A5053687498- Bone Tissue Engineering Materials
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Protein Structure and Dynamics
- Ocular Surface and Contact Lens
- Metabolomics and Mass Spectrometry Studies
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Ferroptosis and cancer prognosis
- Reproductive System and Pregnancy
- Protein Hydrolysis and Bioactive Peptides
- Carbohydrate Chemistry and Synthesis
- Polymer Surface Interaction Studies
- Salivary Gland Disorders and Functions
- Spectroscopy Techniques in Biomedical and Chemical Research
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Bone health and osteoporosis research
- Inflammatory Bowel Disease
- HIV Research and Treatment
- Spectroscopy and Chemometric Analyses
- Meat and Animal Product Quality
- Extracellular vesicles in disease
- Spectroscopy and Quantum Chemical Studies
CIC bioGUNE
2016-2025
Centro de Investigación Biomédica en Red
2013-2025
Instituto de Salud Carlos III
2012-2025
Euskadiko Parke Teknologikoa
2015-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2010-2024
Centro de Edafología y Biología Aplicada del Segura
2010
Hospital de Cruces
2009
University of Puerto Rico-Mayaguez
2006-2008
University of the Basque Country
1999-2006
Universidad de Salamanca
1999
The endometrial fluid is a noninvasive sample which contains numerous secreted proteins representative of function and reflects the state endometrium. In this study, we describe, for first time, comprehensive catalogue during secretory phase menstrual cycle. To achieve objective, three different but complementary strategies were used: First, in-solution digestion followed by reverse high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS); second, protein...
Plasma rich in growth factors (PRGF®-Endoret®) is an autologous technology that contains a set of proteins specifically addressed to wound healing and tissue regeneration. The scaffold formed by using this clot mainly composed fibrin protein, forming three-dimensional (3D) macroscopic network. This biomaterial easily obtained biotechnological means from blood can be used range situations help Although the main constituent scaffold, little known about other interacting may act as adjuvants...
Hundreds of E3 ligases play a critical role in recognizing specific substrates for modification by ubiquitin (Ub). Separating genuine targets E3s from E3-interactors remains challenge. We present BioE3, powerful approach matching to Ub interest. Using BirA-E3 ligase fusions and bioUb, site-specific biotinylation Ub-modified particular facilitates proteomic identification. show that BioE3 identifies both known new two RING-type ligases: RNF4 (DNA damage response, PML bodies), MIB1...
Abstract Background The specificity of the ubiquitination process is mediated by E3 ligases. Discriminating genuine substrates E3s from mere interacting proteins one major challenges in field. We previously developed BioE3, a biotin-based approach that uses BirA-E3 fusions together with ubiquitin fused to low-affinity AviTag obtain site-specific and proximity-dependent biotinylation substrates. proved suitability BioE3 identify targets RING HECT-type Methods experiments were performed...
We have studied the secondary structure of carboxyl-terminal domains linker histone H1 subtypes H1(0) (C-H1(0)) and H1t (C-H1t), free in solution bound to DNA, by IR spectroscopy. The domain has little aqueous but becomes extensively folded upon interaction with DNA. elements present include alpha-helix, beta-structure, turns, open loops. shows a significant dependence on salt concentration. In low (10 mm NaCl), there is residual amount random coil, 7% C-H1(0) 12% C-H1t. physiological...
Endogenous peptides are valuable targets in the analysis of biological processes. The tear film contains proteins and released by duct mucosal cells, including antimicrobial involved protection against exogenous pathogens; however, peptide content liquid remains poorly characterized. We analyzed naturally occurring isolated from human basal tears. Mass spectrometry endogenous presents a number drawbacks, size heterogeneity nonpredictable fragmentation patterns, among others. Therefore, CID,...
Titanium dental implants are commonly used due to their biocompatibility and biochemical properties; blasted acid-etched Ti is more frequently than smooth surfaces. In this study, physico-chemical characterisation revealed important differences in roughness, chemical composition hydrophilicity, but no were found cellular vitro studies (proliferation mineralization). However, the deposition of proteins onto implant surface might affect vivo osseointegration. To test that hypothesis, protein...
