A5032206449- Celiac Disease Research and Management
- Helicobacter pylori-related gastroenterology studies
- Genetic factors in colorectal cancer
- Glioma Diagnosis and Treatment
- Galectins and Cancer Biology
- Colorectal Cancer Screening and Detection
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Treatments and Studies
- Educational theories and practices
- Ubiquitin and proteasome pathways
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Neurogenesis and neuroplasticity mechanisms
- Higher Education Teaching and Evaluation
- Tunneling and Rock Mechanics
- Neuroinflammation and Neurodegeneration Mechanisms
- Bioinformatics and Genomic Networks
- Immune cells in cancer
- Medicine and Dermatology Studies History
- Mechanical stress and fatigue analysis
- Basque language and culture studies
- Genetic diversity and population structure
- Educational Technology in Learning
University of the Basque Country
2014-2023
Centre National de la Recherche Scientifique
2021-2022
Institut de Biochimie et Génétique Cellulaires
2020-2022
BioCruces Health research Institute
2016-2021
Université de Bordeaux
2021
Abstract Mitochondrial dysfunction plays critical roles in cancer development and related therapeutic response; however, exact molecular mechanisms remain unclear. Recently, alongside the discovery of mitochondrial-specific DNA methyltransferases, global site-specific methylation mitochondrial genome has been described. Investigation any functional consequences however remains unclear debated due to insufficient evidence quantitative degree frequency (mtDNA) methylation. This study uses WGBS...
Abstract Lactate is a central metabolite in brain physiology but also contributes to tumor development. Glioblastoma (GB) the most common and malignant primary adults, recognized by angiogenic invasive growth, addition its altered metabolism. We show herein that lactate fuels GB anaplerosis replenishing tricarboxylic acid (TCA) cycle absence of glucose. dehydrogenases (LDHA LDHB), which we found spatially expressed tissues, catalyze interconversion pyruvate lactate. However, ablation both...
N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in regulation of several biological processes, including immune response, different approaches been developed order to map quantify m6A marks. However, site specific detection methylation has technically challenging, existing protocols are long tedious often involve next-generation sequencing. Here, we describe a simple RT-QPCR based approach for relative quantification...
Azterlan hone xede nagusia da Derrigorrezko Bigarren Hezkuntza etapako irakasleek STEM hezkuntza-ereduarekiko duten pertzepzioa aztertzea, irakaskuntza-ikaskuntza prozesuen ezarpenean eragina duelako. Alegia, irakasleak ereduaren ezarpenaren eragile nagusiak izanik, gauzatuko dituzten praktikak marko horren inguruan pertzepzioaren araberakoak izango dira. Helburu hori betetzeko asmoz, Gipuzkoako sare guztietako 5 ikastetxetako Natur Zientziak, Teknologia eta Matematika ezagutza-arloetako 12...
Celiac disease is a chronic immune-mediated disorder with an important genetic component. To date, there are 57 independent association signals from 39 non-HLA loci, and total of 66 candidate genes have been proposed. We aimed to scrutinize the functional implication 45 those by analyzing their expression in tissue celiac patients (at diagnosis/treatment) compared non-celiac controls. Moreover, we investigated SNP genotype effect gene performed coexpression analyses. Several showed...
Abstract The Human Leucocyte Antigen (HLA) locus and other DNA sequence variants identified in Genome-Wide Association (GWA) studies explain around 50% of the heritability celiac disease (CD). However, pathogenesis CD could be driven by layers genomic information independent from variation, such as methylation, it is possible that allele-specific methylation explains part SNP associations. Since landscape expected to different among cell types, we analyzed methylome epithelial immune...
The aim of the study is to identify additional celiac disease associated loci in major histocompatibility complex (MHC) independent from classical HLA risk alleles (HLA-DR3-DQ2) and characterize their potential functional impact pathogenesis at intestinal level.We performed a high-resolution single-nucleotide polymorphism (SNP) genotyping MHC region, comparing HLA-DR3 homozygous patients non-celiac controls carrying single copy B8-DR3-DQ2 conserved extended haplotype. Expression level novel...
Celiac disease (CD) patients present a loss of intestinal barrier function due to structural alterations in the tight junction (TJ) network, most apical unions between epithelial cells. The association TJ-related gene variants points an implication this network susceptibility. This work aims characterize functional TJ-related, disease-associated loci CD pathogenesis. We performed study 8 cohort 270 and 91 non-CD controls. expression level transcripts located associated SNP region was...
Although the genetic contribution to colorectal cancer (CRC) has been studied in various populations, studies on applicability of available information Basque population are scarce. In total, 835 CRC cases and 940 controls from were genotyped genome-wide association carried out. Mendelian Randomization analyses used discover effect modifiable risk factors microbiota CRC. 25 polygenic score models evaluated assess their performance calculation. Moreover, 492 inflammatory bowel disease whether...
The aim of this study was to construct celiac co-expression patterns at a whole genome level and identify transcription factors (TFs) that could drive the gliadin-related changes in coordination gene expression observed disease (CD). Differential modules were identified acute chronic responses gliadin using data from previous microarray duodenal biopsies. Transcription factor binding site (TFBS) Gene Ontology (GO) annotation enrichment analyses performed differentially co-expressed genes...
This study aimed to test mate choice and selection during early life stages on major histocompatibility (MH) genotype in natural families of Atlantic salmon Salmo salar spawners juveniles, using nine microsatellites reconstruct families, one microsatellite linked an MH class I gene minisatellite II gene. MH-based was only detected for the locus first year, with lower expected heterozygosity offspring actually mated pairs than predicted under random mating. The frequencies MH-linked loci...
Background: Some genetic polymorphisms (SNPs) have been proposed as predictors for different colorectal cancer (CRC) outcomes. This work aims to assess their performance in our cohort and find new SNPs associated with them. Methods: A total of 833 CRC cases were analyzed seven outcomes, including the use chemotherapy, stratified by tumor location stage. The 63 was assessed using a generalized linear model area under receiver operating characteristic curve, local detected logistic...
Abstract Lactate is a central metabolite in brain physiology, involved the astrocyte-neuron lactate shuttle, but also contributes to tumor development. Glioblastoma (GBM) most common and malignant primary adults, recognized by angiogenic invasive growth, addition its altered metabolism. By adapting their glycolytic or oxidative metabolism, GBM stem-like cells are able resist chemo- radiotherapy. We show herein that fuels anaplerosis replenishing TCA cycle absence of glucose. dehydrogenases...
The purpose of this protocol is to fractionate human intestinal tissue obtained by endoscopy into nuclear and cytoplasmic compartments for the localization analysis specific proteins or protein complexes in different states (i.e., healthy vs. disease). This method useful fractionation both fresh frozen samples; it easily accessible all laboratories not time consuming.
Glioblastomas (GBMs), grade IV malignant gliomas, are one of the deadliest types human cancer because their aggressive characteristics. Despite significant advances in genetics these tumors, how GBM cells invade healthy brain parenchyma is not well understood. Notably, it has been shown that peritumoral space via different routes; main interest this paper route along white matter tracts (WMTs). The interactions tumor with nervous cell components characterized. Herein, a method described...
Abstract Glioblastoma (GBM) is a common and devastating brain tumor, associated with low median survival, despite standard therapeutic management. Among its major features, GBMs are highly angiogenic, infiltrative, exhibit paradoxically an elevated glycolysis. Most of differentiated cells convert glucose into pyruvate that enters the Krebs cycle to maximize energy production in presence oxygen. For cancer cells, uptake catabolism increased regardless oxygen level. However, tumor cell needs...
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