A5052973158- Spectroscopy Techniques in Biomedical and Chemical Research
- Cardiovascular Health and Disease Prevention
- Spectroscopy and Chemometric Analyses
- Non-Invasive Vital Sign Monitoring
- Optical Imaging and Spectroscopy Techniques
- Cutaneous Melanoma Detection and Management
- Semiconductor Lasers and Optical Devices
- Optical Coherence Tomography Applications
- Fern and Epiphyte Biology
- Drug Transport and Resistance Mechanisms
- Molecular Biology Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Blood Pressure and Hypertension Studies
- Fibroblast Growth Factor Research
- AI in cancer detection
- Lichen and fungal ecology
- Plant Pathogens and Fungal Diseases
- Radiation Dose and Imaging
- Plant and animal studies
- Photonic and Optical Devices
- Hemodynamic Monitoring and Therapy
- Sulfur Compounds in Biology
- Analytical Chemistry and Sensors
- Digital Radiography and Breast Imaging
- Inflammatory mediators and NSAID effects
University of Coimbra
2013-2025
Fundação Oswaldo Cruz
2024
Universidade Nova de Lisboa
2019-2022
Erasmus MC
2015-2019
Instituto Português de Oncologia de Coimbra Francisco Gentil
2018-2019
Erasmus MC Cancer Institute
2018
University Medical Center
2017
University Hospital and Clinics
2017
Rotterdam University of Applied Sciences
2017
Escola Superior de Saúde Ribeiro Sanches
2011
Melanoma is a pigmented type of skin cancer, which has the highest mortality all cancers. Because low clinical diagnostic accuracy for melanoma, an objective tool needed to assist assessment lesions that are suspected (early) melanoma. The aim this study was identify spectral differences in CH region HWVN (high-wavenumber) Raman spectra between melanoma and benign melanocytic clinically We used these explore preliminary classification models distinguish from lesions. Data 82 freshly excised...
Clinical diagnosis of early melanoma (Breslow thickness less than 0.8 mm) is crucial to disease-free survival. However, it subjective and can be exceedingly difficult, leading missed melanomas, or unnecessary excision benign pigmented skin lesions. An objective technique needed improve the melanoma. We have developed a method (thin) melanoma, based on Raman spectroscopy. In an ex vivo study in tertiary referral (pigmented lesions) centre, high-wavenumber spectra were collected from 174...
Ovarian cancer is the main cause of death from gynecological cancer, with its poor prognosis mainly related to late diagnosis and chemoresistance (acquired or intrinsic) conventional alkylating reactive oxygen species (ROS)-generating drugs. We others reported that availability cysteine glutathione (GSH) impacts mechanisms resistance carboplatin in ovarian cancer. Different players metabolism can be crucial chemoresistance, such as cystine/glutamate antiporter system Xc (xCT)...
Abstract Species phenology is being altered by ongoing climate changes with yet underappreciated consequences for ecological processes and ecosystem stability. Contrary to what happens some key life events of flowering plants, comparatively little information exists about fern lycophyte how it affected the current climatic changes. In part, this stems from lack long‐term datasets. Here we provide on collection day site spores 121 native introduced (Polypodiopsida) lycopod (Lycopodiopsida)...
(1) Breast cancer is presently the leading cause of death in women worldwide. This study aims at identifying molecular biomarkers human breast cells, order to differentiate highly aggressive triple-negative from non-triple-negative cancers, as well distinct subtypes, which currently an unmet clinical need paramount for improved patient care. (2) Raman and FTIR (Fourier transform infrared) microspectroscopy state-of-the-art techniques were applied, sensitive, specific non-invasive methods...
This study aimed at the development of improved drugs against human osteosarcoma, which is most common primary bone tumor in children and teenagers with a low prognosis. New insights into impact an unconventional Pd(II) anticancer agent on osteosarcoma cells were obtained by synchrotron radiation-Fourier transform infrared microspectroscopy quasi-elastic neutron scattering (QENS) experiments from its effect cellular metabolism to influence intracellular water, can be regarded as potential...
Abstract The biochemical demands associated with tumor proliferation prompt neoplastic cells to augment the import of nutrients sustain their survival and fuel cell growth, a consequent metabolic remodeling. Fatty acids (FA) are crucial in this process, since they have dual role as energetic coins building blocks. Recently, our team has shown that FATP1 pivotal FA transfer between breast cancer (BCCs) non-cancerous microenvironment. We aimed investigate BCCs also explore if inhibition is...
