A5012153269- Liver Disease Diagnosis and Treatment
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Aquaculture Nutrition and Growth
- Liver Disease and Transplantation
- Viral Infections and Immunology Research
- Liver physiology and pathology
- Animal Virus Infections Studies
- Drug-Induced Hepatotoxicity and Protection
- Circadian rhythm and melatonin
- Aquaculture disease management and microbiota
- Hepatitis B Virus Studies
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- Animal Nutrition and Physiology
- Energy and Environment Impacts
- Enzyme Production and Characterization
- Welding Techniques and Residual Stresses
- Trypanosoma species research and implications
- Hydrogen embrittlement and corrosion behaviors in metals
- Sphingolipid Metabolism and Signaling
- Reproductive biology and impacts on aquatic species
- Hepatitis Viruses Studies and Epidemiology
- Autophagy in Disease and Therapy
Hospital Universitario del Tajo
2025
Hospital Universitario Príncipe de Asturias
2006-2024
Universidad de León
2009-2020
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2009
Universidad de Navarra
2009
Fremantle Hospital
2009
The University of Western Australia
2009
Centro de Investigacion Principe Felipe
2009
Cornell University
2009
Hospital de León
2003-2009
Summary Faeces samples from diarrhoeic and non-diarrhoeic lambs goat kids aged 1–45 days were examined for enteric pathogens. Cryptosporidium parvum was detected in both (45%) (42%) but not animals. F5 + (K99 ) and/or F41 Escherichia coli strains isolated 26% 22% of the kids, respectively, although these strains, which did produce enterotoxins ST I or LT I, found with similar frequencies A − E. strain a lamb (0·6%). Verotoxigenic (4·1% 8·2%, respectively) there no association between...
Rainbow trout (Oncorhynchus mykiss) hepatocytes were cultured under simulated conditions of varying nutritional status to explore the short-term modulation by dietary substrates main lipogenic enzymes: glucose-6-phosphate dehydrogenase (G6PD), malic enzyme (ME), ATP-citrate lyase (ACL), acetyl-CoA carboxylase (ACoAC) and fatty acid synthetase (FAS). Primary cultures individually exposed amounts glucose, hydrolysed casein long-chain polyunsaturated acids (PUFA) for 12 h. A second set...
The antiviral activity versus flaviviruses of artemisinin, a safe drug obtained from Artemisia annua and commonly used to treat malaria, has been investigated using as an IN VITRO model bovine epithelial cells embryonic trachea (EBTr) infected with the cytopathic strain Oregon C24V, viral diarrhoea virus (BVDV), which is member Flaviviridae family. Antiviral was estimated by degree protection against effect BVDV on host reduction in BVDV-RNA release culture medium. To induce intermediate...
Abstract: The objective of the present study was to investigate effect melatonin on liver inflammatory and regenerative response in an animal model fulminant hepatic failure (FHF) viral origin. Rabbits were experimentally infected with 2 × 10 4 hemagglutination units a rabbit hemorrhagic disease virus (RHDV) isolate received at two concentrations or 20 mg/kg 0, 12 24 hr postinfection. RHDV infection induced response, increased expression toll‐like receptor 4, high‐mobility group box (HMGB)1,...
Disruption of circadian rhythms, which are regulated by the clock machinery, plays an important role in different long-term diseases including hepatocellular carcinoma (HCC). Melatonin has been reported to alleviate promotion and progression HCC, but potential contribution modulation is unknown. We investigated effects melatonin mice received diethylnitrosamine (DEN) (35 mg/kg body weight ip) once a week for 8 weeks. was given at 5 or 10 mg kg
Hepatocyte apoptosis plays an important role in the development of fulminant hepatic failure (FHF). The objective this study was to investigate antiapoptotic effect melatonin animal model FHF viral origin induced by rabbit hemorrhagic disease virus (RHDV). Rabbits were experimentally infected with 2 × 10(4) hemagglutination units a RHDV isolate and received at two concentrations 10 20 mg/kg 0, 12, 24 hr postinfection. infection liver apoptosis, increased caspase-3 immunoexpression activity...
Abstract: This work was undertaken to investigate whether treatment with melatonin prevents oxidative stress and changes in the expression activity of factor erythroid 2-related 2 (Nrf2)-mediated antioxidant enzymes an animal model fulminant hepatic failure viral origin. Rabbits were experimentally infected × 104 hemagglutination units a rabbit hemorrhagic disease virus (RHDV) isolate received at two concentrations 10 mg/kg 20 0, 12 24 hr postinfection. Blood transaminases, blood lactate...
