A5088742599- Malaria Research and Control
- Neonatal Health and Biochemistry
- Aquaculture Nutrition and Growth
- Mosquito-borne diseases and control
- Aquaculture disease management and microbiota
- Identification and Quantification in Food
- Reproductive biology and impacts on aquatic species
- Rabbits: Nutrition, Reproduction, Health
- Erythrocyte Function and Pathophysiology
- Blood disorders and treatments
- RNA and protein synthesis mechanisms
- Genetic diversity and population structure
- Peroxisome Proliferator-Activated Receptors
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Meat and Animal Product Quality
- Sperm and Testicular Function
- Enzyme Catalysis and Immobilization
- Methemoglobinemia and Tumor Lysis Syndrome
- vaccines and immunoinformatics approaches
- Vector-borne infectious diseases
- Metabolism and Genetic Disorders
- Animal Nutrition and Physiology
- Ichthyology and Marine Biology
- Complement system in diseases
Universidad Complutense de Madrid
2016-2025
Research Institute Hospital 12 de Octubre
2014-2024
Hospital Universitario 12 De Octubre
2017-2018
Universidad Continental
2017
Earth and Space Research
2013-2014
University of Washington
2013-2014
National Agricultural Research Foundation
2010
ANFACO-CECOPESCA
2010
Cabildo de Tenerife
2007
Universidad de La Laguna
2007
Illegal, Unreported and Unregulated fishing has had a major role in the overexploitation of global fish populations. In response, international regulations have been imposed many fisheries 'eco-certified' by consumer organizations, but methods for independent control catch certificates eco-labels are urgently needed. Here we show that, using gene-associated single nucleotide polymorphisms, individual marine can be assigned back to population origin with unprecedented high levels precision....
Lipids are the predominant source of energy for fish. The mechanisms by which fish allocate from lipids metabolism, development, growth, and reproduction critical understanding key life-history strategies transitions. Currently, major lipid component in aquaculture diets is oil (FO), derived wild capture fisheries that exploited at their maximum sustainable limit. increasing demand FO will soon exceed supply threaten viability aquaculture. Thus, it essential to minimize use diets. This might...
Abstract This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according their mitochondrial cytochrome b nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine comprising the main Actinopterygii family groups. When used phylogenetic analyses, its combination two genes with different evolutionary rates serves identify at level. We provide flow diagram...
Linseed (LO) and soyabean (SO) oils were evaluated as fish-oil (FO) substitutes in the diets of marketable-sized gilthead sea bream ( Sparus aurata ). Practical designed factorially with lipid added follows (%): FO 100, LO 60+FO 40, 80+FO 20, SO 20. The effects experimental on growth, fatty acids patterns liver muscle, flesh quality variables activities selected enzymes involved synthesis catabolism determined at end a 7-month trial. Fatty acid composition muscle generally reflected diets. n...
The cloning and characterization of cDNAs genes encoding three peroxisome proliferator-activated receptor (PPAR) isotypes from two species marine fish, the plaice (Pleuronectes platessa) gilthead sea bream (Sparus aurata), are reported for first time. Although differences in genomic organization fish PPAR compared with their mammalian counterparts evident, sequence alignments phylogenetic comparisons show to be homologs PPARalpha, PPARbeta/delta, PPARgamma. Like homologs, PPARs bind a...
Mouse embryonic stem (ES) glucose-6-phosphate (G6P) dehydrogenase-deleted cells ( G6pd delta), obtained by transient Cre recombinase expression in a -loxed cell line, are unable to produce G6P dehydrogenase (G6PD) protein (EC 1.1.1.42). These delta proliferate vitro without special requirements but extremely sensitive oxidative stress. Under normal growth conditions, ES show high ratio of NADPH NADP(+) and intracellular level GSH. In the presence thiol scavenger oxidant, azodicarboxylic acid...
Rainbow trout (Oncorhynchus mykiss) hepatocytes were cultured under simulated conditions of varying nutritional status to explore the short-term modulation by dietary substrates main lipogenic enzymes: glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME), ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACoAC) and fatty acid synthetase (FAS). Primary cultures individually exposed amounts glucose, hydrolysed casein long-chain polyunsaturated acids (PUFA) for 12 h. A second set...
Significance Malaria remains one of the main health threats in developing world, with staggering social and economic costs. Resistance to artemisins, pharmacological tool currently available against malaria, has been widely reported. Borrelidin, a natural compound that inhibits threonyl-tRNA synthetase, long studied for its antibacterial antiparasitic properties, but undesirable toxic effects prevented further clinical development. Here we present group borrelidin derivatives retain their...
The objective of this experiment was to test the effect total or partial substitution dietary fish oil (FO) by linseed (LO) in Atlantic salmon feeding on performance, liver and muscle fatty acid composition, selected lipogenic lipolytic enzyme activities, flesh oxidative stability. For 12 wk, (220 ± g initial BW) were fed five experimental diets which FO serially replaced 25, 50, 75, 100% LO. Total replacement LO did not (P = 0.20) affect final weight, biometric indices, i.m. fat contents....
Nna1 has some sequence similarity to metallocarboxypeptidases, but the biochemical characterization of not previously been reported. In this work we performed a detailed genomic scan and found >100 homologues in bacteria, Protista, Animalia, including several paralogs most eukaryotic species. Phylogenetic analysis Nna1-like sequences demonstrates major divergence between peptidases known metallocarboxypeptidases subfamilies: M14A, M14B, M14C. Conformational modeling representative proteins...
Mitochondrial 16S rRNA sequences from morphological validated grouper (Epinephelus aeneus, E. caninus, costae, and marginatus; Mycteroperca fusca M. rubra), Nile perch (Lates niloticus), wreck fish (Polyprion americanus) were used to develop an analytical system for group diagnosis based on two alternative Polymerase Chain Reaction (PCR) approaches. The first includes conventional multiplex PCR in which electrophoretic migration of different sizes bands allowed identification the species....