Among gynecologic malignancies, ovarian cancer is the third most prevalent and common cause of death, especially due to diagnosis at an advanced stage together with resistance therapy. As a solid tumor grows, cells in microenvironment are exposed regions hypoxia, selective pressure prompting progression chemoresistance. We have previously shown that cysteine contributes adaptation this hypoxic microenvironment, but mechanisms by which protects from hypoxia-induced death still be unveiled....
Cervical cancer was considered the fourth most common worldwide in 2020. In order to reduce mortality, an early diagnosis of tumor is required. Currently, this type occurs mostly developing countries due lack vaccination and screening against Human Papillomavirus. Thus, there urgent clinical need for new methods aiming at a reliable precancerous cancerous cervical lesions. Vibrational spectroscopy has provided very good results regarding various tumors, particularly using Fourier transform...
With a high potential to generate biomass, maize stover arises as an outstanding feedstock for biofuel production. Maize presents the added advantage of being multiple exploitation crop source food, feed, and energy. In this study, contrasting groups recombinant inbred lines (RILs) from multiparent advanced generation intercross (MAGIC) population that showed variability saccharification efficiency were screened by FTIR-ATR spectroscopy explore compositional differences between low yielders....
Regarding the development of new antineoplastic agents, with a view to assess selective antitumoral potential which aims at causing irreversible damage cancer cells while preserving integrity their healthy counterparts, it is essential evaluate cytotoxic effects in both and malignant human cell lines. In this study, complex two Pd(II) centers linked by biogenic polyamine spermine (Pd2Spm) was tested on (PNT-2) (LNCaP PC-3) prostate lines, using cisplatin as reference. To understand...
Pigmented tissues are inaccessible to Raman spectroscopy using visible laser light because of the high level laser‐induced tissue fluorescence. The fluorescence contribution acquired signal can be reduced by an excitation wavelength in near infrared range around 1000 nm. This will shift spectrum above 1100 nm, which is principal upper detection limit for silicon‐based CCD detectors. For wavelengths nm indium gallium arsenide detectors used. However, InGaAs have not yet demonstrated...
Abstract Raman spectroscopy in the high‐wavenumber spectral region (HWR) is particularly suited for fiber‐optic vivo medical applications. The most‐used materials have negligible signal HWR. This enables use of simple and cheap single‐fiber‐optic probes that can be fitted endoscopes needles. HWR generally shows less tissue luminescence than fingerprint region. However, still stronger signal. Hardware‐ software‐based strategies been developed to correct these signals. Typically,...
Normal-to-malignant transformation is a poorly understood process associated with cellular biomechanical properties. These are strongly dependent on the dynamical behaviour of water, known to play fundamental role in normal activity and maintenance three-dimensional architecture tissue functional state biopolymers. In this study, quasi-elastic neutron scattering was used probe water human cancer specimens their respective surrounding from breast tongue, as an innovative approach for...
Combination of intraoperative wide-field fluorescence imaging and Raman spectroscopy aiding in tumor border identification.
A new type of optical probe based on laser Doppler self-mixing technology, for a truly non-contact measurement in single location, and extraction the temporal features distension wave arterial wall, was developed.The monitoring allows assessment cardiovascular function when is carried out at carotid artery.An algorithm short-time Fourier transform empirical mode decomposition applied to test setup signals determination waveform features, with an accuracy few milliseconds root mean square...
Background: Clinical management guidelines (CMGs) are decision support tools for patient care used by professionals, patients, and family caregivers. Since clinical experts develop numerous CMGs, their technical language hinders comprehension access nonmedical stakeholders. Additionally, the views of affected individuals families often not incorporated into treatment guidelines. We developed an adequate methodology addressing needs preferences professional stakeholders regarding a recently...
Presently the interest in non-invasive devices for monitoring cardiovascular system has increased importance, especially diagnosis of some pathologies. The proposed optical device reveals an attractive instrumental solution local pulse wave velocity (PWV) assessment and other hemodynamic parameters analysis, such as Augmentation Index (AIx), Subendocardial Viability Ratio (SEVR), Maximum Rate Pressure Change (dP/dtmax) Ejection Time (ETI). These allow a better knowledge on condition...