A5029946829- Extracellular vesicles in disease
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
- Chemical Reactions and Isotopes
- Advanced Fluorescence Microscopy Techniques
- Animal Disease Management and Epidemiology
- NF-κB Signaling Pathways
- Ubiquitin and proteasome pathways
- Erythrocyte Function and Pathophysiology
- Wastewater Treatment and Nitrogen Removal
- Genomics, phytochemicals, and oxidative stress
- Toxin Mechanisms and Immunotoxins
- Melanoma and MAPK Pathways
- Adenosine and Purinergic Signaling
- Protein Kinase Regulation and GTPase Signaling
- Enzyme function and inhibition
- MicroRNA in disease regulation
- Cell Image Analysis Techniques
Universidad Autónoma de Madrid
2007-2024
Instituto de Investigaciones Biomédicas Sols-Morreale
1996-2024
Hospital Universitario Puerta de Hierro Majadahonda
2023
Consejo Superior de Investigaciones Científicas
1990-2021
Hospital Universitario de La Princesa
1997
Centro de Biología Molecular Severo Ochoa
1990-1994
Dana-Farber Cancer Institute
1992-1994
Zero to Three
1994
Brigham and Women's Hospital
1992
Harvard University Press
1992
The immunosuppressant rapamycin inhibited proliferation of the H4IIEC hepatoma cell line. Rapamycin, but not its structural analog FK506, also basal and insulin-stimulated activity p70 ribosomal protein S6 kinase. By contrast, insulin stimulation p85 Rsk kinase mitogen-activated (MAP) were unaffected by drug. Rapamycin treatment COS cells transfected with recombinant completely appearance hyperphosphorylated form concomitant inhibition enzyme toward 40 S subunits. Thus, inhibits a signal...
Binding of interleukin 2 (IL-2) to its receptor generates intracellular signals, including the activation tyrosine and serine/threonine kinases. In this study serine/threonine-specific ribosomal protein S6 kinases in response IL-2 was analyzed murine T-cell line CTLL-20, a model system IL-2-dependent proliferation. Two major classes have been characterized: 90-kDa (rsk) family 70-kDa family. addition recombinant IL-2, total kinase activity increased. This could not be immunoprecipitated by...
Herpes simplex virus 1 (HSV-1) is a neurotropic pathogen that can infect many types of cells and establishes latent infections in the neurons sensory ganglia. In some cases, spreads into central nervous system, causing encephalitis or meningitis. Cells infected with several different viruses may secrete microvesicles (MVs) containing viral proteins RNAs. instances, extracellular harboring infectious have been found. Here we describe features shedding released by human oligodendroglial HOG...
Patients with lung cancer may suffer from sexual dysfunction (SD) related to oncological treatment. This is an under-recognized condition among clinicians. The aim of this study was describe the prevalence SD in a multicenter cohort patients. multicenter, cross-sectional, observational conducted between July 2023 and February 2024. Sexual function assessed by patient-reported outcome (PRO) system using sex-specific questionnaire. Descriptive analysis evaluation differences categorical...
We report here for the first time detection of ribosomal p70S6 kinase (p70S6K) in a hematopoietic cell, neutrophil, and stimulation its enzymatic activity by granulocyte macrophage colony-stimulating factor (GM-CSF). GM-CSF modified V max enzyme (from 7.2 to 20.5 pmol/min/mg) induced time- dose-dependent phosphorylation on p70S6K residues Thr389 Thr421/Ser424. The immunosuppressant macrolide rapamycin caused either decrease intensity phospho-Thr389 bands Western blots, or as downshift...
HLA-B27 subtype polymorphism is amenable to differential recognition by CTL. Site-directed mutagenesis was used construct a series of mutants reproducing most the changes occurring in natural subtypes. The reactivity 21 anti-HLA-B27 CTL clones examined with these address three issues concerning alloreactive response against HLA-B27: 1) diversity clonotypic specificities, 2) structural features epitopes recognized clones, and 3) role individual positions Virtually all displayed unique...
ABSTRACT T‐cell receptor stimulation induces the convergence of multivesicular bodies towards microtubule‐organizing centre (MTOC) and polarization MTOC to immune synapse (IS). These events lead exosome secretion at IS. We describe here that upon IS formation centrosomal area F‐actin decreased concomitantly with PKCδ‐interfered T cell clones showed a sustained level associated defective polarization. analysed contribution two actin cytoskeleton‐regulatory proteins, FMNL1 paxillin, regulation...
Multivesicular bodies (MVB) are endocytic compartments that enclose intraluminal vesicles (ILV) formed by inward budding from the limiting membrane of endosomes. In T lymphocytes, ILV secreted as Fas ligand-bearing, pro-apoptotic exosomes following cell receptor (TCR)-induced fusion MVB with plasma at immune synapse (IS). this study we show protein kinase C δ (PKCδ), a novel PKC isotype activated diacylglycerol (DAG), regulates TCR-controlled, polarization towards IS and exosome secretion....
AP-1 has been shown to behave as a redox-sensitive transcription factor that can be activated by both oxidant and antioxidant stimuli. However, the mechanisms involved in activation of antioxidants are largely unknown. In this study we show structurally unrelated agents pyrrolidine dithiocarbamate (PDTC), butylated hydroxyanisole, Nacetylcysteine JNK (c-Jun NH2-terminal kinase) Jurkat T cells. This differed substantially from mediated phorbol 12-myristate 13-acetate (PMA) Ca2+ ionophore or...
Abstract Mononuclear cells from subjects infected with human T lymphotrophic virus type I (HTLV-I) display a unique ability to proliferate in vitro the absence of mitogens or exogenous growth factors. Subjects who have developed an HTLV-I-associated myelopathy (HAM) show even higher degree spontaneous proliferation concomitant transcription HTLV-I provirus. The mechanism underlying HTLV-I-induced cell activation was investigated by characterizing series HTLV-I-infected clones generated blood...
The T-cell glycoprotein CD2 not only contributes to intercellular adhesion but also plays a direct role in activation. Here we demonstrate that the interaction of with its ligand lymphocyte function-associated antigen 3 (CD58) is regulated by receptor-CD3 signaling. crosslinking specific or monoclonal antibodies rapidly increases avidity which cell-surface binds immunoaffinity-purified CD58. Mutational analysis cytoplasmic domain demonstrates carboxyl-terminal asparagine essential for...
Abstract The cytokines interleukin (IL)‐3 and erythropoietin (EPO) are critical regulators of the proliferation differentiation cells hematopoietic system, but their intracellular mechanisms action not fully understood. Binding IL‐3 to receptor (IL‐3R) binding EPO EPOR both induce changes in tyrosine serine/threonine phosphorylation; phosphorylation a number polypeptides appears be shared response upon cytokine stimulation. We have previously shown that IL‐2 IL‐2R activates 70‐kDa (p70) S6...
A group of cross-hybridizing DNA segments contained within the restriction fragments RK', RL, RJ, and RD' African swine fever virus were mapped sequenced. Analysis these sequences revealed presence a family homologous open reading frames in regions close to ends. The whole is composed six with an average length 360 coding triplets (multigene 360), four which are located left part genome two right terminal EcoRI fragment. In proximity inverted repeat, we found additional small frame was...