A5058622754- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Sulfur Compounds in Biology
- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- Pneumocystis jirovecii pneumonia detection and treatment
- Paraoxonase enzyme and polymorphisms
- Neuroscience of respiration and sleep
- Obstructive Sleep Apnea Research
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- HIV-related health complications and treatments
- Epigenetics and DNA Methylation
- Dialysis and Renal Disease Management
- Biochemical Acid Research Studies
- Folate and B Vitamins Research
- Pharmacological Effects and Toxicity Studies
- Hepatitis C virus research
- Angiogenesis and VEGF in Cancer
- Eicosanoids and Hypertension Pharmacology
- Parathyroid Disorders and Treatments
- Biochemical and Molecular Research
- Hormonal Regulation and Hypertension
- Adipose Tissue and Metabolism
- Drug-Induced Adverse Reactions
Universidade Nova de Lisboa
2016-2025
Centro Hospitalar de Lisboa Central
2006-2023
Centro Hospitalar Tondela-Viseu
2023
Pro Persona
2022
Centro Hospitalar de Vila Nova de Gaia
2022
Instituto Politécnico de Santarém
2022
Nova Medical (United States)
2015-2021
Centre for Chronic Disease Control
2019
Ovarian Cancer Action
2019
Imperial College London
2019
Abstract Ovarian cancer is the second most common gynaecologic malignancy and main cause of death from cancer, due to late diagnosis chemoresistance. Studies have reported role cysteine in by contributing for hydrogen sulphide (H 2 S) generation as a precursor glutathione (GSH). However, adaptation hypoxia therapy response remains unclear. We used several ovarian cell lines, ES2, OVCAR3, OVCAR8, A2780 A2780cisR, clarify relevance cells survival upon carboplatin. Results show that ES2 OVCAR8...
Chemoresistance to platinum-based antineoplastic agents is a consistent feature among ovarian carcinomas; however, whereas high-grade serous carcinoma (OSC) acquires resistance during chemotherapy, clear cell (OCCC) intrinsically resistant. The main objective of this study was explore, in vitro and vivo, if hepatocyte nuclear factor 1β (HNF1β) glutaminolysis contribute for the OCCC carboplatin through increased GSH bioavailability. To disclose role HNF1β, experiments were also performed an...
Bone marrow contains endothelial progenitor cells (EPCs) that, upon pro-angiogenic stimuli, migrate and differentiate into (ECs) contribute to re-endothelialization neo-vascularization. There are currently no reliable markers characterize EPCs, leading their inaccurate identification. In the past, we showed in a panel of tumors, some on vessel wall co-expressed CD14 (monocytic marker) CD31 (EC marker), indicating putative differentiation route monocytes ECs. Herein, disclosed as potential...
The diagnosis of kidney diseases presents significant challenges, including the reliance on variable and unstable biomarkers necessity for complex expensive laboratory tests. Raman spectroscopy emerges as a promising technique analyzing fluids, like urine, detecting important disease biomarkers. However, its complexity, high cost limited accessibility outside clinical contexts complicate application. Moreover, analysis spectra is challenging intensive task. In response to these in this...
Ovarian cancer is the main cause of death from gynecological cancer, with its poor prognosis mainly related to late diagnosis and chemoresistance (acquired or intrinsic) conventional alkylating reactive oxygen species (ROS)-generating drugs. We others reported that availability cysteine glutathione (GSH) impacts mechanisms resistance carboplatin in ovarian cancer. Different players metabolism can be crucial chemoresistance, such as cystine/glutamate antiporter system Xc (xCT)...
Abstract Chronic intermittent hypoxia (CIH) is the dominant pathological feature of human obstructive sleep apnoea (OSA), which highly prevalent and associated with cardiovascular renal diseases. CIH causes hypertension, centred on sympathetic nervous overactivity, persists following removal stimulus. Molecular mechanisms contributing to CIH‐induced hypertension have been carefully delineated. However, there a dearth knowledge efficacy interventions ameliorate high blood pressure in...
Background: Childhood overweight and obesity are increasing all over the world have been associated with low levels of physical activity (PA).Aims: To determine prevalence overweight, PA in Azorean children according to age sex; association between obesity.Subjects methods: Weight, height were measured 3699 aged 6–10 years, from Azores Islands, Portugal. Overweight classified cut-offs .Results: In girls, prevalences 22.8% 13.2%, boys 17.6% 12.3%, respectively. No trends found or obesity;...
Over the last decades, silent-killer carbon monoxide (CO) has been shown to also be an endogenous cytoprotective molecule able inhibit cell death and modulate mitochondrial metabolism. Neuronal metabolism is mostly oxidative neurons use glucose for maintaining their anti-oxidant status by generation of reduced glutathione (GSH) via pentose-phosphate pathway (PPP). It established that neuronal differentiation depends on reactive oxygen species (ROS) signalling, however there a lack...
The activation of endothelial cells (ECs) is a crucial step on the road map tumor angiogenesis and expanding evidence indicates that pro-oxidant microenvironment, conditioned by cancer metabolic rewiring, relevant controller this process. Herein, we investigated contribution oxidative stress-induced ferroptosis to ECs activation. Moreover, also addressed anti-angiogenic effect Propranolol. We observed ferroptosis-like mechanism, induced xCT inhibition with Erastin, at non-lethal level,...
Abacavir is a nucleoside reverse transcriptase inhibitor marketed since 1999 for the treatment of infection with human immunodeficiency virus type 1 (HIV). Despite its clinical efficacy, abacavir administration has been associated serious and sometimes fatal toxic events. reported to undergo bioactivation in vitro, yielding reactive species that bind covalently serum albumin, but haptenation mechanism significance events induced by this anti-HIV drug have yet be elucidated. extensively...
Nevirapine is widely used for the treatment of HIV-1 infection; however, its chronic use has been associated with severe liver and skin toxicity. Women are at increased risk these toxic events, but reasons sex-related differences unclear. Disparities in biotransformation nevirapine generation metabolites between men women might be underlying cause. The present work aimed to explore sex as a potential factor nevirapine-induced All included subjects were adults who had receiving 400 mg once...
Ovarian cancer is the second most common gynaecologic malignancy and cause of death from cancer, especially due to diagnosis at an advanced stage, when a cure rare. As ovarian tumour grows, cells are exposed regions hypoxia. Hypoxia known be partially responsible for progression, metastasis resistance therapies. These suggest that hypoxia entails selective pressure in which adapted not only have fitness increase under environment, but also non-selective adverse environments. In here, we used...
Among gynecologic malignancies, ovarian cancer is the third most prevalent and common cause of death, especially due to diagnosis at an advanced stage together with resistance therapy. As a solid tumor grows, cells in microenvironment are exposed regions hypoxia, selective pressure prompting progression chemoresistance. We have previously shown that cysteine contributes adaptation this hypoxic microenvironment, but mechanisms by which protects from hypoxia-induced death still be unveiled....
AbstractObjective. To investigate Portuguese children's health related physical fitness (HRPF) pass rates, and to analyze the associations between HRPF sex, age, activity, overweight/obesity gross motor coordination. Methods. Weight, height, coordination, activity levels health-related were evaluated in 3 699 Azorean children Portugal, aged 6 10 years. Overweight obesity classified according criteria proposed by Cole et al. year 2000. Logistic regressions used verify associations. Results....