A5082046654- Atherosclerosis and Cardiovascular Diseases
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Congenital heart defects research
- Kruppel-like factors research
- Congenital Heart Disease Studies
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Inflammasome and immune disorders
- Cell Adhesion Molecules Research
- Immune responses and vaccinations
- Neurogenesis and neuroplasticity mechanisms
- Ion Transport and Channel Regulation
- Platelet Disorders and Treatments
- Barrier Structure and Function Studies
- Polysaccharides and Plant Cell Walls
- Microbial Metabolic Engineering and Bioproduction
- Proteins in Food Systems
- Tryptophan and brain disorders
- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- NF-κB Signaling Pathways
- Sodium Intake and Health
- Cardiovascular Health and Disease Prevention
Helmholtz Zentrum München
2020-2024
Leipzig University
2023
University of Münster
2017-2022
University of Freiburg
2019-2020
University Hospital Leipzig
2020
St Petersburg University
2014
Obesity promotes diverse pathologies, including atherosclerosis and dementia, which frequently involve vascular defects endothelial cell (EC) dysfunction. Each organ has distinct EC subtypes, but whether ECs are differentially affected by obesity is unknown. Here we use single-cell RNA sequencing to analyze transcriptomes of ~375,000 from seven organs in male mice at progressive stages identify organ-specific vulnerabilities. We find that deregulates gene expression networks, lipid handling,...
Cognitive impairment is common in extracerebral diseases such as chronic kidney disease (CKD). Kidney transplantation reverses cognitive impairment, indicating that driven by CKD therapeutically amendable. However, we lack mechanistic insights allowing development of targeted therapies. Using a combination mouse models (including mice with neuron-specific IL-1R1 deficiency), single cell analyses (single nuclei RNA sequencing and thallium autometallography), human samples vitro experiments...
Genetic factors undoubtedly affect the development of congenital heart disease (CHD) but still remain ill defined. We sought to identify genetic risk associated with CHD and accomplish a functional analysis SNP-carrying genes. performed genome-wide association study (GWAS) 4034 White patients 8486 healthy controls. One SNP on chromosome 5q22.2 reached significance across all phenotypes was also indicative for septal defects. region 20p12.1 pointing MACROD2 locus identified 4 highly...
Oscillatory shear stress (OSS) is an atheroprone haemodynamic force that occurs in areas of vessel irregularities and implicated the pathogenesis atherosclerosis. Changes signalling transcriptional programme response to OSS have been vigorously studied; however, underlying changes chromatin landscape controlling transcription remain be elucidated. Here, we investigated regulatory element (RE) endothelial cells under conditions vitro model.Analyses H3K27ac immunoprecipitation-Seq enrichment...
Since the generation of cell-type specific knockout models, importance inter-cellular communication between neural, vascular, and microglial cells during neural development has been increasingly appreciated. However, extent these major cell populations remains to be systematically mapped. Here, we describe EMBRACE (embryonic brain extraction using FACS), a method simultaneously isolate mural, endothelial, more than 94% purity in ∼4 h. Utilizing isolate, transcriptionally analyze, build...
Actin-binding proteins are essential for linear and branched actin filament dynamics that control shape change, cell migration, junction remodeling in vascular endothelium (endothelial cells [ECs]). The epithelial protein lost neoplasm (EPLIN) is an actin-binding protein, expressed as EPLIN-α EPLIN-β by alternative promoters; however, the isoform-specific functions not yet understood. Aortic compared to cava vein ECs shear stress-exposed cultured express increased levels stabilize stress...
Abstract Endothelial cells (ECs) lining blood vessels are exposed to mechanical forces, such as shear stress. These forces control many aspects of EC biology, including vascular tone, cell migration and proliferation. Despite a good understanding the genes responding stress, our insight into transcriptional regulation these is much more limited. Here, we set out study alterations in chromatin landscape human umbilical vein endothelial (HUVEC) laminar To do so, performed ChIP-Seq for H3K27...
Methylotrophic yeast Pichia pastoris has proved to be especially useful for production of various heterologous proteins. In biotechnology it is very important maintain the balance between high levels gene expression and cell viability. Decisive understanding regulation mechanisms essential reaching this goal. study, we investigated effect different nitrogen sources phosphate concentration in media on methanol utilization. It was shown that main genes, which are involved utilization ( MUT...
ABSTRACT Background Organ transplantation reverses cognitive impairment in chronic kidney disease (CKD), indicating that driven by CKD is therapeutically amendable. We recently demonstrated impaired cognition linked to interleukin-1β (IL-1β) release from microglia and IL-1 receptor type 1 signalling neuronal cells, thereby identifying a pathway can be exploited therapeutically. However, the mechanism of IL-1β maturation remains unknown. hypothesized cells require caspase-1 for CKD-driven...
<title>Abstract</title> Cognitive impairment is common in peripheral diseases such as chronic kidney disease (CKD). Kidney transplantation reverses cognitive impairment, indicating that driven by CKD therapeutically amendable. Yet, we lack mechanistic insights allowing targeted therapies. Using a combination of mouse models (including mice with neuron-specific IL-1R1 deficiency), single cell analyses (single nuclei RNA sequencing and thallium automethallography), human samples <italic>in...
Pericytes (PCs) are essential components of the blood brain barrier. Brain PCs critical for dynamically regulating flow, maintaining vascular integrity and their dysregulation is associated with a myriad disorders such as Alzheimer's disease. To understand physiological molecular functions, studies have increasingly focused on primary PC isolation culture. Multiple methods culture been developed over years, however, it still unclear how compare to in vivo counterparts. address this question,...
Abstract Rationale Genetic factors undoubtedly contribute to the development of congenital heart disease (CHD), but still remain mostly ill-defined. Objective Identification genetic risk associated with CHD and functional analysis SNP-carrying genes. Methods Results association study 1,440 Caucasian patients from German Heart Center Munich collected March 2009 June 2016, 2,594 previous studies provided by Newcastle University 8,486 controls underwent meta-analysis detect single nucleotide...
<title>Abstract</title> While cognitive impairment is common in peripheral diseases such as chronic kidney disease (CKD), mechanistic insights and effective therapies are lacking<sup>1,2</sup>. Organ substitution reverses impairment, indicating that driven by therapeutically amendable<sup>3,4</sup>. Yet, we lack pharmacological approaches. Here, show despite inflammasome activation the brain protection full body NLRP3-inflammasome deficient mice, microglial caspase-1 deficiency does not...
Actin-binding proteins are essential for linear and branched actin filament dynamics that control shape change, cell migration junction remodeling in vascular endothelium (ECs). The epithelial protein lost neoplasm (EPLIN) is an actin-binding protein, expressed as EPLIN-α EPLIN-β by alternative promoters, however, the isoform-specific functions not yet understood. Aortic compared to cava vein ECs shear stress-exposed cultured EC express increased levels stabilizes stress fibers. In contrast,...
Objectives: In the recent years, single cell (SC) and nuclei (SN) RNA sequencing (SC-RNAseq, SN-RNAseq) became state-of-the art to analyze complexity of tissue transcriptomes. We sought uncover cellular composition in different localizations adult human heart compare nuclear transcription profiles by SC-RNAseq SN-RNAseq, respectively.
Abstract Endothelial cells (EC) lining blood vessels are exposed to mechanical forces, such as shear stress exerted by the flowing blood. These forces control many aspects of EC biology, including vascular tone, cell migration and proliferation in addition size shape. Despite a good understanding genes signaling pathways responding stress, our insights into transcriptional regulation these responses is much more limited. In particular, we do not know different sets regulatory elements...