Olfactory dysfunction is one of the earliest features in Lewy-type alpha-synucleinopathies (LTSs) such as Parkinson's disease (PD). However, underlying molecular mechanisms associated to smell impairment are poorly understood. Applying mass spectrometry–based quantitative proteomics postmortem olfactory bulbs across limbic, early-neocortical, and neocortical LTS stages parkinsonian patients, a proteostasis impairment, was observed, identifying 268 differentially expressed proteins between...
Abstract The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation TurboID protein-fragment complementation to find SUMO-dependent interactors proteins interest. We develop an optimized split-TurboID version show SUMO interaction-dependent labelling proximal PML RANGAP1. are involved in transcription, DNA damage, stress response modification...
The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology Crohn’s disease (CD) and ulcerative colitis (UC), as well biomarker discovery. To characterize composition EVs in patients with CD UC to identify biomarkers molecular pathways associated pathogenesis activity. Methods: Serum were enriched analyzed quiescent CD, active (aCD), UC, (aUC), healthy controls (HCs) (n = 30 per group). All groups matched age sex. Disease activity was...
Abstract Background Inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), are complex conditions characterized by significant clinical, immunological, molecular, genetic, microbiological heterogeneity. Current therapeutic strategies, particularly biologics Janus kinase (JAK) inhibitors, effective for inducing clinical remission controlling inflammation. However, one-third of patients fail to respond these treatments, highlighting the need predictive...
Abstract Background The specificity of the ubiquitination process is mediated by E3 ligases. Discriminating genuine substrates E3s from mere interacting proteins one major challenges in field. We previously developed BioE3, a biotin-based approach that uses BirA-E3 fusions together with ubiquitin fused to low-affinity AviTag obtain site-specific and proximity-dependent biotinylation substrates. proved suitability BioE3 identify targets RING HECT-type Methods experiments were performed...
Is there any difference in the protein composition of endometrial fluid aspirate (EFA) obtained day embryo transfer vitro fertilization (IVF) cycles achieving and not pregnancy?Comparative analysis identified a differential expression pattern 'implantative' 'non-implantative' IVF cycles.EFA allows non-invasive characterization endometrium, may contain important information on its receptivity when performing cycles. Endometrial side implantation has usually been studied with biopsy an cycle...
The interactions of implanted biomaterials with the host organism determine success or failure an implantation. Normally, their biocompatibility is assessed using in vitro tests. Unfortunately, and vivo results are not always concordant; new, effective methods biomaterial characterisation urgently needed to predict outcome. As first layer proteins adsorbed onto surfaces might condition response, mass spectrometry analysis was performed characterise these proteins. Four distinct hybrid...
Abstract Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there an inexorable deterioration with a poor prognosis; currently are no therapies capable modifying disease progression and aortic replacement only available treatment. Our goal to study calcification by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) get new insights at molecular level that could help in understanding this In work, we analyzed consecutive slices...
Primary cilia are sensory organelles crucial for cell signaling during development and organ homeostasis. Cilia arise from centrosomes their formation function is governed by numerous factors. Through our studies on Townes-Brocks Syndrome (TBS), a rare disease linked to abnormal in human fibroblasts, we uncovered the leucine-zipper protein LUZP1 as an interactor of truncated SALL1, dominantly-acting causing disease. Using TurboID proximity labeling pulldowns, show that associates with...
The composition of endometrial fluid reflects the status endometrium; it is a good atraumatic source information on embryo implantation processes and possible pathological conditions. Although some attempts have been made to characterise its proteome, catalogue proteins remains incomplete little has done analyse natural peptides contains. Here, we present comprehensive analysis fluid. protein content samples from 11 individuals was analysed using novel timsTOF Pro mass spectrometer. We...
The stability of a lentil lectin, an all-β protein, has been perturbed by changes in pH and temperature. In the interval 5.0 → 10.0, overall secondary structure does not undergo significant changes. However, if individual components infrared amide I band are considered, attributed to variations β-sheet β-turns cross-interactions detected. combined effects temperature reveal that protein is more compact at 7.5 with lower denaturation temperatures or indicating less stable under those...