A5074325380- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Glycosylation and Glycoproteins Research
- Advanced Fiber Optic Sensors
- Peptidase Inhibition and Analysis
- Photonic and Optical Devices
- Cell death mechanisms and regulation
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- Protein purification and stability
- Click Chemistry and Applications
- Ubiquitin and proteasome pathways
- Melanoma and MAPK Pathways
- Protein Kinase Regulation and GTPase Signaling
- Angiogenesis and VEGF in Cancer
- Alzheimer's disease research and treatments
- Hepatitis C virus research
- Advanced Biosensing Techniques and Applications
- NF-κB Signaling Pathways
- Protein Structure and Dynamics
- Plasmonic and Surface Plasmon Research
- RNA Interference and Gene Delivery
- Prion Diseases and Protein Misfolding
- RNA Research and Splicing
- Mast cells and histamine
Institute of Biostructure and Bioimaging
2016-2025
National Research Council
2015-2024
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi
2009-2019
University of Naples Federico II
2007-2018
Naples Anesthesia & Physician Associates
2016
National Academies of Sciences, Engineering, and Medicine
2010-2015
National Research Council
2015
Imperial College London
2014
University of Oxford
2011
Nara Institute of Science and Technology
2011
IL-36 cytokines, a subgroup of IL-1 family, comprise IL-36α, IL-36β, and IL-36γ agonists, abundantly expressed in psoriatic skin, IL-36RA IL-38 antagonists. In cytokines interfere with keratinocyte cornification programs induce the release antimicrobial peptides chemokines active on neutrophils Th17 lymphocytes. To date, role antagonist psoriasis remains to be defined. Here, we demonstrate that skin circulating levels are reduced patients other diseases characterized by neutrophilic...
Myocyte death occurs in many inherited and acquired cardiomyopathies, including arrhythmogenic cardiomyopathy (ACM), a genetic heart disease plagued by the prevalence of sudden cardiac death. Individuals with ACM harboring pathogenic desmosomal variants, such as desmoglein-2 (DSG2), often show myocyte necrosis progression to exercise-associated failure. Here, we showed that homozygous Dsg2 mutant mice (Dsg2 mut/mut), model ACM, die prematurely during swimming display myocardial dysfunction...
A peptide mimicking protein for its ability to recognize the Fc immunoglobulin portion has been identified through screening of a synthetic multimeric library. Screening library, composed randomized tripeptide tetramers, carried out using very simple assay, measuring library interfere with interaction between and biotinylated immunoglobulins, monitored on solid phase an enzyme-linked immunosorbent assay format. The tetrameric after three cycles was produced in larger amounts then immobilized...
IL-32 is a multifaceted cytokine with role in infections, autoimmune diseases, and cancer, it exerts diverse functions, including aggravation of inflammation inhibition virus propagation. We previously identified as critical regulator endothelial cell (EC) we now reveal that also possesses angiogenic properties. The hyperproliferative ECs human pulmonary arterial hypertension glioblastoma multiforme exhibited markedly increased abundance IL-32, and, significantly, the colocalized integrin...
In this paper we report the synthesis of a series benzoxaborole derivatives, their inhibition properties against some carbonic anhydrases (CAs), recognized as important drug targets, and characterization binding mode these molecules to CA active site. Our data provide first experimental evidence that benzoxaboroles can be efficiently used inhibitors.
Background The host’s immune system develops in equilibrium with both cellular self-antigens and non-self-antigens derived from microorganisms which enter the body during lifetime. In addition, years, a tumor may arise presenting to an additional pool of non-self-antigens, namely antigens (tumor-associated antigens, TAAs; tumor-specific TSAs). Methods present study, we looked for homology between published TAAs non-self-viral-derived epitopes. Bioinformatics analyses ex vivo immunological...
Introduction: Currently, conventional treatments of hepatocellular carcinoma (HCC) are not selective enough for tumor tissue and lead to multidrug resistance drug toxicity. Although sorafenib (SOR) is the standard first-line systemic therapy approved clinical treatment HCC, its poor aqueous solubility rapid clearance result in low absorption efficiency severely limit use local treatment. Methods: Herein, we present synthesis biodegradable polymeric Poly (D, L-Lactide-co-glycolide) (PLGA)...
Abstract DNA methylation is a fundamental epigenetic modification regulating gene expression. Aberrant the most common molecular lesion in cancer cells. However, medical intervention has been limited to use of broadly acting, small molecule-based demethylating drugs with significant side-effects and toxicities. To allow for targeted demethylation, we integrated two nucleic acid-based approaches: DNMT1 interacting RNA (DiR) aptamer strategy. By combining inherent capabilities inhibiting an...
Protein folding in the endoplasmic reticulum (ER) often involves formation of disulfide bonds. The oxidizing conditions required within this organelle were shown to be maintained through release small thiols, mainly cysteine and glutathione. Thiol secretion was stimulated when proteins rich bonds translocated into ER, prevented by inhibition protein synthesis. Endogenously generated glutathione counteracted thiol-mediated retention ER altered extracellular redox. thiols might link bond...
A synthetic ligand [TG19318], able to mimic protein in the recognition of immunoglobulin Fc portion, has been previously identified our laboratory through synthesis and screening multimeric combinatorial peptide libraries. In this study we have fully characterized its applicability affinity chromatography for downstream processing antibodies, examining specificity selectivity polyclonal monoclonal immunoglobulins derived from different sources. Ligand was broader than A, since IgG deriving...
Delayed neuronal cell death largely contributes to the progressive infarct development and associated functional impairments after cerebral ischemia or brain trauma. Previous studies exposed a key role for interaction of mitochondrial protein apoptosis-inducing factor (AIF) cytosolic cyclophilin A (CypA) in pathways programmed neurons vitro vivo. These suggested that pro-apoptotic activities AIF, such as its translocation nucleus subsequent DNA degradation, depend on physical AIF with CypA....
Abstract Precision medicine is continuously demanding for novel point of care systems, potentially exploitable also in-vivo analysis. Biosensing probes based on Lab-On-Fiber Technology have been recently developed to meet these challenges. However, devices exploiting standard label-free approaches (based ligand/target molecule interaction) suffer from low sensitivity in all cases where the detection small molecules at concentrations needed. Here we report a platform through combination with...
The integration of nanostructures able to manipulate light at the optical fiber tip is bringing fibers a renewed dimension since their beginnings. past decade has seen unprecedented advancements in lab-on-fiber technology field, pushed by effective exploitation nanoscale physics and supported improvement nanofabrication techniques. In this context, here we report on cavity enhanced optrode, which dramatically boosts performances widens range applications current systems, setting new...
Atlantic bonito meat has economic potential as an alternative to mackerel consumption. Thus, considering the presence of myoglobin (Mb) in red fish muscles, we report characterisation Mb compared and Tinker Mbs since this haemoprotein is implicated lipid oxidation preservation. A plethora biochemical approaches were employed purified from determine autoxidation rate constant (0.189±0.009 h−1), melting temperature (Tm= 72.84±1.02 °C) pseudoperoxidase activity different conditions (pH several...