A5014422195- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Renal and related cancers
- Cellular transport and secretion
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Micro and Nano Robotics
- Congenital heart defects research
- Assisted Reproductive Technology and Twin Pregnancy
- Acute Kidney Injury Research
- Paraoxonase enzyme and polymorphisms
- Fermentation and Sensory Analysis
- Horticultural and Viticultural Research
- RNA Interference and Gene Delivery
- Birth, Development, and Health
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
- Circular RNAs in diseases
- Cell Adhesion Molecules Research
- Chronic Kidney Disease and Diabetes
Universidade Nova de Lisboa
2014-2022
University of Lisbon
2017
Universidade Católica Portuguesa
2009
Exosomes are extracellular vesicles of endosomal origin that released by practically all cell types across metazoans. active vehicles intercellular communication and can transfer lipids, RNAs, proteins between different cells, tissues, or organs. Here, we describe a mechanism whereby containing KFERQ motif pentapeptide loaded into subpopulation exosomes in process is dependent on the membrane protein LAMP2A. Moreover, demonstrate this independent ESCRT machinery but HSC70, CD63, Alix,...
The Arf-like protein Arl13b has been implicated in ciliogenesis and Sonic hedgehog signaling. Furthermore, we have previously shown that it regulates endocytic recycling traffic interacts with actin. Herein, report the non-muscle myosin heavy chain IIA, also known as Myh9, is an effector. Moreover, found both proteins localized to circular dorsal ruffles (CDRs) induced by platelet-derived growth factor stimulation are required for their formation. CDRs ring-shaped actin-dependent structures...
Reduction in the length of motile cilia zebrafish left–right organizer (LRO), also known as Kupffer's vesicle, has a large impact on development. Here we demonstrate through genetic overexpression embryos and mathematical modelling that increased embryonic LRO fluid flow is milder than short cilia. Through Arl13b overexpression, which increases without impacting beat frequency, show increase cilium associated with decrease amplitude, resulting similar strengths for wild-type (WT) embryos,...
Methods We used the zebrafish Kupffer’s vesicle as a dynamic ciliary growth system and performed seven hour timecourse experiment comparing length of cilia measured by Arl13b-GFP or acetylated a-tubulin. In order to evaluate specificity alterations in a-tubulin acetylation pattern, we overexpressed different proteins that were also reported increase length.
Background Using the zebrafish mutant for deltaD gene (dld), it was shown involvement of Notch signaling in control cilia length cells fish laterality organ (Kupffer’s Vesicle, KV) [1]. Further research based on KV specific microarray screening allowed discovery several genes with differential expression. Of these, 23% were associated ciliogenesis and upon analysis, many proved to be involved cellular trafficking. Rabconnectin3a or rbcn3a strongly downregulated dld cells. Homologs this have...
Joubert Syndrome is a ciliopathy that can be caused by mutation in Arl13b protein. This syndrome characterized problems embryonic development, especially at the neurological level. protein belongs to small GTPase family, but presents double size of normal GTPase, because it has different C-terminus with coiled-coil domain and proline rich region. It known localizes cilium recent data showed ciliary membrane. However molecular function unknown. work based on functional study this where we...
Recently, using the zebrafish mutant for deltaD gene (after eight or aei-/-), our group has showed that Notch signaling was involved in control of cilia length cells fish laterality organ, Kupffer's Vesicle (KV) (Lopes el al. 2010).Further research based on microarray screening allowed discovery several genes with differential expression KV from aei-/mutants compared to WT embryos.One these genes, rabconnectin3 rc3 (ENSDARG00000091293) found be severely downregulated.Homologs this have...
Abstract Exosomes are extracellular vesicles of endosomal origin released by virtually all cell types across metazoans. active vehicles intercellular communication and can transfer lipids, RNAs proteins between different cells, tissues or organs. However, the mechanisms that regulate selective loading cytosolic into these still largely unknow. Here we describe a mechanism whereby containing pentapeptide sequence, biochemically related to KFERQ-motif, loaded subpopulation exosomes in process...