Metal-organic frameworks (MOFs) are among the most attractive porous materials available today. They have garnered much attention for their potential utility in many different areas such as gas storage, separation, catalysis, and biomedicine. However, very little is known about possible health or environmental risks of these materials. Here, results toxicity studies on sixteen representative uncoated MOF nanoparticles (nanoMOFs), which were assessed cytotoxicity to HepG2 MCF7 cells vitro,...

Dopamine (DA) is one of the main neurotransmitters found in central nervous system and has a vital role function dopaminergic (DArgic) neurons. A progressive loss this specific subset cells hallmarks age-related neurodegenerative disorders such as Parkinson's disease (PD). Symptomatic therapy for PD been centered precursor l-DOPA administration, an amino acid DA that crosses blood-brain barrier (BBB) while does not, although approach presents medium- to long-term side effects. To overcome...

The present work faces the rising demand of cationic particles different sizes for biological applications, especially in gene therapies and nanotoxicology studies. A simple phase-transfer methodology has been developed functionalization gold nanoparticles (Au NPs) with a variety ligands, both anionic aqueous solution, employing nanocrystal narrow size distributions. Successful demonstrated by UV-vis spectroscopy, DLS, ζ-potential, FTIR spectroscopy characterization before after phase...

Four cationic ruthenium(II) complexes with the formula [Ru(eta(5)-C(5)H(5))(PPh(3))(2)](+), L = 5-phenyl-1H-tetrazole (TzH) 1, imidazole (ImH) 2, benzo[1,2-b;4,3-b'] dithio-phen-2-carbonitrile (Bzt) 3, and [5-(2-thiophen-2-yl)-vinyl]-thiophene-2-carbonitrile] (Tvt) 4 were prepared characterized in view to evaluate their potentialities as antitumor agents. Studies by Circular Dichroism indicated changes secondary structure of ct-DNA. Changes tertiary pBR322 plasmid DNA also observed gel...

Four complexes combining the {Ru(p-cym)} moiety (p-cym = para-cymene) with thiosemicarbazone (TSC) ligands containing 5-nitrofuryl pharmacophore were investigated in vitro for their properties as prospective anti-tumour agents. The compounds are dimeric structures of general formula [Ru2(p-cym)2(L)2]X2 where X Cl(-), PF6(-) and L deprotonated 5-nitrofuraldehyde TSC (L1), N-methyl (L2), N-ethyl (L3) N-phenyl (L4) derivatives. precursor [RuCl2(p-cym)]2, all L1-L4and corresponding 1-4 screened...

The posttranslational modification of carboxy-terminal tails tubulin plays an important role in the regulation microtubule cytoskeleton. Enzymes responsible for deglutamylating have been discovered within a novel family mammalian cytosolic carboxypeptidases. discovery these enzymes also revealed existence range other substrates that are enzymatically deglutamylated. Only four six carboxypeptidases had characterized. Here we complete functional characterization this protein by demonstrating...

In the last years, extensive attention has been paid on designing and developing functional imaging contrast agents for providing accurate noninvasive evaluation of pathology in vivo. However, issue false-positives or ambiguous lack a robust strategy simultaneous dual-mode remain to be fully addressed. One effective improving it is rationally design magnetic resonance (MRI) (CAs) with intrinsic T1/ T2 features. this work, development characterization one-pot synthesized nanostructured...

Three novel dinuclear Cu(II) complexes based on a N,N,O-chelating salphen-like ligand scaffold and bearing varying aromatic substituents (−H, −Cl, −Br) have been synthesized characterized. The experimental computational data obtained suggest that all three exist in the dimeric form solid state adopt same conformation. mass spectrometry electron paramagnetic resonance results indicate structure coexists with monomeric solution upon solvent (dimethyl sulfoxide water) coordination. exhibit high...

Palladium and platinum complexes with the model nucleobase 1-methylcytosine (1-Mecyt) of types [Pd(N−N)(C6F5)(1-Mecyt)]ClO4 [N−N = bis(3,5-dimethylpyrazol-1-yl)methane (bpzm*), bis(pyrazol-1-yl)methane (bpzm), N,N,N',N'-tetramethylethylenediamine (tmeda), or 2,2'-bipyridine (bpy)] [M(dmba)(L')(1-Mecyt)]ClO4 [dmba N,C-chelating 2-(dimethylaminomethyl)phenyl; L' PPh3 (M Pd Pt), DMSO Pt)] have been obtained. cis-[M(C6F5)2(1-Mecyt)2] Pt) cis-[Pd(L')(C6F5)(1-Mecyt)2]ClO4 (L' t-BuNC) also...

