A5101939013- Congenital gastrointestinal and neural anomalies
- Intestinal Malrotation and Obstruction Disorders
- Genomics and Phylogenetic Studies
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Congenital Anomalies and Fetal Surgery
- Biomedical Text Mining and Ontologies
- Advanced biosensing and bioanalysis techniques
- Digestive system and related health
- RNA and protein synthesis mechanisms
- Bacterial Identification and Susceptibility Testing
- Water Quality Monitoring and Analysis
- Herpesvirus Infections and Treatments
- Reproductive Biology and Fertility
- Renal and related cancers
- Circular RNAs in diseases
- Zebrafish Biomedical Research Applications
- Tuberculosis Research and Epidemiology
- CRISPR and Genetic Engineering
- Signaling Pathways in Disease
- Mycobacterium research and diagnosis
- Genetic Associations and Epidemiology
- Ovarian function and disorders
- Virology and Viral Diseases
- Animal Genetics and Reproduction
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2024-2025
Erasmus MC
2017-2021
Institute of Genomics and Integrative Biology
2010-2015
Council of Scientific and Industrial Research
2010
Hirschsprung disease (HSCR), which is congenital obstruction of the bowel, results from a failure enteric nervous system (ENS) progenitors to migrate, proliferate, differentiate, or survive within distal intestine. Previous studies that have searched for genes underlying HSCR focused on ENS-related pathways and not fitting current knowledge thus often been ignored. We identify validate novel using whole exome sequencing (WES), burden tests, in silico prediction, unbiased vivo analyses...
The tubercle complex consists of closely related mycobacterium species which appear to be variants a single species. Comparative genome analysis different strains could provide useful clues and insights into the genetic diversity We integrated assemblies 96 from Mycobacterium tuberculosis (MTBC), included 8 Indian clinical isolates sequenced assembled in this study, understand its pangenome architecture. predicted genes for all clustered their respective CDSs homologous gene clusters (HGCs)...
Abstract: Introduction Significant rise in resistance to tigecycline carbapenem resistant K. pneumoniae (CRKP) is now a common concern developing countries. Seeing these issues, we aimed determine the genetic prevalence of tetracycline gene along with transferability pattern pneumoniae. Methodology All isolates were isolated from pus and sputum samples identified by MALDI-TOF MS. MIC done broth micro dilution method Genetic characterization Carbapenemases (blaNDM, blaOXA-48 blaKPC) (tetA,...
Zebrafish (Danio rerio) is a popular vertebrate model organism largely deployed using outbred laboratory animals. The nonisogenic nature of the zebrafish as system offers opportunity to understand natural variations and their effect in modulating phenotype. In an effort better characterize range variation this complement reference genome project, whole sequence wild at 39-fold coverage was determined. Comparative analysis with revealed approximately 5.2 million single nucleotide over 1.6...
MicroRNAs are a well-studied class of non-coding RNA and known to regulate developmental processes in eukaryotes. Their role key biological such as vasculature development has attracted interest. However, comprehensive understanding molecular regulation angiogenesis vascular integrity during remains less explored. Here we identified miRNAs involved the maintenance zebrafish embryos using reverse genetics approach. Using combination bioinformatics predictions literature based evidences mined...
<h3>Background & Aims</h3> Hirschsprung disease (HSCR) is an inherited congenital disorder characterized by absence of enteric ganglia in the distal part gut. Variants ret proto-oncogene <i>(RET</i>) have been associated with up to 50% familial and 35% sporadic cases. We searched for variants that affect risk a large, multigenerational family history HSCR linkage region previously (4q31.3–q32.3) exome wide. <h3>Methods</h3> performed sequencing analyses Netherlands 5 members diagnosed 2...
Whole genome sequencing of personal genomes has revealed a large repertoire genomic variations and provided rich template for identification common rare variants in addition to understanding the genetic basis diseases. The widespread application clinical settings predictive preventive medicine been limited due lack comprehensive computational analysis pipelines. We have used next-generation technology sequence whole self-declared healthy male Indian origin. generated around 28X reference...
A large repertoire of gene-centric data has been generated in the field zebrafish biology. Although bulk these are available public domain, most them not readily accessible or nonstandard formats. One major challenge is to unify and integrate widely scattered sources. We tested hypothesis that active community participation could be a viable option address this challenge. present here our approach create standards for assimilation sharing information system open database intercommunication....
The advancements in genomics technologies and the amenability to large-scale computational analysis have contributed immensely understanding of zebrafish genome, its organization, functional correlates. Translating information into biological meaning would require integration data tools. FishMap is a community resource for genomic datasets on created with vision provide relevant readily available researchers. present update has kept up availability latest genome assembly (Zv8). In this...
Fast, specific identification and surveillance of pathogens is the cornerstone any outbreak response system, especially in case emerging infectious diseases viral epidemics. This process generally tedious time-consuming thus making it ineffective traditional settings. The added complexity these situations non-availability pure isolates as they are present mixed genomes or hologenomes. Next-generation sequencing approaches offer an attractive solution this scenario provides adequate depth at...
Patients with Hirschsprung disease (HSCR) do not always receive a genetic diagnosis after routine screening in clinical practice. One of the reasons for this could be that causal mutation is present cell types are usually tested-whole blood, dermal fibroblasts or saliva-but only affected tissue. Such mutations called somatic, and can occur given at any stage development conception. They will then all subsequent daughter cells. Here, we investigated presence somatic HSCR patients. For this,...