A5000127861- Cellular Mechanics and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Digestive system and related health
- Photoacoustic and Ultrasonic Imaging
- Congenital heart defects research
- Hippo pathway signaling and YAP/TAZ
- Liver physiology and pathology
- Congenital gastrointestinal and neural anomalies
Friedrich Miescher Institute
2023-2025
University of Basel
2023-2025
Birkbeck, University of London
2021-2022
Institute of Structural and Molecular Biology
2021-2022
Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development compare them with the in vivo embryo. We developed high-throughput handling imaging pipeline to spatially monitor symmetry breaking report an early spatial variability pluripotency determining binary response Wnt activation. Although...
Abstract Multicellular systems grow over the course of weeks from single cells to tissues or even full organisms, making live imaging challenging. To bridge spatiotemporal scales, we present an open-top dual-view and dual-illumination light-sheet microscope dedicated large specimens at single-cell resolution. The configuration objectives together with a customizable multiwell mounting system combines dual view high-throughput multiposition imaging. We use this image wide variety samples...
Abstract Tissue regeneration relies on the ability of cells to undergo de novo patterning. While tissue patterning has been viewed as transition from initially identical un-patterned an arrangement different cell types, recent evidence suggests that initial heterogeneities between modulate tissue-scale pattern formation. Yet, how such arise and, thereafter, regulate type emergence in a population is poorly understood. Using vivo and vitro mouse regenerative systems, we identify critical...
Abstract Protein phosphorylation is a major regulatory mechanism of cellular signalling. The c-JUN proto-oncoprotein phosphorylated at four residues within its transactivation domain (TAD) by the JNK family kinases, but functional significance multisite has remained elusive. Here we show that exhibits defined temporal kinetics, with serine63 and serine73 being more rapidly than threonine91 threonine93. We identify positioning sites relative to kinase docking motif, their primary sequence, as...
Summary The human intestinal epithelium is a tissue with rapid turnover. Its complex regenerative process and differentiation trajectories have been challenging to study due its inaccessibility lack of temporal sampling. To this end, we developed workflow culture adult stem cell-derived organoids from single cells maturation. Extensive characterization our model system indicated transient response, followed by into all mature cell lineages. This switch accompanied transition between two...
Abstract Multicellular systems grow over the course of weeks from single cells to tissues or even full organisms composed several thousands cells, making live imaging such samples challenging. To bridge these wide spatiotemporal scales, we present an open top dual view and illumination light sheet microscope dedicated for large specimens at cell resolution. The novel configuration objectives together with a flexible customizable multi-well mounting system combines high throughput...
Abstract Protein phosphorylation is a major regulatory mechanism of cellular signalling. The c-Jun proto-oncoprotein phosphorylated at four residues within its transactivation domain (TAD) by the JNK family kinases, but functional significance multisite has remained elusive. Here we show that exhibits defined temporal kinetics, with serine63 and serine73 being more rapidly than threonine91 threonine93. We identified positioning sites relative to kinase docking motif, their primary sequence,...