A5076803506- Blood Coagulation and Thrombosis Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Telomeres, Telomerase, and Senescence
- Atherosclerosis and Cardiovascular Diseases
- Renal Diseases and Glomerulopathies
- Antiplatelet Therapy and Cardiovascular Diseases
- Inflammasome and immune disorders
- Genomics and Phylogenetic Studies
- Chronic Kidney Disease and Diabetes
- Cardiovascular Health and Disease Prevention
- Blood Pressure and Hypertension Studies
- Pain Mechanisms and Treatments
- Renal and related cancers
- Mitochondrial Function and Pathology
- Adipokines, Inflammation, and Metabolic Diseases
- MicroRNA in disease regulation
- Protein Hydrolysis and Bioactive Peptides
- Epigenetics and DNA Methylation
- Metabolism and Genetic Disorders
- Cerebrovascular and Carotid Artery Diseases
- Biomedical Text Mining and Ontologies
- Medical and Biological Ozone Research
- Diabetes Treatment and Management
- Apelin-related biomedical research
Leipzig University
2020-2024
University Hospital Leipzig
2020-2024
Shoolini University
2024
Lovely Professional University
2010-2022
Otto-von-Guericke University Magdeburg
2020
Czech Academy of Sciences, Institute of Experimental Botany
2015
Institute of Genomics and Integrative Biology
2012-2014
Jaypee Institute of Information Technology
2013
Merck (India)
2010
Merck & Co., Inc., Rahway, NJ, USA (United States)
2006
Diabetes mellitus is a metabolic disease largely due to lifestyle and nutritional imbalance, resulting in insulin resistance, hyperglycemia vascular complications. Diabetic kidney (DKD) major cause of end-stage renal failure contributing morbidity mortality worldwide. Therapeutic options prevent or reverse DKD progression are limited. Endothelial glomerular filtration barrier (GFB) dysfunction sterile inflammation associated with DKD. Neutrophil extracellular traps (NETs), originally...
Abstract A major obstacle in diabetes is the metabolic or hyperglycemic memory, which lacks specific therapies. Here we show that glucose-mediated changes gene expression largely persist diabetic kidney disease (DKD) despite reversing hyperglycemia. The senescence-associated cyclin-dependent kinase inhibitor p21 ( Cdkn1a ) was top hit among genes persistently induced by hyperglycemia and associated with induction of p53-p21 pathway. Persistent confirmed various animal models, human samples...
Cognitive impairment is common in extracerebral diseases such as chronic kidney disease (CKD). Kidney transplantation reverses cognitive impairment, indicating that driven by CKD therapeutically amendable. However, we lack mechanistic insights allowing development of targeted therapies. Using a combination mouse models (including mice with neuron-specific IL-1R1 deficiency), single cell analyses (single nuclei RNA sequencing and thallium autometallography), human samples vitro experiments...
The Baddi-Barotiwala-Nalagarh (BBN) region of Indian Himalayas is one the most important pharmaceutical industrial clusters in Asia. This study investigated distribution, and ecological human health risks four frequently used pharmaceuticals [ciprofloxacin (CIP), norfloxacin (NOR), cetirizine (CTZ) citalopram oxalate (ECP)] when co-occurring with metal ions Sirsa river water BBN region. concentration range selected was between 'not detected' to 50 μgL
While thrombin is the key protease in thrombus formation, other coagulation proteases, such as fXa (factor Xa) or aPC (activated protein C), independently modulate intracellular signaling via partially distinct receptors.
DNA-binding protein-A (DbpA; gene: Ybx3) belongs to the cold shock protein family with known functions in cell cycling, transcription, translation, and tight junction communication. In chronic nephritis, DbpA is upregulated. However, its activities acute injury models, such as kidney ischemia/reperfusion (IRI), are unclear. To study this, mice harboring Ybx3+/+, Ybx3+/- or Ybx3-/- genotype were characterized over 24 months following experimental IRI. Mitochondrial function, number integrity...
Significance Statement Signaling to integrins is complex and depends on ligands their binding sites. Signaling-competent integrin that protect podocyte function remain unknown. This study demonstrates the coagulation protease-activated protein C (aPC) binds via its RGD sequence integrin- β 3 . Disruption of aPC–integrin- interaction results in excess RhoA activation dysfunction. These findings identify RGD-mediated as a rheostat signaling, which disrupted diabetic nephropathy....
