A5065638585- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Cancer-related Molecular Pathways
- Toxoplasma gondii Research Studies
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- Iron Metabolism and Disorders
- Immunotherapy and Immune Responses
- Biosimilars and Bioanalytical Methods
- T-cell and B-cell Immunology
- Autophagy in Disease and Therapy
- Histone Deacetylase Inhibitors Research
- Advanced Proteomics Techniques and Applications
- Streptococcal Infections and Treatments
- Protein Hydrolysis and Bioactive Peptides
- Seaweed-derived Bioactive Compounds
- Chemokine receptors and signaling
- Mosquito-borne diseases and control
- Phytoestrogen effects and research
- CAR-T cell therapy research
- Enzyme Catalysis and Immobilization
Universidad Nacional Autónoma de México
2000-2025
CIC bioGUNE
2013-2022
Instituto Politécnico Nacional
2014-2017
Instituto Nacional de Salud Pública
2016
Instituto de Salud Carlos III
2016
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2013
CIC biomaGUNE
2013
Abstract Binding and activation of human plasminogen (Plg) to generate the proteolytic enzyme plasmin (Plm) have been associated with invasive potential certain bacteria. In this work, proteomic analysis together ligand blotting assays identified several major Plg‐binding spots in Mycobacterium tuberculosis soluble extracts (SEs) culture filtrate proteins. The identity 15 different proteins was deduced by N‐terminal and/or MS corresponded DnaK, GroES, GlnA1, Ag85 complex, Mpt51, Mpt64, PrcB,...
A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 were identified mass spectrometry as putative glycoproteins 34 of them new probably mannosylated proteins. These results contribute to the construction affinity glycoprotein database M. tuberculosis, and provide useful information for understanding biological role in mycobacteria.
Abstract Post-translational modification by ubiquitin and ubiquitin-like proteins (UbLs) is fundamental for maintaining protein homeostasis. Efficient isolation of UbL conjugates hampered multiple factors, including cost specificity reagents, removal UbLs proteases, distinguishing from interactors, low quantities modified substrates. Here we describe bioUbLs, a comprehensive set tools studying modifications in Drosophila mammals, based on multicistronic expression vivo biotinylation using...
Resistance to bortezomib (BTZ) represents a major bottleneck continue using this proteasome inhibitor in the treatment of mantle cell lymphoma (MCL). In study, we investigated mechanisms by which TRIM24 (tripartite motif-containing 24), ubiquitin ligase enriched ubiquitome BTZ-resistant MCL cells, modulates proteasome-autophagy crosstalk. The localization and stability were differentially influenced inhibition or autophagy cells with acquired BTZ resistance (ZBR). Moreover, genetic deletion...
SUMO-modified proteins are recognized by SUMO interacting motifs (SIMs), thus triggering diverse cellular responses. Here SIMs were used to develop SUMO-traps capture endogenous SUMOylated proteins. Our results show that these small peptides transferable maintain their binding capacity when fused the heterologous carrier protein GST. The tandem disposition of increases specifically interact with polySUMO but not poly-Ubiquitin chains. We demonstrate this capturing system purifies such as...
Proteolysis Targeting Chimeras (PROTACs) describe compounds that bind to and induce degradation of a target by simultaneously binding ubiquitin ligase. More generally referred as bifunctional degraders, PROTACs have led the way in field targeted protein (TPD) with several currently undergoing clinical testing. Alongside single-moiety compounds, or molecular glue degraders (MGDs), are increasingly being considered viable approach for development therapeutics, driven advances rational...
The ubiquitin proteasome system (UPS) is one of the main proteolytical pathways in eukaryotic cells and plays an essential role key cellular processes such as cell cycle, stress response, signal transduction, transcriptional regulation. Many components this pathway have been implicated diverse pathologies including cancer, neurodegeneration infectious diseases, malaria. success inhibitors clinical trials underlines potential UPS drug discovery.
ABSTRACT The first evidence of the interaction Mycobacterium tuberculosis with plasminogen system is herein reported. By FACScan analysis and affinity blotting, lysine-dependent binding to M. was demonstrated. molecules were 30-, 60-, 66-kDa proteins present in cell wall soluble protein extracts. activation plasminogen, which occurred only presence fibrin not inhibited by host serpin, α 2 -antiplasmin, also
Deep Eutectic Solvents (DES) were investigated as new reaction media for the synthesis of alkyl glycosides catalyzed by thermostable α-amylase from Thermotoga maritima Amy A. The enzyme was almost completely deactivated when assayed in a series pure DES, but cosolvents, DES containing alcohols, sugars, and amides hydrogen-bond donors (HBD) performed best. A choline chloride:urea based further characterized alcoholysis using methanol nucleophile. As cosolvent, this increased hydrolytic...
Protein ubiquitylation coordinates crucial cellular events in physiological and pathological conditions. A comparative analysis of the ubiquitin proteome from bortezomib (BTZ)-sensitive BTZ-resistant mantle cell lymphoma (MCL) revealed an enrichment autophagy-lysosome system (ALS) cells. Pharmacological inhibition autophagy at level lysosome-fusion a constitutive activation proteaphagy accumulation proteasome subunits within autophagosomes different MCL lines with acquired or natural...
DNA damage activated by Adriamycin (ADR) promotes ubiquitin–proteasome system-mediated proteolysis stimulating both the activity of ubiquitylating enzymes and proteasome. In ADR-resistant breast cancer MCF7 (MCF7ADR) cells, protein ubiquitylation is significantly reduced compared to parental cells. Here, we used tandem ubiquitin-binding entities (TUBEs) analyze pattern observed in or MCF7ADR While MCF7, level total increased up six-fold response ADR, cells only a two-fold was found. To...
Abstract The mechanism by which glycoside hydrolases control the reaction specificity through hydrolysis or transglycosylation is a key element embedded in their chemical structures. determinants of seem to be complex. We looked for structural differences domain B between 4-α-glucanotransferase from Thermotoga maritima ( Tm GTase) and α-amylase petrophila (Tp Amylase ) found longer loop former that extends towards active site carrying W residue at its tip. Based on these we constructed...
Abstract The enzymatic production of alkyl glucosides is limited by the stability enzymes in presence alcohols. In present study, we investigated three different supports: Sepharose 4B, crosslinked (Fast Flow), and Eupergit C for immobilization α-amylase from Thermotoga maritima , AmyA, to increase its during alcoholysis reaction. enzyme immobilized on showed best results, allowing reutilization at least five cycles while maintaining more than 50% residual activity. addition, a previously...