Abstract Hepatocyte apoptosis plays an important role in the development of fulminant hepatic failure ( FHF ). The objective this study was to investigate whether endoplasmic reticulum ER ) stress and unfolded protein response UPR inhibition is underlying mechanism melatonin anti‐apoptotic effects animal model viral origin induced by rabbit hemorrhagic disease virus RHDV Rabbits were experimentally infected with 2 × 10 4 hemagglutination units a isolate received at two concentrations mg/kg...
Autophagy is an important survival pathway and participates in the host response to infection. Beneficial effects of melatonin have been previously reported animal model acute liver failure (ALF) induced by rabbit hemorrhagic disease virus (RHDV). This study was aimed investigate whether protection against injury RHDV associates modulation autophagy. Rabbits were infected with 2 × 10(4) hemagglutination units a isolate received 20 mg/kg at 0, 12, 24 hr postinfection. autophagy, increased...
Abstract The sphingosine kinase 1/sphingosine 1‐phosphate (SphK1/S1P) system is involved in different pathological processes, including fibrogenesis. Melatonin abrogates activation of hepatic stellate cells (HSCs) and attenuates profibrogenic pathways animal models fibrosis, but it unknown if protection associates with its inhibitory effect on the SphK1/S1P axis. Mice treatment groups received carbon tetrachloride (CCl 4 ) 5 μL g −1 body wt i.p. twice a week for or 6 weeks. was given at 10...
The effects of dietary fish oil and digestible protein (DP) levels on muscle fatty acid composition susceptibility to lipid peroxidation were studied in two representative species for human nutrition, from fresh seawater, rainbow trout (Oncorhynchus mykiss) European sea bass (Dicentrarchus labrax). In trout, higher concentrations fat DP led weight gain (g/d) (P = 0.001 P 0.043 respectively). Additionally, an interaction effect was observed this species, since the only evident when...
Abstract The haemorrhagic disease virus (RHDV) is a non‐cultivable that promotes in rabbits an acute which accomplishes many characteristics of animal model fulminant hepatic failure (FHF). Beneficial effects melatonin have been reported RHDV‐infected rabbits. This study investigated whether protection against viral‐derived liver injury by associated with modulation mitophagy, innate immunity and clock signalling. Rabbits were experimentally infected 2 × 10 4 haemagglutination units RHDV...
In a previous study, we found fecal colonization with multiresistant Escherichia coli exhibiting high-level trimethoprim resistance in 19% of diapered children attending six day-care centers Houston, Tex. To examine the potential risk factors associated this finding, conducted cross-sectional studies among 203 12 centers, 51 well-child clinic (controls), and 64 medical students. The prevalence trimethoprim-resistant E. (30%) was higher (P less than 0.001) control (6%) or students (8%)....
ABSTRACT Rabbit hemorrhagic disease virus (RHDV) causes lethal fulminant hepatitis closely resembling acute liver failure (ALF) in humans. In this study, we investigated whether cardiotrophin-1 (CT-1), a cytokine with hepatoprotective properties, could attenuate damage and prolong survival virus-induced ALF. Twenty-four rabbits were infected 2 × 10 4 hemagglutination units of RHDV. Twelve received five doses CT-1 (100 μg/kg) starting at 12 h postinfection (hpi) (the first three every 6 then...
Abstract The sphingosine kinase (SphK)1/sphingosine‐1‐phosphate (S1P) pathway is involved in multiple biological processes, including liver diseases. This study investigate whether modulation of the SphK1/S1P system associates to beneficial effects melatonin an animal model acute failure ( ALF ) induced by rabbit hemorrhagic disease virus RHDV ). Rabbits were experimentally infected with 2 × 10 4 hemagglutination units a isolate and received 20 mg/kg at 0, 12, 24 hr postinfection. Liver mRNA...
Management of fulminant hepatic failure (FHF) continues to be one challenging problem, and experimental animal models resembling its clinical conditions are still needed.Rabbit hemorrhagic disease (RHD) fullfils many requirements an model FHF.This work investigated changes in MAPK, NF-jB, AP-1 STAT pathways during RHD-induced liver injury.Rabbits were infected with 2 • 10 4 hemagglutination units RHD virus isolate.Apoptosis was documented by the presence caspase-3 activity substantial PARP...