A5031119579- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Genetic and Kidney Cyst Diseases
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Hedgehog Signaling Pathway Studies
- Cell death mechanisms and regulation
- Retinal Development and Disorders
- Protist diversity and phylogeny
- RNA Research and Splicing
- Plant Molecular Biology Research
- Experimental Learning in Engineering
- Genomic variations and chromosomal abnormalities
- Protein Kinase Regulation and GTPase Signaling
- Cancer, Hypoxia, and Metabolism
- S100 Proteins and Annexins
- Amino Acid Enzymes and Metabolism
- Biomedical Research and Pathophysiology
- Analog and Mixed-Signal Circuit Design
- Fetal and Pediatric Neurological Disorders
- Immunotherapy and Immune Responses
- Nuclear Structure and Function
- Advanced MEMS and NEMS Technologies
- Mechanisms of cancer metastasis
- Metabolism, Diabetes, and Cancer
Instituto de Investigaciones Biomédicas Sols-Morreale
2020-2024
Centre for Biomedical Network Research on Rare Diseases
2022-2023
Instituto de Salud Carlos III
2022-2023
Hospital Universitario La Paz
2020-2023
Consejo Superior de Investigaciones Científicas
2020-2023
Universidad Autónoma de Madrid
2020-2023
Instituto de Investigación de Enfermedades Raras
2022
Centro de Investigación Biomédica en Red
2022
Universidade de Santiago de Compostela
2010-2017
University of Buenos Aires
2011
G protein-coupled receptors (GPCRs) are the most common pharmacological target in human clinical practice. To perform their functions, many GPCRs must accumulate inside primary cilia, microtubule-based plasma membrane protrusions working as cellular antennae. Nevertheless, molecular mechanisms underlying GPCR ciliary targeting remain poorly understood. Serotonin receptor 6 (HTR6) and somatostatin 3 (SSTR3) two brain-enriched involved cognition pathologies such Alzheimer's disease cancer....
Primary cilia are sensory membrane protrusions whose dysfunction causes ciliopathies. INPP5E is a ciliary phosphoinositide phosphatase mutated in ciliopathies like Joubert syndrome. regulates numerous functions, but how it accumulates remains poorly understood. Herein, we show targeting requires its folded catalytic domain and controlled by four conserved localization signals (CLSs): LLxPIR motif (CLS1), W383 (CLS2), FDRxLYL (CLS3) CaaX box (CLS4). We answer two long-standing questions the...
We analyzed here how formin-like 1 β (FMNL1β), an actin cytoskeleton-regulatory protein, regulates microtubule-organizing center (MTOC) and multivesicular bodies (MVB) polarization exosome secretion at immune synapse (IS) model in a phosphorylation-dependent manner. IS formation was associated with transient recruitment of FMNL1β to the IS, which independent protein kinase C δ (PKCδ). Simultaneous RNA interference all FMNL1 isoforms prevented MTOC/MVB secretion, were restored by FMNL1βWT...
Phosphorylation of prothymosin α (ProTα) appears not to affect its influence on chromatin remodelling. To determine whether it affects nuclear import or cytosolic antiapoptotic activity, cells were transfected with vectors generating tagged recombinant ProTα (rProTα), either wild-type (rProTα-wt), which is partially phosphorylated posttranslation the nonphosphorylatable rProTα-T7A. Immunofluorescence microscopy showed predominant location native ProTα, rProTα-wt, and rProTα-T7A in nucleus....
Ellis van Creveld syndrome and Weyers acrofacial dysostosis are two rare genetic diseases affecting skeletal development. They both ciliopathies, as they due to malfunction of primary cilia, microtubule-based plasma membrane protrusions that function cellular antennae required for Hedgehog signaling, a key pathway during morphogenesis. These ciliopathies caused by mutations the EVC-EVC2 complex, transmembrane protein heterodimer regulates signaling from inside cilia. Despite importance this...
ABSTRACT G protein-coupled receptors (GPCRs) are the most common pharmacological target in clinical practice. To perform their signaling functions, many GPCRs must accumulate at primary cilia, microtubule-based plasma membrane protrusions that work as cellular antennae. Despite great importance, molecular mechanisms underlying GPCR ciliary targeting remain poorly understood. Serotonin receptor 6 (Htr6) and somatostatin 3 (Sstr3) two brain-enriched controlling cognition involved multiple...