Nna1 has some sequence similarity to metallocarboxypeptidases, but the biochemical characterization of not previously been reported. In this work we performed a detailed genomic scan and found >100 homologues in bacteria, Protista, Animalia, including several paralogs most eukaryotic species. Phylogenetic analysis Nna1-like sequences demonstrates major divergence between peptidases known metallocarboxypeptidases subfamilies: M14A, M14B, M14C. Conformational modeling representative proteins...

The new complexes [Pd(dmba)(N10-9AA)(PPh3)]ClO4 (1), [Pt(dmba)(N9-9AA)(PPh3)]ClO4 (2), [Pd(dmba)(N10-9AA)Cl] (3), and [Pd(C6F5)(N10-9AA)(PPh3)Cl] (4) (9-AA = 9-aminoacridine; dmba N,C-chelating 2-(dimethylaminomethyl)phenyl) have been prepared. crystal structures established by X-ray diffraction. In complex 2, an anagostic C−H···Pt interaction is observed. All are luminescent in the solid state at room temperature, showing important differences between palladium platinum complexes. Complex 2...

Pd(II) and Pt(II) complexes with the anions of model nucleobases 1-methylthymine (1-MethyH), 1-methyluracil (1-MeuraH), 1-methylcytosine (1-MecytH) types [Pd(dmba)(μ-L)]2 [dmba = N,C-chelating 2-((dimethylamino)methyl)phenyl; L 1-Methy, 1-Meura or 1-Mecyt] [M(dmba)(L)(L')] [L 1-Methy 1-Meura; L' PPh3 (M Pd Pt), DMSO Pt)] have been obtained. Palladium [Pd(C6F5)(N−N)(L)] N−N N,N,N',N'-tetramethylethylenediamine (tmeda), 2,2'-bipyridine (bpy), 4,4'-dimethyl-2,2'-bipyridine (Me2bpy)]...

The macrocyclic ligand DOTP is used to assemble a porous, heterometallic metal–organic framework (MOF). This MOF miniaturizable down the nanoscale form stable colloids, in physiological saline solution and cell culture media, not cytotoxic. It shows interesting relaxometric properties with r1 at high field (500 MHz) of 5 mM–1·s–1 maximum = 15 40 MHz, which remains constant over wide pH range increases temperature.

CPA4 (carboxypeptidase A4) is a member of the metallocarboxypeptidase family. was originally found in screen mRNAs up-regulated by sodium butyrate-induced differentiation cancer cells. Further studies suggested relation between and prostate aggressiveness. In present study, we determined that secreted from cells as soluble proenzyme (pro-CPA4) can be activated endoproteases, such trypsin. Three complementary approaches were used to study substrate specificity CPA4; kinetic analysis performed...

A novel proteinaceous metallo-carboxypeptidase inhibitor, named tick carboxypeptidase inhibitor (TCI), was isolated from the ixodid Rhipicephalus bursa and N-terminally sequenced. The complete cDNA encoding this protein cloned mRNA by reverse transcription-PCR rapid amplification of ends techniques. full-length TCI contains an open reading frame coding for a precursor 97 amino acid residues that consists predicted signal peptide 22 mature TCI, 75-residue cysteine-rich (12 Cys). deduced...

Abstract Functionalization of nanoparticles can significantly influence their properties and potential applications. Although researchers now functionalize metal, metal oxide, organic polymer with a high degree precision, controlled surface functionalization nanoscale coordination particles (CPPs) has remained significant challenge. The lack methodology is perhaps one the greatest roadblocks to advancement CPPs into added‐value drug delivery Here, we report having achieved this goal through...

Through processing peptide and protein C termini, carboxypeptidases participate in the regulation of various biological processes. Few tools are however available to study substrate specificity profiles these enzymes. We developed a proteome-derived library approach preferences carboxypeptidases. Our COFRADIC-based takes advantage distinct chromatographic behavior intact peptides proteolytic products generated by action carboxypeptidases, enrich latter facilitate its MS-based identification....