A5026526719- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Liver physiology and pathology
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- Organ Transplantation Techniques and Outcomes
- Birth, Development, and Health
- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Liver Disease and Transplantation
- Cancer-related gene regulation
- Microbial Metabolic Engineering and Bioproduction
- Phagocytosis and Immune Regulation
- ATP Synthase and ATPases Research
- Cancer Immunotherapy and Biomarkers
- Metabolism and Genetic Disorders
- Barrier Structure and Function Studies
- Renal Transplantation Outcomes and Treatments
- Cardiac Fibrosis and Remodeling
- Cardiovascular Health and Disease Prevention
- Fermentation and Sensory Analysis
- Cardiovascular Disease and Adiposity
Leipzig University
2023
Helmholtz Zentrum München
2022-2023
Universidad Nacional Autónoma de México
2017-2023
Centre International de Recherche sur le Cancer
2019
Consejo Superior de Investigaciones Científicas
2004
Instituto de Agroquímica y Tecnología de Alimentos
2004
Delft University of Technology
2004
Abstract Mitochondrial dysfunction plays critical roles in cancer development and related therapeutic response; however, exact molecular mechanisms remain unclear. Recently, alongside the discovery of mitochondrial-specific DNA methyltransferases, global site-specific methylation mitochondrial genome has been described. Investigation any functional consequences however remains unclear debated due to insufficient evidence quantitative degree frequency (mtDNA) methylation. This study uses WGBS...
Obesity promotes diverse pathologies, including atherosclerosis and dementia, which frequently involve vascular defects endothelial cell (EC) dysfunction. Each organ has distinct EC subtypes, but whether ECs are differentially affected by obesity is unknown. Here we use single-cell RNA sequencing to analyze transcriptomes of ~375,000 from seven organs in male mice at progressive stages identify organ-specific vulnerabilities. We find that deregulates gene expression networks, lipid handling,...
Abstract A basic question linked to differential patterns of gene expression is how cells reach different fates despite using the same DNA template. Since 5-hydroxymethylcytosine (5hmC) emerged as an intermediate metabolite in active demethylation, there have been increasing efforts elucidate its function a stable modification genome, including role establishing such tissue-specific expression. Recently we described TET1-mediated enrichment 5hmC on promoter region master regulator hepatocyte...
Abstract Mammalian cytosine DNA methylation (5mC) is associated with the integrity of genome and transcriptional status nuclear DNA. Due to technical limitations, it has been less clear if mitochondrial (mtDNA) methylated whether 5mC a regulatory role in this context. Here, we used bisulfite-independent single-molecule sequencing native human mouse study across different biological conditions. We first validated ability long-read nanopore detect CpG (5mCpG) non-CpG (5mCpH) context at...
Regulatory T cells are considered key players in the prevention of allograft rejection transplanted patients. Belatacept, is an effective alternative to calcineurin inhibitors that appears preserve graft survival and function, however, impact this drug homeostasis Tregs patients remains controversial. Here we analyzed phenotype, function epigenetic status Treg-specific demethylated region (TSDR) FOXP3 circulating from long-term kidney transplant under Belatacept or Cyclosporine A treatment....
Physiological effects of carbon dioxide and impact on genome-wide transcript profiles were analysed in chemostat cultures Saccharomyces cerevisiae. In anaerobic, glucose-limited grown at atmospheric pressure, cultivation under CO2-saturated conditions had only a marginal (<10%) the biomass yield. Conversely, 25% decrease yield was found aerobic, aerated with mixture 79% CO2 21% O2. This observation indicated that respiratory metabolism is more sensitive to than fermentative metabolism....
Chronic hypoxia is a major contributor to Kidney Disease (CKD) after Acute Injury (AKI). However, the temporal relation between acute insult and maladaptive renal response remains unclear. In this study, we analyzed time-course of hemodynamics, oxidative stress, inflammation, fibrosis, as well epigenetic modifications, with focus on HIF1α/VEGF signaling, in AKI CKD transition. Sham-operated, right nephrectomy (UNx), UNx plus ischemia (IR + UNx) groups rats were included studied at 1, 2, 3,...