Diabetes mellitus is hallmarked by accelerated atherosclerosis, a major cause of mortality among patients with diabetes. Efficient therapies for diabetes-associated atherosclerosis are absent. Accelerated in diabetic associated reduced endothelial thrombomodulin (TM) expression and impaired activated protein C (aPC) generation. Here, we directly compared the effects high glucose oxidized LDL, revealing that induced more pronounced responses regard to maladaptive unfolded response (UPR),...
A direct regulation of adaptive immunity by the coagulation protease activated protein C (aPC) has recently been established. Preincubation T cells with aPC for 1 hour before transplantation increases FOXP3+ regulatory (Tregs) and reduces acute graft-versus-host disease (aGVHD) in mice, but underlying mechanism remains unknown. Because cellular metabolism modulates epigenetic gene plasticity cells, we hypothesized that promotes expression altering T-cell metabolism. To this end,...
Glomerular-tubular crosstalk within the kidney has been proposed, but paracrine signals enabling this remain largely unknown. The cold-shock protein Y-box binding 1 (YBX1) is known to regulate inflammation and diseases its role in podocytes remains undetermined. Therefore, we analyzed mice with podocyte specific Ybx1 deletion (Ybx1ΔPod). Albuminuria was increased unchallenged Ybx1ΔPod mice, which surprisingly associated reduced glomerular, enhanced tubular damage. Tubular toll-like receptor...
Abstract MicroRNAs (miRNAs) have crucial functions in many cellular processes, such as differentiation, proliferation and apoptosis; aberrant expression of miRNAs has been linked to human diseases, including cancer. Tools that allow specific efficient knockdown would be immense importance for exploring miRNA function. Zebrafish serves an excellent vertebrate model system understand the involved a variety biological processes. We designed employed strategy based on locked nucleic acid enzymes...
Pregabalin and Methylcobalamine are both capable reliving the symptoms of peripheral neuropathy but their effectiveness when used in a fixed dose combination still remains subject evaluation, especially orthopaedic setting. This post marketing surveillance study evaluates efficacy tolerability Methylcobalamin 750 μg 75 mg management same. The was prescribed for 4 weeks to selected patients at 300 clinics all over India involving 1327 patients. Pain assessed on 10 point Numerical Intensity...
A large repertoire of gene-centric data has been generated in the field zebrafish biology. Although bulk these are available public domain, most them not readily accessible or nonstandard formats. One major challenge is to unify and integrate widely scattered sources. We tested hypothesis that active community participation could be a viable option address this challenge. present here our approach create standards for assimilation sharing information system open database intercommunication....
Telomerase-reverse transcriptase (TERT) plays an essential catalytic role in maintaining telomeres. However, animal systems telomerase additional non-telomeric functional roles. We previously screened Arabidopsis cDNA library for proteins that interact with the C-terminal extension (CTE) TERT domain and identified a nuclear-localized protein contains RNA recognition motif (RRM). This RRM-protein forms homodimers both plants yeast. Mutation of gene encoding had no detectable effect on plant...
Diabetes mellitus, which is largely driven by nutritional and behavioral factors, characterized accelerated atherosclerosis with impaired plaque stability. Atherosclerosis associated complications are the major cause of mortality in diabetic patients. Efficient therapeutic concepts for diabetes-associated lacking. among patients reduced endothelial thrombomodulin (TM) expression activated protein C (aPC) generation. Here, we demonstrate that atherosclerotic stability hyperglycemic mice...
Diabetic kidney disease (DKD) is an emerging pandemic, paralleling the worldwide increase in obesity and diabetes mellitus. DKD now most frequent cause of end-stage renal associated with excessive risk cardiovascular morbidity mortality. a consequence systemic endothelial dysfunction. The endothelial-dependent cytoprotective coagulation protease activated protein C (aPC) ameliorates glomerular damage DKD, part by reducing mitochondrial ROS generation cells. Whether aPC reduces tubular...
DNA-binding protein A (DbpA) belongs to the Y-box family of cold shock domain proteins that exert transcriptional and translational activities in cell via their ability bind regulate mRNA. To investigate role DbpA kidney disease, we utilized murine unilateral ureter obstruction (UUO) model, which recapitulates many features obstructive nephropathy seen humans. We observed expression is induced within renal interstitium following disease induction. Compared with wild-type animals, obstructed...