A5049697008- Parasitic infections in humans and animals
- Peroxisome Proliferator-Activated Receptors
- Parasites and Host Interactions
- Polyamine Metabolism and Applications
- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Congenital Anomalies and Fetal Surgery
- Lipid Membrane Structure and Behavior
- Lipid metabolism and biosynthesis
- Coccidia and coccidiosis research
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- Antimicrobial Peptides and Activities
- Parasite Biology and Host Interactions
- Helminth infection and control
- Physiological and biochemical adaptations
- RNA modifications and cancer
- Crystallography and molecular interactions
- Glycosylation and Glycoproteins Research
- thermodynamics and calorimetric analyses
- Enzyme Structure and Function
- Supramolecular Self-Assembly in Materials
- Advanced Proteomics Techniques and Applications
- Enzyme function and inhibition
- Salmonella and Campylobacter epidemiology
Universidad de la República
2007-2023
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2022
Aventura Hospital and Medical Center
2019
Universidade Nova de Lisboa
2014
Universidad de Montevideo
2007-2012
Centro Uruguayo de Imagenología Molecular
2001-2012
Instituto de Investigaciones Biológicas Clemente Estable
2010
Newcastle University
1997
Hospital de Clínicas
1984
Dogs are the main source of human cystic echinococcosis. An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials Morocco and Tunisia a new candidate against Echinococcus granulosus 28 dogs. The was prepared using recombinant proteins from adult worms, tropomyosin (EgTrp) fibrillar protein similar paramyosin (EgA31), cloned expressed live attenuated strain Salmonella enterica serovar typhimurium.In each...
Intracellular lipid binding proteins, including fatty acid proteins (FABPs) 1 and 2, are highly expressed in tissues involved the active metabolism. A zebrafish model was used to demonstrate differential expression levels of fabp1b.1, fabp1b.2, fabp2 transcripts liver, anterior intestine, brain. Transcription fabp1b.1 intestine were upregulated after feeding modulated according diet formulation. Immunofluorescence electron microscopy immunodetection with gold particles localized these FABPs...
Fatty acid-binding proteins (FABPs) are candidate molecules for vaccines against several parasitic platyhelminths. A FABP from the cestode Echinococcus granulosus (EgDf1) was expressed in Salmonella vaccine strains as a C-terminal fusion to fragment C of tetanus toxin (TetC) by using expression vector pTECH. The protein equally attenuated derived bacteria with different genetic backgrounds and attenuating mutations. Single-dose immunization experiments aroA typhimurium strain SL3261 carrying...
Background Growth and maintenance of hydatid cysts produced by Echinococcus granulosus have a high requirement for host lipids biosynthetic processes, membrane building possibly cellular developmental signalling. This requires degree lipid trafficking facilitated transporter proteins. Members the fatty acid binding protein (FABP) family been identified in granulosus, one which, EgFABP1 is expressed at tegumental level protoscoleces, but it has also described both cyst fluid secretions...
TWO MAIN FAMILIES OF LIPID BINDING PROTEINS HAVE BEEN IDENTIFIED IN PARASITIC PLATYHELMINTHES: hydrophobic ligand binding proteins (HLBPs) and fatty acid (FABPs). Members of the former family are specific to Cestoda class, while FABPs conserved across a wide range animal species. Because Platyhelminthes unable synthesize their own lipids, these lipid-binding important molecules in organisms. HLBPs high molecular mass complex lipids. They composed subunits low array lipid ranging from CoA...
This work describes two new fatty acid binding proteins (FABPs) identified in the parasite platyhelminth Mesocestoides vogae (syn. corti). The corresponding polypeptide chains share 62% identical residues and overall 90% similarity according to CLUSTALX default conditions. Compared with Cestoda FABPs, these highest score Taenia solium protein. M. FABPs are also phylogenetically related FABP3/FABP4 mammalian FABP subfamilies. native were purified by chromatographical procedures, apparent...
Abstract The in vivo uptake by neuroepithelial cells of rat serum proteins injected into the mesencephalic cavity developing chicken was demonstrated immunohistochemical methods. It found that 48 h after inoculation, alphafetoprotein (AFP) and albumin (SA) localize same places as do AFP SA. No labelling present for or IgG. We also evidence showing ovalbumin, which is not normally inside ependymal internalized even when cerebrospinal fluid at 30‐fold its physiological concentration. existence...
The protein ReP1-NCXSQ was isolated from the cytosol of squid nerves and has been shown to be required for MgATP stimulation nerve Na+/Ca2+ exchanger NCXSQ1. In order determine its mode action corresponding biologically active ligand, sequence analysis, crystal structures mass-spectrometric studies this Tyr128Phe mutant are reported. Sequence analysis suggests that it belongs CRABP family in FABP superfamily. X-ray structure at 1.28 Å resolution shows β-barrel fold, with a fatty acid inside...
Nuclear receptors are ligand-activated transcription factors capable of regulating the expression complex gene networks. The family includes seven subfamilies proteins with a wide phylogenetic distribution. A novel subfamily two DNA-binding domains (2DBDs) has been reported in Schistosoma mansoni (Platyhelminth, Trematoda). This work describes cDNA cloning and bioinformatics analysis Eg2DBDα, 2DBD nuclear receptor isoform from parasite Echinococcus granulosus Cestoda). Eg2DBDα coding domain...
Nuclear receptors are ligand-activated transcription factors capable of regulating the expression complex gene networks. The family includes seven subfamilies protein with a wide phylogenetic distribution. A novel subfamily two DNA-binding domains (2DBDs) has been first reported in Schistosoma mansoni (Platyhelminth, Trematoda). Employing an ab initio protocol and homology modeling methods, full-length 3D structure Eg2DBDα.1 nuclear receptor from Echinococcus granulosus Cestoda) was...
Fatty acid (FA) binding proteins are small intracellular whose members exhibit great diversity and low similarity at the primary structure level, but a highly conserved three-dimensional structure. Characterised by high-affinity non-covalent of hydrophobic ligands, these have molecular mass 14-15 kDa with characteristic β-barrel Members this family been identified along zoological scale, Platyhelminthes being more primitive organisms where they reported. Two FA (FABPs), EgFABP1 EgFABP2, 88%...
SUMMARY Successful host invasion by parasitic helminths involves detection and appropriate response to a range of host-derived signals. Insulin signal pathways are ancient highly-conserved throughout the metazoans. However, very little is known about helminth insulin signalling potential role it may play in host-parasite interactions. The Mesocestoides vogae (Cestoda: Cyclophyllidea) larvae human was investigated, focusing on tyrosine-phosphorylation status, glucose content, survival asexual...
Two fatty acid binding proteins, MvFABPa and MvFABPb were identified in the parasite Mesocestoides vogae (Platyhelmithes, Cestoda). Fatty proteins are small intracellular whose members exhibit great diversity. Proteins of this family have been many organisms, which Platyhelminthes among most primitive. These particular relevance flatworms since de novo synthesis acids is absent. should be captured from media needing an efficient transport system to uptake distribute these molecules. While...