Methylglyoxal (MG), a glycolytic by-product, is an extremely toxic compound. This fact suggests that its synthesis and degradation should be tightly controlled. However, little known about the mechanisms protect yeast cells against MG toxicity. Here, we show in Saccharomyces cerevisiae, exposure increased internal content activated expression of GLO1 GRE3, two genes involved detoxification; GPD1, gene for glycerol synthesis; TPS1 TPS2, trehalose pathway genes. response was specific as...
Recently described as the sixth base of DNA macromolecule, precise role 5-hydroxymethylcytosine (5hmC) is subject debate. Early studies indicate that it functionally distinct from cytosine methylation (5mC), and there evidence for 5hmC being a stable derivate 5mC, rather than just an intermediate demethylation. Moreover, events correlate in time space with key differentiation steps mammalian cells. Such span three embryonic germ layers multiple progenitor cell subtypes, suggesting general...
Maternal nutrition during gestation has important effects on gene expression-mediated metabolic programming in offspring. To evaluate the effect of a protein-restricted maternal diet gestation, pancreatic islets from male progeny Wistar rats were studied at postnatal days (PND) 36 (juveniles) and 90 (young adults). The expression key genes involved β-cell function DNA methylation pattern regulatory regions two such genes, Pdx1 (pancreatic duodenal homeobox 1) MafA (musculoaponeurotic...
Background: Mitochondrion is an important metabolic and energetic organelle that regulates several cellular processes. Mitochondrial dysfunction has been related to liver diseases including hepatocellular carcinoma. As a result, the demand not properly supplied mitochondrial morphologic changes have observed, resulting in altered metabolism. We previously demonstrated chemopreventive effect of hepatoprotector IFC-305. Aim: In this work we aimed evaluate functional, metabolic, dynamic...
The pathological characteristic of cirrhosis is scarring which results in a structurally distorted and dysfunctional liver. Previously, we demonstrated that Col1a1 Pparg genes are deregulated CCl4 -induced but their normal expression levels recovered upon treatment with IFC-305, an adenosine derivative. We observed was able to modulate S-adenosylmethionine-dependent trans-methylation reactions, recently, found IFC-305 modulates HDAC3 expression. Here, investigated whether epigenetic...
Pericytes (PCs) are essential components of the blood brain barrier. Brain PCs critical for dynamically regulating flow, maintaining vascular integrity and their dysregulation is associated with a myriad disorders such as Alzheimer's disease. To understand physiological molecular functions, studies have increasingly focused on primary PC isolation culture. Multiple methods culture been developed over years, however, it still unclear how compare to in vivo counterparts. address this question,...
Abstract Cytosine DNA methylation in the CpG context (5mCpG) is associated with transcriptional status of nuclear DNA. Due to technical limitations, it has been less clear if mitochondrial (mtDNA) methylated and whether 5mCpG a regulatory role this context. The main aim work was develop validate novel tool for determining mtDNA corroborate its existence across different biological contexts. Using long-read nanopore sequencing we found low levels (with few exceptions) little variation...
Abstract How cells reach different fates despite using the same DNA template, is a basic question linked to differential patterns of gene expression. Since 5-hydroxymethylcytosine (5hmC) emerged as an intermediate metabolite in active demethylation, there have been increasing efforts elucidate its function stable modification genome, including role establishing such tissue-specific Recently we described TET1-mediated enrichment 5hmC on promoter region master regulator hepatocyte identity,...
Liver fibrosis is a global health problem, and studying its development provides important information to address treatment. Here, we characterized the effects of an adenosine compound (IFC-305) on preventing liver inflammation.We studied impact IFC-305 carbon tetrachloride-induced model in Wistar male rats at 4, 6, 8 weeks. The were by tissue histology, macrophages identification flow cytometry with CD163+/CD11b/c+ antibodies, hepatic plasmatic cytokine levels employing MILLIPLEX MAP ELISA,...