The effects of altered thyroid states on lipid peroxidation, antioxidant capacity, and susceptibility to oxidative stress rat tissues were examined. Hypothyroidism was induced by administering methimazole in drinking water for 15 days. Hyperthyroidism elicited a 10-day treatment hypothyroid rats with tri-iodothyronine (10 micrograms/100 g body weight). In the peroxidation not modified, whereas hyperthyroid increased liver heart but skeletal muscle. glutathione peroxidase activity...

Mouse embryonic stem (ES) glucose-6-phosphate (G6P) dehydrogenase-deleted cells ( G6pd delta), obtained by transient Cre recombinase expression in a -loxed cell line, are unable to produce G6P dehydrogenase (G6PD) protein (EC 1.1.1.42). These delta proliferate vitro without special requirements but extremely sensitive oxidative stress. Under normal growth conditions, ES show high ratio of NADPH NADP(+) and intracellular level GSH. In the presence thiol scavenger oxidant, azodicarboxylic acid...

Fresh products are characterized by reduced shelf-life because they an excellent growth medium for a lot of microorganisms. Therefore, the microbial spoilage causing significant food supply losses has become enormous economic and ethical problem worldwide. The antimicrobial packaging is offering viable solution to tackle this safety issue extending improving quality fresh products. goal study was investigate effects contact surface polyethylene terephthalate (PET) functionalized with...

Antimicrobial activity of many AMPs can be improved by lysine-to-arginine substitution due to a more favourable interaction arginine guanidinium moiety with bacterial membranes. In previous work, the structural and functional characterization an amphipathic antimicrobial peptide named RiLK1, including lysine as positively charged amino acids in its sequence, was reported. Specifically, RiLK1 retained β-sheet structure under wide range environmental conditions (temperature, pH, ionic...

Cataract is a prevalent health condition, primarily caused by aging, affecting approximately 95 million individuals worldwide. The only effective treatment currently involves surgically removing and replacing the crystalline lens with an artificial intraocular (IOL). Various IOLs are available, each distinct characteristics, costs, outcomes. This study aimed to assess value of enhanced monofocal IOL for cataract surgery, which has been shown improve intermediate vision reduce need spectacles...

The Arctic and Antarctic marine faunas differ by age isolation. Fishes of the two polar regions have undergone different regional histories that driven physiological diversities. fish are highly stenothermal, in keeping with stable water temperatures, whereas fish, being exposed to seasonal temperature variations, exhibit higher plasticity. This study reports characterization oxygen transport system three species family Gadidae, namely cod Arctogadus glacialis, Boreogadus saida, Atlantic...

Cell surface proteins of hyperthermophilic Archaea actively participate in intercellular communication, cellular uptake, and energy conversion to sustain survival strategies extreme habitats. Surface (S)-layer glycoproteins, the major component S-layers many archaeal species best-characterized prokaryotic were shown have a large structural diversity their glycan compositions. In spite this, knowledge on glycosylation other than S-layer is quite limited. Here, N-glycosylation pattern...

Synthetic antibacterial peptides are advanced weapons that scientists design and produce to confront current threats of harmful mortal pathogens, which could affect humans in everyday life. Recently, many small amino acid sequences, greatly efficient their action, have been reported the literature. To date, only a few synthetic peptides, acting at micromolar or even tenths concentrations, on market as commercial products, mainly because high cost production. In this context, materials...

Food packaging is not only a simple protective barrier, but real “active” component, which expected to preserve food quality, safety and shelf-life. Therefore, the materials used for production should show peculiar features properties. Specifically, antimicrobial has recently gained great attention with respect both social economic impacts. In this paper, results obtained by using polymer material functionalized small synthetic peptide as are reported. The surface of Polyethylene...

Fresh fish are highly perishable, owing mainly to their moisture content, high amount of free amino acids and polyunsaturated fatty acids. Microorganisms chemical reactions cause the spoilage, leading loss in quality, human health risks a market value reduction. Therefore, fishing industry has always been willing explore new technologies increase quality safety products through decrease microbiological biochemical damage. In this context, antimicrobial active packaging is one such promising...

A sensitive and reproducible HPLC assay with fluorometric detection was used to measure the malondialdehyde (MDA) concentration in food (butter, margarine, oil, fish, meat tissue). Samples were homogenized water supplemented butylated hydroxytoluene. Proteins precipitated ice-cold 5% trichloroacetic acid removed by centrifugation. The supernatant incubated a 0.28% thiobarbituric (TBA) mixture from which oxygen depleted. Optimal incubation time temperature, for TBA treatment, found be 30 min...

