A5012180081- Ubiquitin and proteasome pathways
- Neurobiology and Insect Physiology Research
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- Cancer-related gene regulation
- Invertebrate Immune Response Mechanisms
- Peptidase Inhibition and Analysis
- Insect and Arachnid Ecology and Behavior
- Glycosylation and Glycoproteins Research
- Autophagy in Disease and Therapy
- Toxoplasma gondii Research Studies
- Genetics and Neurodevelopmental Disorders
- Insect Resistance and Genetics
- Medication Adherence and Compliance
- Pancreatic function and diabetes
- Adenosine and Purinergic Signaling
- Histone Deacetylase Inhibitors Research
- Attention Deficit Hyperactivity Disorder
- bioluminescence and chemiluminescence research
- Heat shock proteins research
- Pharmaceutical studies and practices
- Insect and Pesticide Research
- Signaling Pathways in Disease
- Alzheimer's disease research and treatments
- Biotin and Related Studies
CIC bioGUNE
2010-2025
Key scientific discoveries have resulted from genetic studies of
Abstract Animals have a determined species-specific body size that results from the combined action of hormones and signaling pathways regulating growth rate duration. In Drosophila , steroid hormone ecdysone controls developmental transitions, thereby duration period. Here we show promotes imaginal discs in mid-third instar larvae, since larvae with reduced or no synthesis are smaller than wild type due to fewer cells. We insulin-like peptides produced secreted normally upstream components...
Hundreds of E3 ligases play a critical role in recognizing specific substrates for modification by ubiquitin (Ub). Separating genuine targets E3s from E3-interactors remains challenge. We present BioE3, powerful approach matching to Ub interest. Using BirA-E3 ligase fusions and bioUb, site-specific biotinylation Ub-modified particular facilitates proteomic identification. show that BioE3 identifies both known new two RING-type ligases: RNF4 (DNA damage response, PML bodies), MIB1...
Abstract The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation TurboID protein-fragment complementation to find SUMO-dependent interactors proteins interest. We develop an optimized split-TurboID version show SUMO interaction-dependent labelling proximal PML RANGAP1. are involved in transcription, DNA damage, stress response modification...
SUMOylation participates in ecdysteroid biosynthesis at the onset of metamorphosis Drosophila melanogaster. Silencing SUMO homologue smt3 prothoracic gland leads to reduced lipid content, low ecdysone titers, and a block larval–pupal transition. Here we show that SR-BI family Scavenger Receptors mediates functions. Reduced levels Snmp1 compromise uptake gland. In addition, overexpression is able recover droplet knockdown cells. expression depends on Ftz-f1 (an NR5A-type orphan nuclear...
Abstract Post-translational modification by ubiquitin and ubiquitin-like proteins (UbLs) is fundamental for maintaining protein homeostasis. Efficient isolation of UbL conjugates hampered multiple factors, including cost specificity reagents, removal UbLs proteases, distinguishing from interactors, low quantities modified substrates. Here we describe bioUbLs, a comprehensive set tools studying modifications in Drosophila mammals, based on multicistronic expression vivo biotinylation using...
Abstract Background The specificity of the ubiquitination process is mediated by E3 ligases. Discriminating genuine substrates E3s from mere interacting proteins one major challenges in field. We previously developed BioE3, a biotin-based approach that uses BirA-E3 fusions together with ubiquitin fused to low-affinity AviTag obtain site-specific and proximity-dependent biotinylation substrates. proved suitability BioE3 identify targets RING HECT-type Methods experiments were performed...
Abstract Background The specificity of the ubiquitination process is mediated by E3 ligases. Discriminating genuine substrates E3s from mere interacting proteins one major challenges in field. We previously developed BioE3, a biotin-based approach that uses BirA-E3 fusions together with ubiquitin fused to low-affinity AviTag obtain site-specific and proximity-dependent biotinylation substrates. proved suitability BioE3 identify targets RING HECT-type Methods experiments were performed...
Sumoylation, the covalent attachment of small ubiquitin-related modifier SUMO to target proteins, regulates different cellular processes, although its role in control development remains unclear. We studied sumoylation during Drosophila by using RNAi reduce smt3 mRNA levels specific tissues. knockdown prothoracic gland, which controls key developmental processes through synthesis and release ecdysteroids, caused a 4-fold prolongation larval life completely blocked transition from pupal...
In mammals, cholesterol is transformed into steroid hormones in the adrenal gland, ovaries or testes. The Scavenger Receptors Class B Type I (SR-BI) are membrane proteins that belong to CD36 family and participate selective uptake of high density lipoprotein cholesteryl ester mammalian steroidogenic tissues. Fourteen members have been identified Diptera, although their expression patterns remain uncharacterized. Using <I>in situ</I> hybridization we characterized fourteen SR-BIs...
Angelman syndrome is a complex neurodevelopmental disorder caused by the lack of function in brain single gene, UBE3A. The E3 ligase coded this gene known to build K48-linked ubiquitin chains, modification historically considered target substrates for degradation proteasome. However, change protein abundance not proof that candidate UBE3A substrate indeed ubiquitinated We have here used an unbiased proteomics approach, bioUb strategy, identify 79 proteins appear more Drosophila photoreceptor...
SUMOylation, the covalent binding of Small Ubiquitin-like Modifier (SUMO) to target proteins, is a posttranslational modification that regulates critical cellular processes in eukaryotes. In insects, SUMOylation has been studied holometabolous species, particularly dipteran Drosophila melanogaster, which contains single SUMO gene (smt3). This led assumption insects contain gene. However, analysis insect genomes shows basal two genes, orthologous vertebrate SUMO1 and SUMO2/3. Our...
The Spalt-like family of zinc finger transcription factors is conserved throughout evolution and involved in fundamental processes during development embryonic stem cell maintenance. Although human SALL1 modified by SUMO-1 vitro, it not known whether this post-translational modification plays a role regulating the activity factors. Here, we show that Drosophila Spalt are sumoylation. This influences their nuclear localization capacity to induce vein formation through regulation target genes...
Abstract Background Neurodegenerative diseases are progressive and irreversible they can be initiated by mutations in specific genes. Spalt-like genes ( Sall ) encode transcription factors expressed the central nervous system. In humans, SALL associated with hereditary syndromes characterized mental retardation, sensorineural deafness motoneuron problems, among others. Drosophila sall mutants exhibit severe neurodegeneration of system at embryonic stage 16, which surprisingly reverts later...
Development is orchestrated through a complex interplay of multiple transcription factors. The comprehension this will help us to understand developmental processes. Here we analyze the relationship between two key factors: CBX4, member Polycomb Repressive Complex 1 (PRC1), and SALL1, Spalt-like family with important roles in embryogenesis limb development. Both proteins localize nuclear bodies are modified by small ubiquitin-like modifier (SUMO). Our results show that CBX4 SALL1 interact...
ABSTRACT The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation TurboID protein-fragment complementation to find SUMO-dependent interactors proteins interest. We developed an optimized split-TurboID version show SUMO interaction-dependent labelling proximal PML RANGAP1. are involved in transcription, DNA damage, stress response...
ABSTRACT The post-translational modification of proteins by ubiquitination is a highly regulated process that involves dynamic, three-step enzymatic cascade, where more than 600 E3 ligases play critical role in recognizing specific substrates for modification. Separating bona fide targets E3s from E3-interacting remains major challenge the field. In this study, we present BioE3, novel approach identifying ubiquitin-like (UbL) interest. Using BirA-E3 ligase fusion and bioUbLs, method...