetes-related long-term complications. India’s distinct genetic architecture its exploding burden diabetes warrants a population-specific survey NIAD-associated pharmacogenetic (PGx) variants. The recent availability large-scale whole genomes from the Indian population provides unique...
Abstract Emerging reports of SARS‐CoV‐2 breakthrough infections entail methodical genomic surveillance for determining the efficacy vaccines. This study elaborates analysis isolates from following vaccination with AZD1222/Covishield and BBV152/Covaxin. Variants concern B.1.617.2 B.1.1.7 responsible cases surge in April–May 2021 Delhi, were predominant lineages among infections.
Ex vivo cellular system that accurately replicates sickle cell disease and β-thalassemia characteristics is a highly sought-after goal in the field of erythroid biology. In this study, we present generation progenitor lines with mutation using CRISPR/Cas9. The models exhibit similar differentiation profiles, globin expression proteome dynamics as patient-derived hematopoietic stem/progenitor cells. Additionally, these recapitulate pathological conditions associated both diseases. Hydroxyurea...
Ethnic differences in pharmacogenomic (PGx) variants have been well documented literature and could significantly impact variability response adverse events to therapeutics. India is a large country with diverse ethnic populations of distinct genetic architecture. India's national genome sequencing initiative (IndiGen) provides unique opportunity explore the landscape PGx using population-scale whole sequences. We analyzed IndiGen variation dataset (N = 1029 genomes) along global population...
Abstract The rapid emergence of coronavirus disease 2019 (COVID-19) as a global pandemic affecting millions individuals globally has necessitated sensitive and high-throughput approaches for the diagnosis, surveillance determining genetic epidemiology SARS-CoV-2. In present study, we used COVIDSeq protocol, which involves multiplex-PCR, barcoding sequencing samples detection deciphering We approach on 752 clinical in duplicates, amounting to total 1536 could be sequenced single S4 flow cell...
Abstract Many antibody and immune escape variants in SARS-CoV-2 are now documented literature. The availability of genome sequences enabled us to investigate the occurrence genetic epidemiology globally. Our analysis suggests that a number associated with have emerged global populations.
Coronavirus disease 2019 (COVID-19) rapidly spread from a city in China to almost every country the world, affecting millions of individuals. The rapid increase COVID-19 cases state Kerala India has necessitated understanding SARS-CoV-2 genetic epidemiology. We sequenced 200 samples patients using COVIDSeq protocol amplicon-based sequencing. analysis identified 166 high-quality single-nucleotide variants encompassing four novel and 89 new Indian isolated SARS-CoV-2. Phylogenetic haplotype...
Sequencing of SARS-CoV-2 genomes is crucial for understanding the genetic epidemiology COVID-19 pandemic. It also critical evolution virus and rapid development diagnostic tools. The present protocol a modification Illumina COVIDSeq test. We describe an amplicon-based next-generation sequencing approach with short turnaround time, adapted bench-top sequencers like MiSeq, iSeq, MiniSeq. For complete details on use execution this protocol, please refer to Bhoyar et al. (2021).
ABSTRACT Rapid detection of pathogenic sequences or variants in DNA and RNA through a point-of-care diagnostic approach is valuable for accelerated clinical prognosis as has been witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR sequencing are difficult to implement settings with limited resources necessitating development accurate alternative testing strategies that perform robustly. Here, we present FnCas9 Editor Linked Uniform Detection Assay (FELUDA)...
Hyper-IgD syndrome (HIDS, OMIM #260920) is a rare autosomal recessive autoinflammatory disorder caused by pathogenic variants in the mevalonate kinase (MVK) gene. HIDS has an incidence of 1:50,000 to 1:5,000, and thought be prevalent mainly northern Europe. Here, we report case series from India, which includes ten patients six families who presented with wide spectrum clinical features such as recurrent fever, oral ulcers, rash, arthritis, diarrhea, hepatosplenomegaly, high immunoglobulin...