A5048624077- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Biotin and Related Studies
- Peptidase Inhibition and Analysis
- Renal and related cancers
- Click Chemistry and Applications
- Autophagy in Disease and Therapy
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Genetic diversity and population structure
- Molecular Junctions and Nanostructures
- Renal cell carcinoma treatment
- 14-3-3 protein interactions
- Genetic and phenotypic traits in livestock
- Eicosanoids and Hypertension Pharmacology
- Genetic and Kidney Cyst Diseases
- Chromatin Remodeling and Cancer
- Cellular transport and secretion
- Protease and Inhibitor Mechanisms
- Cholesterol and Lipid Metabolism
- Genetics and Neurodevelopmental Disorders
- Chemical Synthesis and Analysis
- Animal Behavior and Reproduction
- Cancer, Lipids, and Metabolism
CIC bioGUNE
2020-2025
BioCruces Health research Institute
2025
University of the Basque Country
2017
Abstract The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation TurboID protein-fragment complementation to find SUMO-dependent interactors proteins interest. We develop an optimized split-TurboID version show SUMO interaction-dependent labelling proximal PML RANGAP1. are involved in transcription, DNA damage, stress response modification...
Hundreds of E3 ligases play a critical role in recognizing specific substrates for modification by ubiquitin (Ub). Separating genuine targets E3s from E3-interactors remains challenge. We present BioE3, powerful approach matching to Ub interest. Using BirA-E3 ligase fusions and bioUb, site-specific biotinylation Ub-modified particular facilitates proteomic identification. show that BioE3 identifies both known new two RING-type ligases: RNF4 (DNA damage response, PML bodies), MIB1...
Primary cilia are sensory organelles crucial for cell signaling during development and organ homeostasis. Cilia arise from centrosomes their formation function is governed by numerous factors. Through our studies on Townes-Brocks Syndrome (TBS), a rare disease linked to abnormal in human fibroblasts, we uncovered the leucine-zipper protein LUZP1 as an interactor of truncated SALL1, dominantly-acting causing disease. Using TurboID proximity labeling pulldowns, show that associates with...
Abstract Background The specificity of the ubiquitination process is mediated by E3 ligases. Discriminating genuine substrates E3s from mere interacting proteins one major challenges in field. We previously developed BioE3, a biotin-based approach that uses BirA-E3 fusions together with ubiquitin fused to low-affinity AviTag obtain site-specific and proximity-dependent biotinylation substrates. proved suitability BioE3 identify targets RING HECT-type Methods experiments were performed...
Abstract Background The specificity of the ubiquitination process is mediated by E3 ligases. Discriminating genuine substrates E3s from mere interacting proteins one major challenges in field. We previously developed BioE3, a biotin-based approach that uses BirA-E3 fusions together with ubiquitin fused to low-affinity AviTag obtain site-specific and proximity-dependent biotinylation substrates. proved suitability BioE3 identify targets RING HECT-type Methods experiments were performed...
Abstract A complex code of cellular signals is mediated by ubiquitin and ubiquitin‐like (Ub/UbL) modifications on substrate proteins. The so‐called Ubiquitin Code specifies protein fates, such as stability, subcellular localization, functional activation or suppression, interactions. Hundreds enzymes are involved in placing removing Ub/UbL thousands substrates, while the consequences mechanisms specificity still poorly defined. Challenges include rapid transient engagement interactors, low...
This study aimed to test mate choice and selection during early life stages on major histocompatibility (MH) genotype in natural families of Atlantic salmon Salmo salar spawners juveniles, using nine microsatellites reconstruct families, one microsatellite linked an MH class I gene minisatellite II gene. MH-based was only detected for the locus first year, with lower expected heterozygosity offspring actually mated pairs than predicted under random mating. The frequencies MH-linked loci...
Development is orchestrated through a complex interplay of multiple transcription factors. The comprehension this will help us to understand developmental processes. Here we analyze the relationship between two key factors: CBX4, member Polycomb Repressive Complex 1 (PRC1), and SALL1, Spalt-like family with important roles in embryogenesis limb development. Both proteins localize nuclear bodies are modified by small ubiquitin-like modifier (SUMO). Our results show that CBX4 SALL1 interact...
ABSTRACT The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation TurboID protein-fragment complementation to find SUMO-dependent interactors proteins interest. We developed an optimized split-TurboID version show SUMO interaction-dependent labelling proximal PML RANGAP1. are involved in transcription, DNA damage, stress response...
ABSTRACT The post-translational modification of proteins by ubiquitination is a highly regulated process that involves dynamic, three-step enzymatic cascade, where more than 600 E3 ligases play critical role in recognizing specific substrates for modification. Separating bona fide targets E3s from E3-interacting remains major challenge the field. In this study, we present BioE3, novel approach identifying ubiquitin-like (UbL) interest. Using BirA-E3 ligase fusion and bioUbLs, method...