A5071051098- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Mast cells and histamine
- Liver physiology and pathology
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- Bioinformatics and Genomic Networks
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Pancreatic function and diabetes
- Congenital gastrointestinal and neural anomalies
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Congenital heart defects research
- Renal and related cancers
- Ferroptosis and cancer prognosis
- Mesenchymal stem cell research
- Pancreatic and Hepatic Oncology Research
- Acute Myeloid Leukemia Research
- NF-κB Signaling Pathways
Max Planck Institute of Immunobiology and Epigenetics
2019-2023
Friedrich Miescher Institute
2023
University of Freiburg
2019
Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development compare them with the in vivo embryo. We developed high-throughput handling imaging pipeline to spatially monitor symmetry breaking report an early spatial variability pluripotency determining binary response Wnt activation. Although...
Abstract Bone marrow haematopoietic stem cells (HSCs) are vital for lifelong maintenance of healthy haematopoiesis. In inbred mice housed in gnotobiotic facilities, the top hierarchy is occupied by dormant HSCs, which reversibly exit quiescence during stress. Whether HSC dormancy exists humans remains debatable. Here, using single-cell RNA sequencing, we show a continuous landscape highly purified human bone HSCs displaying varying degrees dormancy. We identify orphan receptor GPRC5C,...
Abstract Immune cell locomotion is associated with amoeboid migration, a flexible mode of movement, which depends on rapid cycles actin polymerization and actomyosin contraction 1 . Many immune cells do not necessarily require integrins, the major family adhesion receptors in mammals, to move productively through three-dimensional tissue spaces 2,3 Instead, they can use alternative strategies transmit their actin-driven forces substrate, explaining migratory adaptation changing external...
Since the generation of cell-type specific knockout models, importance inter-cellular communication between neural, vascular, and microglial cells during neural development has been increasingly appreciated. However, extent these major cell populations remains to be systematically mapped. Here, we describe EMBRACE (embryonic brain extraction using FACS), a method simultaneously isolate mural, endothelial, more than 94% purity in ∼4 h. Utilizing isolate, transcriptionally analyze, build...
Hepatocellular carcinoma (HCC) is the sixth most common cancer and third cause of cancer-related death, with tumour associated liver endothelial cells being thought to be major drivers in HCC progression. This study aims compare gene expression profiles from non-tumour tissue, identify perturbed biologic functions, co-expression modules, potentially drugable hub genes that could give rise novel therapeutic targets strategies. Gene Set Variation Analysis (GSVA) showed cell growth-related...
ABSTRACT The human liver is an essential multifunctional organ, and diseases are rising with limited treatment options. However, the cellular complexity heterogeneity of remain poorly understood. Here, we performed single-cell RNA-sequencing ~5,000 cells from normal tissue 6 donors to construct first cell atlas. Our analysis revealed previously unknown sub-types among endothelial cells, Kupffer hepatocytes transcriptome-wide zonation these populations. We show that EPCAM + population highly...
SUMMARY Many leukocytes use fast and flexible amoeboid migration strategies to move autonomously throughout tissues. Here, we show that the movement of mast cells (MCs), with important roles during allergies anaphylaxis, fundamentally differs from this rapid adhesion-free leukocyte migration. We identify a crucial role for integrin-dependent adhesion in controlling slow MC movement, which shapes positioning network-like tissue distribution long-lived immune cell type. In contrast other...
Abstract This is a protocol describing how to perform scRNA-seq of cryopreserved human liver cells using the mCEL-Seq2 as described in following paper.Title: A Human Liver Cell Atlas reveals Heterogeneity and Epithelial ProgenitorsAuthors: Nadim Aizarani, Antonio Saviano, Sagar, Laurent Mailly, Sarah Durand, Patrick Pessaux, Thomas F. Baumert, Dominic GrünJournal: Nature