A5044418949- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- BRCA gene mutations in cancer
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Signaling Pathways in Disease
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Genomic variations and chromosomal abnormalities
- Peptidase Inhibition and Analysis
- Micro and Nano Robotics
- Endoplasmic Reticulum Stress and Disease
- Genetic factors in colorectal cancer
Universidade Nova de Lisboa
2014-2019
Instituto Gulbenkian de Ciência
2003-2017
University of Georgia
2010
IPO Porto
2002
Arl13b belongs to the ADP-ribosylation factor family within Ras superfamily of regulatory GTPases. Mutations in cause Joubert syndrome, which is characterized by congenital cerebellar ataxia, hypotonia, oculomotor apraxia, and mental retardation. highly enriched cilia required for ciliogenesis multiple organs. Nevertheless, precise role remains elusive. Here we report that exocyst subunits Sec8, Exo70, Sec5 bind preferentially GTP-bound form Arl13b, consistent with being an effector Arl13b....
The posttranslational modification of carboxy-terminal tails tubulin plays an important role in the regulation microtubule cytoskeleton. Enzymes responsible for deglutamylating have been discovered within a novel family mammalian cytosolic carboxypeptidases. discovery these enzymes also revealed existence range other substrates that are enzymatically deglutamylated. Only four six carboxypeptidases had characterized. Here we complete functional characterization this protein by demonstrating...
Article21 August 2019Open Access Source DataTransparent process Rab35 controls cilium length, function and membrane composition Stefanie Kuhns Corresponding Author [email protected] orcid.org/0000-0002-3065-7818 School of Biomolecular Biomedical Science, University College Dublin, Dublin 4, Ireland Department Biochemistry Molecular Biology, Southern Denmark, Odense M, Denmark Search for more papers by this author Cecília Seixas orcid.org/0000-0002-7364-4320 CEDOC, NOVA Medical...
The Arf-like protein Arl13b has been implicated in ciliogenesis and Sonic hedgehog signaling. Furthermore, we have previously shown that it regulates endocytic recycling traffic interacts with actin. Herein, report the non-muscle myosin heavy chain IIA, also known as Myh9, is an effector. Moreover, found both proteins localized to circular dorsal ruffles (CDRs) induced by platelet-derived growth factor stimulation are required for their formation. CDRs ring-shaped actin-dependent structures...
Background The eukaryotic cytosolic chaperonin CCT is a hetero-oligomeric complex formed by two rings connected back-to-back, each composed of eight distinct subunits (CCTα to CCTζ). mediates the folding, wide range newly synthesised proteins including tubulin (α, β and γ) actin, as quantitatively major substrates. Methodology/Principal Findings We disrupted genes encoding CCTα CCTδ in ciliate Tetrahymena. Cells lacking zygotic expression either or showed loss cell body microtubules, failed...
Methods We used the zebrafish Kupffer’s vesicle as a dynamic ciliary growth system and performed seven hour timecourse experiment comparing length of cilia measured by Arl13b-GFP or acetylated a-tubulin. In order to evaluate specificity alterations in a-tubulin acetylation pattern, we overexpressed different proteins that were also reported increase length.