Abstract Scope : The involvement of oxidative stress in gluten‐induced toxicity has been evidenced vitro and clinical studies but never examined vivo. We recently demonstrated the protective activity conjugated linoleic acid (CLA), which functions by activation nuclear factor erythroid 2‐related factor2 (Nrf2), a key transcription for synthesis antioxidant detoxifying enzymes (phase 2). Here, we evaluate gliadin‐mediated human Caco‐2 intestinal cells gliadin‐sensitive leukocyte antigen‐DQ8...

Abstract The activity of the enzyme lecithin‐cholesterol acyltransferase (LCAT; E.C. 2.3.1.43) is involved in removal cholesterol excess from peripheral cells. This stimulated by HDL (high density lipoprotein) apolipoprotein A1 (ApoA1). Haptoglobin (Hpt) was previously found to be associated with ApoA1 ovarian follicular fluid. LCAT analyzed fluids, collected an IVF program, containing different amounts Hpt or Hpt/ApoA1 ratio. Addition purified fluid caused a decrease activity, which...

Oxidative damages to the oocyte or follicular cells were suggested trigger atresia. In water buffalo, loss of blood-follicle barrier sieving effect on diffusion plasma haptoglobin was previously found be associated with atretic oocytes. The redox status buffalo follicles evaluated by measuring in fluid both total antioxidant capacity (TAC), expressed as Trolox equivalents, and concentration specific free radical scavengers, determined high-performance liquid chromatography. Among at random...

Controlled ovarian stimulation was induced in 19 women for vitro fertilisation/embryo transfer. After ovum pick-up, haptoglobin titres were determined by ELISA sera and homologous follicular fluids. The phenotype of each subject assessed the penetration protein forms through blood-follicle barrier predicted on basis their molecular weight. threshold compatible with integrity calculated as 92%, 73% 57% blood level phenotypes Hpt 1-1, 1-2 2-2 respectively. Penetration values comparable/lower...

SSO1273 of Sulfolobus solfataricus was identified as a cell surface-bound protein by proteomics approach. Sequence inspection the genome revealed that open reading frame sso1273 is associated in an operon-like structure with genes encoding all remaining components canonical protein-dependent ATP-binding cassette (ABC) transporter. gene expression and accumulation on surface were demonstrated to be strongly induced addition peptide mixture (tryptone) culture medium. The native obtained...

<b><i>Purpose:</i></b> To evaluate the efficacy of one intravitreal injection dexamethasone (Ozurdex®; Allergan, Inc., Irvine, Calif., USA) in serous macular detachment (SMD) eye, associated with bilateral central retinal vein occlusion (CRVO) a patient affected by Waldenström's macroglobulinemia (WM). <b><i>Patients and Methods:</i></b> A female patient, WM, complained progressive decrease visual acuity, mainly left eye (LE). SMD LE CRVO was...

Oxidative challenge is an important factor affecting the adaptive strategies of Antarctic fish, but data on antioxidant defenses in these organisms remain scarce. In this context, a key role could be played by acylpeptide hydrolase (APEH), which was recently hypothesized to participate degradation oxidized and cytotoxic proteins, although its physiological function still not fully clarified. This study represents first report piscine members enzyme family, specifically from teleost...

The proteasome is a multienzymatic complex that controls the half-life of majority intracellular proteins, including those involved in apoptosis and cell-cycle progression. Recently, inhibition has been shown to be an effective anticancer strategy, although its downregulation often accompanied by severe undesired side effects. We previously reported acylpeptide hydrolase (APEH) peptide SsCEI 4 can significantly affect activity A375 melanoma or Caco-2 adenocarcinoma cell lines, thus shedding...

Alzheimer's disease (AD) is a progressive, multifactorial neurodegenerative disorder that the main cause of dementia. To date, there are no definitive diagnostic tests can predict or assess onset and progression disease. Blood biomarkers for AD being sought many years but ir identification remains challenging task. In this study, we investigated potential relationship between levels acyl-peptide hydrolase (APEH) proteasome in erythrocyte samples 52 participants (26 26 cognitively healthy...

Aims To evaluate deep corneal densitometry and visual outcomes after big-bubble (BB-DALK) visco-bubble (VB-DALK) anterior lamellar keratoplasty performed in patients with keratoconus. Methods Prospective comparative study of 50 advanced keratoconic who underwent DALK surgery; 25 eyes (group I) were completed BB-DALK II) VB-DALK the failure pneumatic dissection. Best spectacle-corrected acuity (BSCVA), tomographic parameters endothelial cell count recorded 1, 3, 6, 12 24 months surgery....