<title>Abstract</title> T-cell receptor stimulation by antigen bound to the major histocompatibility complex (MHC) on an antigen-presenting cell (APC) induces protein kinase C (PKC) activation and formation of immune synapse (IS), followed depletion filamentous actin (F-actin) at central region IS (cIS) polarization multivesicular bodies (MVB) microtubule-organizing center (MTOC) IS. These events lead polarized exosome secretion exosomes are involved in several crucial responses such as...
T-cell receptor stimulation by antigen bound to the major histocompatibility complex (MHC) on an antigen-presenting cell (APC) induces protein kinase C (PKC) activation and formation of immune synapse (IS), followed depletion filamentous actin (F-actin) at central region IS (cIS) polarization multivesicular bodies (MVB) microtubule-organizing center (MTOC) IS. These events lead polarized exosome secretion exosomes are involved in several crucial responses such as autocrine activation-induced...
<title>Abstract</title> T-cell receptor stimulation (TCR) by antigen bound to the major histocompatibility complex (MHC) on an antigen-presenting cell (APC) induces protein kinase C (PKC) activation and formation of immune synapse (IS), followed depletion filamentous actin (F-actin) at central region IS (cIS) polarization multivesicular bodies (MVB) microtubule-organizing center (MTOC) IS. These events lead polarized exosome secretion exosomes are involved in several crucial responses such...
<title>Abstract</title> T-cell receptor stimulation (TCR) by antigen bound to the major histocompatibility complex (MHC) on an antigen-presenting cell (APC) induces protein kinase C (PKC) activation and formation of immune synapse (IS), followed depletion filamentous actin (F-actin) at central region IS (cIS) polarization multivesicular bodies (MVB) microtubule-organizing center (MTOC) IS. These events lead polarized exosome secretion exosomes are involved in several crucial responses such...
<title>Abstract</title> T-cell receptor stimulation (TCR) by antigen bound to the major histocompatibility complex (MHC) on an antigen-presenting cell (APC) induces protein kinase C (PKC) activation and formation of immune synapse (IS), followed depletion filamentous actin (F-actin) at central region IS (cIS) polarization multivesicular bodies (MVB) microtubule-organizing center (MTOC) IS. These events lead polarized exosome secretion exosomes are involved in several crucial responses such...
T-cell receptor stimulation (TCR) by antigen bound to the major histocompatibility complex (MHC) on an antigen-presenting cell (APC) induces protein kinase C (PKC) activation and formation of immune synapse (IS), followed depletion filamentous actin (F-actin) at central region IS (cIS) polarization multivesicular bodies (MVB) microtubule-organizing center (MTOC) IS. These events lead polarized exosome secretion exosomes are involved in several crucial responses such as autocrine...
T-cell receptor stimulation (TCR) by antigen bound to the major histocompatibility complex (MHC) on an antigen-presenting cell (APC) induces protein kinase C (PKC) activation and formation of immune synapse (IS), followed depletion filamentous actin (F-actin) at central region IS (cIS) polarization multivesicular bodies (MVB) microtubule-organizing center (MTOC) IS. These events lead polarized exosome secretion exosomes are involved in several crucial responses such as autocrine...
We analyzed here how formin-like 1 β (FMNL1β), an actin cytoskeleton-regulatory protein, regulates microtubule-organizing center (MTOC) and multivesicular bodies (MVB) polarization exosome secretion at immune synapse (IS) model in a phosphorylation-dependent manner. IS formation was associated with transient recruitment of FMNL1β to the IS, which independent protein kinase C δ (PKCδ). Simultaneous RNA interference all FMNL1 isoforms prevented MTOC/MVB secretion, were restored by FMNL1βWT...
This work presents the development and first experiences in a course of design digital analog integrated circuits using CMOS process country university which do not have strong traditions on microelectronics. During two months course, lectures are given specific topics, leaving last to project proposed by students themselves, is fabricated. The ends when characterize functionality their circuits.
<title>Abstract</title> T-cell receptor stimulation by antigen bound to the major histocompatibility complex (MHC) on an antigen-presenting cell (APC) induces protein kinase C (PKC) activation and formation of immune synapse (IS), followed depletion filamentous actin (F-actin) at central region IS (cIS) polarization multivesicular bodies (MVB) microtubule-organizing center (MTOC) IS. These events lead polarized exosome secretion exosomes are involved in several crucial responses such as...