A5030778973- Hereditary Neurological Disorders
- Nerve injury and regeneration
- Toxin Mechanisms and Immunotoxins
- Calcium signaling and nucleotide metabolism
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Skin and Cellular Biology Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Endoplasmic Reticulum Stress and Disease
- Cellular Mechanics and Interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Optical Imaging and Spectroscopy Techniques
- Parasitic infections in humans and animals
- Neurogenesis and neuroplasticity mechanisms
- Parasites and Host Interactions
- Curcumin's Biomedical Applications
- Mitochondrial Function and Pathology
- Peripheral Neuropathies and Disorders
- melanin and skin pigmentation
- Synthesis and Characterization of Heterocyclic Compounds
- Microtubule and mitosis dynamics
- Signaling Pathways in Disease
- PARP inhibition in cancer therapy
- RNA regulation and disease
Universidad de la República de Uruguay
2013-2024
Instituto de Investigaciones Biológicas Clemente Estable
2013-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2024
University of Virginia
2013
Comisión Sectorial de Investigación Científica
2012
Ministerio de Educación y Cultura
2007
ENVIRON (United States)
1997
Universidad La República
1997
San Francisco State University
1997
There is an urgent need for the research of close and enigmatic relationship between nerve biomechanics development neuropathies. Here we present a strategy based on application atomic force confocal microscopy simultaneous integrity investigations. Using wild-type hereditary neuropathy mouse models, reveal surprising mechanical protection peripheral nerves. Myelinated fibres promptly fully recover from acute enormous local compression while maintaining functional structural integrity. The...
Local protein synthesis within axons has been studied on a limited scale. In the present study, several techniques were used to investigate this in sciatic nerve, and show that it increases after damage axon. Neurofilament (NF) mRNAs probed by RT-PCR, Northern blot situ hybridization of intact rat proximal or distal stumps nerve transection. RT-PCR demonstrated presence NF-L, NF-M NF-H as well severed nerves. analysis detected NF-L NF-M, but not NF-H. This technique did detect three NFs...
The distribution of ribosomes and polysomes in uninjured myelinated axons rat sciatic nerve was analyzed. Ribosomes were identified by immunocytochemistry at the light electron microscopic levels. A polyclonal antibody developed against recognized both rRNA ribosomal proteins. immunoreaction product similar to that obtained with human anti-ribosomal P protein. distributions two types axons: 1) periodic localization cortical region axoplasm appeared as a compact structural aggregate,...
In free-living flatworms somatic differentiated cells do not divide, and a separate population of stem (called neoblasts) is responsible for cell proliferation renewal. cestodes, there evidence that similar mechanisms renewal exist.In this work, we have characterized proliferative during the development model cestode Mesocestoides corti from larva (tetrathyridium) to young segmented worm. This was done by two complementary strategies with congruent results: characterizing in S phase their...
To better understand the role of protein synthesis in axons, we have identified source a portion axonal RNA. We show that proximal segments transected sciatic nerves accumulate newly-synthesized RNA axons. This is synthesized Schwann cells because was labeled complete absence neuronal cell bodies both vitro and vivo. also demonstrate transfer prevented by disruption actin it fails to occur myosin-Va. Our results cell-to-cell identify part mechanism required for transfer. The induction injury...
Curcumin is a polyphenol extracted from Curcuma longa's roots. Low doses of curcumin are related to anti-inflammatory, antioxidant, and neuroprotective effects, while high used for their lethality. This diversity behaviors allows us understand as compound with hormetic action. Due its strongly hydrophobic character, often solubilized in organic compounds. In this way, we have recently reported the undesirable occasionally irreversible effects alcohol DMSO on viability primary Schwann cell...
Abstract Very little is known about the function of F‐actin cytoskeleton in regeneration and pathology peripheral nerve fibers. The actin has been associated with maintenance tissue structure, transmission traction contraction forces, an involvement cell motility. Therefore, state strongly influences mechanical properties cells intracellular transport therein. In this work, we analyze distribution at Schmidt‐Lanterman Incisures (SLI) nodes Ranvier (NR) domains normal, regenerating pathologic...
Myelination of the peripheral nervous system requires Schwann cells (SC) differentiation into myelinating phenotype. The myelin protein-22 (PMP22) is an integral membrane glycoprotein, expressed in SC. It was initially described as a growth arrest-specific (gas3) gene product, up-regulated by serum starvation. PMP22 mutations were pathognomonic for human hereditary neuropathies, including Charcot-Marie-Tooth disease (CMT). Trembler-J (TrJ) heterozygous mouse model carrying same pmp22 point...
The accumulation of misfolded proteins is associated with various neurodegenerative conditions. Mutations in PMP‐22 are the human peripheral neuropathy, Charcot–Marie–Tooth Type 1A (CMT1A). a short‐lived 22 kDa glycoprotein, which plays key role maintenance myelin structure and compaction, highly expressed by Schwann cells. It forms aggregates when proteasome inhibited or protein mutated. This study reports application atomic force microscopy (AFM) as detector profound topographical...
Poly-ADP-ribose (PAR) is a polymer of up to 400 ADP-ribose units synthesized by poly-ADP-ribose-polymerases (PARPs) and degraded poly-ADP-ribose-glycohydrolase (PARG). Nuclear PAR modulates chromatin compaction, affecting nuclear functions (gene expression, DNA repair). Diverse defined PARP cytoplasmic allocation patterns contrast with the yet still imprecise distribution unclear functions. Based on previous evidence from other models, we hypothesized that could be present in epithelial...
Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal regenerating peripheral nerves. Previously, we have shown that transfer is dependent on F-actin cytoskeleton Myosin Va. Here, explored the contribution microtubules to newly synthesized transport cell nuclei up nodal microvilli sciatic Results using immunohistochemistry quantitative confocal FRET analysis indicate cell-derived co-localize with cytoplasm. Additionally, nocodazole colchicine sensitive...
Charcot-Marie-Tooth (CMT) type 1 disease is the most common human hereditary demyelinating neuropathy. Mutations in pmp22 cause about 70% of all CMT1. Trembler-J (TrJ/+) mice are an animal model CMT1E, having same spontaneous mutation that found humans. We compared behavior profile TrJ/+ and +/+ (wild-type) open-field elevated-plus-maze anxiety tests. In these tests, showed exclusive head shake movement, a lower frequency rearing, but greater grooming. elevated-plus-maze, defecate more...
Abstract The presence of Myosin Va (an actin‐based molecular motor) in the peripheral nervous system was examined and its subcellular distribution within axons sciatic nerve demonstrated via immunocytochemistry. (M‐Va) detected by using SDS‐PAGE Western blot techniques with a polyclonal antibody specifically raised against M‐Va globular tail domain. In addition, purification from rat prior to immunoblotting yielded standard band. Likewise, optical immunocytochemical procedures revealed M‐Va,...
Charcot-Marie-Tooth (CMT) syndrome is the most common progressive human motor and sensory peripheral neuropathy. CMT type 1E a demyelinating neuropathy affecting Schwann cells due to peripheral-myelin-protein-22 (PMP22) mutations, modelized by Trembler-J mice. Curcumin, natural polyphenol compound obtained from turmeric (Curcuma longa), exhibits dose- time-varying antitumor, antioxidant neuroprotective properties, however, neurotherapeutic actions of curcumin remain elusive. Here, we propose...