A5006317237- Genetic and Kidney Cyst Diseases
- Autophagy in Disease and Therapy
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA regulation and disease
- Renal and related cancers
- Immune cells in cancer
- Amino Acid Enzymes and Metabolism
- Alzheimer's disease research and treatments
- Cerebrospinal fluid and hydrocephalus
- Protist diversity and phylogeny
- Neuroscience and Neuropharmacology Research
- Genomic variations and chromosomal abnormalities
- Educational Practices and Sociocultural Research
- Solar-Powered Water Purification Methods
- Drug Transport and Resistance Mechanisms
- Adenosine and Purinergic Signaling
- Renal cell carcinoma treatment
- Respiratory viral infections research
- Extracellular vesicles in disease
- Neurogenesis and neuroplasticity mechanisms
- Phagocytosis and Immune Regulation
- Advanced biosensing and bioanalysis techniques
University of the Basque Country
2009-2024
Achucarro Basque Center for Neuroscience
2016-2023
Institut des Maladies Neurodégénératives
2016-2021
Université de Bordeaux
2016-2021
Centre National de la Recherche Scientifique
2016-2021
Biomedical Research Networking Center on Neurodegenerative Diseases
2009-2016
Albert Einstein College of Medicine
2013-2014
Euskadiko Parke Teknologikoa
2008-2011
Microglial phagocytosis of apoptotic debris prevents buildup damage neighbor neurons and inflammatory responses. Whereas microglia are very competent phagocytes under physiological conditions, we report their dysfunction in mouse preclinical monkey models stroke (macaques marmosets) by transient occlusion the medial cerebral artery (tMCAo). By analyzing recently published bulk single cell RNA sequencing databases, show that was not explained transcriptional changes. In contrast, demonstrate...
Brain ischemia leading to stroke is a major cause of disability in developed countries. Therapeutic strategies have most commonly focused on protecting neurons from ischemic damage. However, damage white matter causes oligodendrocyte death, myelin disruption, and axon dysfunction, it partially mediated by glutamate excitotoxicity. We previously demonstrated that oligodendrocytes express ionotropic purinergic receptors. The objective this study was investigate the role signaling ischemia....
Abstract Background Glutamate excitotoxicity contributes to oligodendrocyte and tissue damage in multiple sclerosis (MS). Intriguingly, glutamate level plasma cerebrospinal fluid of MS patients is elevated, a feature which may be related the pathophysiology this disease. In addition transporters, levels extracellular are controlled by cystine/glutamate antiporter x c - , an exchanger that provides intracellular cystine for production glutathione, major cellular antioxidant. The objective...
Summary Inability to preserve proteostasis with age contributes the gradual loss of function that characterizes old organisms. Defective autophagy, a component network for delivery and degradation intracellular materials in lysosomes, has been described multiple organisms, while robust autophagy response linked longevity. The molecular mechanisms responsible defective autophagic remain, most part, poorly characterized. In this work, we have identified differences between young cells...
Primary cilia are sensory organelles crucial for cell signaling during development and organ homeostasis. Cilia arise from centrosomes their formation function is governed by numerous factors. Through our studies on Townes-Brocks Syndrome (TBS), a rare disease linked to abnormal in human fibroblasts, we uncovered the leucine-zipper protein LUZP1 as an interactor of truncated SALL1, dominantly-acting causing disease. Using TurboID proximity labeling pulldowns, show that associates with...
The cystine/glutamate antiporter is a membrane transport system responsible for the uptake of extracellular cystine and release intracellular glutamate. It major source in most cells, key regulator extrasynaptic glutamate CNS. Because limiting factor biosynthesis glutathione, abundant neurotransmitter, central player both antioxidant defense glutamatergic signaling, two events critical to brain function. However, distribution CNS has not been well characterized. Here, we analyzed expression...
Primary cilia are sensory membrane protrusions whose dysfunction causes ciliopathies. INPP5E is a ciliary phosphoinositide phosphatase mutated in ciliopathies like Joubert syndrome. regulates numerous functions, but how it accumulates remains poorly understood. Herein, we show targeting requires its folded catalytic domain and controlled by four conserved localization signals (CLSs): LLxPIR motif (CLS1), W383 (CLS2), FDRxLYL (CLS3) CaaX box (CLS4). We answer two long-standing questions the...
ABSTRACT The SARS-CoV-2 pandemic has evolved far more aggressively in countries lacking a robust testing strategy to identify infected individuals. Given the global demand for fast and reliable diagnosis determine carrier individuals, stock-out scenario number of essential reagents/kits used along diagnostic process been foreseen by many organizations. Having identified RNA extraction step as one key bottlenecks, we tested several alternatives that avoid use commercial kits this step....
ABSTRACT Primary cilia are sensory organelles that crucial for cell signaling during development and organ homeostasis. Cilia arise from the centrosome their formation is governed by numerous regulatory factors. We show leucine-zipper protein LUZP1 localizes to pericentriolar material actin cytoskeleton. Using TurboID proximity labeling pulldowns, associates with factors linked filaments. Loss of reduces F-actin levels, facilitating ciliogenesis altering Sonic Hedgehog signaling, pointing a...
Abstract Introduction SNPs associated with genome-wide risk for multiple sclerosis (MS) modulate expression of ankyrin repeat domain protein 55 (ANKRD55). The function ANKRD55 is not well understood. A role in ciliar transport multiciliated cells has been reported. To gain deeper insight how may neuro-inflammatory parameters, we identified the interactomes from human neuroblastoma, astrocytic, microglial and monocytic cell lines. Methods Cell lines were transfected synthetic RNA conjunction...
ABSTRACT The major neurodegenerative diseases, like Alzheimer’s disease (AD), accumulate neuropathogenic proteins that compromise autophagic function. In AD, autophagy contributes to intracellular APP processing and amyloid beta (Aβ) generation by mutant presenilin-1 (PS1). However, how extracellular soluble Aβ oligomers (Aβo) impact is not well understood. primary cilium (PC), a signaling organelle on the surface of mature neurons glia, able bind Aβ. Since PC pathways knowingly modify in...
ABSTRACT Primary cilia are microtubule-based signaling organelles present in the plasma membrane of most cell types, including mature astrocytes and neurons. However, little is known about role this organelle brain. Data from our lab show that neuronal primary (nPC) required for soluble amyloid beta oligomer modulation autophagy, these events age dependent. Here, we hypothesize react to loss nPC aging might impact events. For purpose, have characterized morphological changes as well cilium...
The Spanish Society for Developmental Biology (SEBD) organized its 17th meeting in November 2020 (herein referred to as SEBD2020). This meeting, originally programmed take place the city of Bilbao, was forced onto an online format due SARS-CoV2, COVID-19 pandemic. Although, we missed live personal interactions and out on Bilbao social scene, were able meet present our work discuss latest results. An overview activities that took around different scientific sessions speakers involved are...