A5083373234- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Immune Cell Function and Interaction
- Gut microbiota and health
- Viral-associated cancers and disorders
- Hepatitis B Virus Studies
- HIV Research and Treatment
- Immune Response and Inflammation
- Chromosomal and Genetic Variations
- Polyomavirus and related diseases
- Bacteriophages and microbial interactions
- HIV/AIDS drug development and treatment
- Cancer Research and Treatments
- SARS-CoV-2 and COVID-19 Research
Istituto Nazionale Tumori IRCCS "Fondazione G. Pascale"
2019-2024
Azienda Ospedaliera di Padova
2024
University of Padua
2024
Fondazione IRCCS Istituto Nazionale dei Tumori
2022
Istituti di Ricovero e Cura a Carattere Scientifico
2022
Background The host’s immune system develops in equilibrium with both cellular self-antigens and non-self-antigens derived from microorganisms which enter the body during lifetime. In addition, years, a tumor may arise presenting to an additional pool of non-self-antigens, namely antigens (tumor-associated antigens, TAAs; tumor-specific TSAs). Methods present study, we looked for homology between published TAAs non-self-viral-derived epitopes. Bioinformatics analyses ex vivo immunological...
Abstract Background The gut microbiota profile is unique for each individual and are composed by different bacteria species according to birth-to-infant transitions. In the last years, local systemic effects of on cancer onset, progression response treatments, such as immunotherapies, has been extensively described. Here we offer a new perspective, proposing role based molecular mimicry tumor associated antigens microbiome-associated antigens. Methods present study looked homology between...
We have recently shown extensive sequence and conformational homology between tumor-associated antigens (TAAs) derived from microorganisms (MoAs). The present study aimed to assess the breadth of T-cell recognition specific MoAs corresponding TAAs in healthy subjects (HS) patients with cancer (CP). A library > 100 peptide-MHC (pMHC) combinations was used generate DNA-barcode labelled multimers. Homologous peptides were selected Cancer Antigenic Peptide Database, as well...
Hepatocellular carcinoma (HCC) is the third leading cause of death from cancer globally. Indeed, only a few treatments are available, most which effective for early stages disease. Therefore, there an urgent needing potential markers specifically targeted therapy. Candidate proteins were selected datasets The Human Protein Atlas, in order to identify specific tumor-associated overexpressed HCC samples associated with poor prognosis. Potential epitopes predicted such proteins, and homology...
Abstract Background We have previously shown that HCC patients and healthy subjects are equally responsive to a RNAdjuvant ® , novel TLR-7/8/RIG-I agonist based on noncoding RNA developed by CureVac, an ex vivo evaluation. However, the immunological effect of adjuvants immune cells from cancer undergoing chemotherapy remains be demonstrated. Different currently used in vaccine clinical trials were evaluated present study before after setting. Methods PBMCs obtained 4 volunteers 23 affected...
Abstract The antigenicity as well the immunogenicity of tumor associated antigens (TAAs) may need to be potentiated in order break immunological tolerance. To this aim, heteroclitic peptides were designed introducing specific substitutions residue at position 4 (p4) binding TCR. effect such modifications also on affinity major histocompatibility class I (MHC-I) molecule was assessed. Trp2 antigen, for mouse melanoma B16F10 cells, HPV-E7 TC1 cell lines, used models. Affinity HLA predicted by...
Tumor Associated Antigens (TAAs) may suffer from an immunological tolerance due to expression on normal cells. In order potentiate their immunogenicity, heteroclitic peptides (htcPep) were designed according prediction algorithms. particular, specific modifications introduced in peptide residues facing TCR. Moreover, a MHC-optimized scaffold was for improved antigen presentation TCR by H-2Db allele. The efficacy of such htcPep assessed C57BL/6 mice injected with syngeneic melanoma B16F10 or...
Abstract Background People living with HIV/AIDS (PLWHA) show a reduced incidence for three cancer types, namely breast, prostate and colon cancers. In the present study, we assessed whether molecular mimicry between HIV epitopes tumor associated antigens and, consequently, T cell cross-reactivity could provide an explanation such epidemiological evidence. Methods Homology published TAAs non-self HIV-derived have been by BLAST homology. Structural analyses performed bioinformatics tools....
Abstract The host’s immune system may be primed against antigens during the lifetime (e.g. microorganisms antigens—MoAs), and swiftly recalled upon growth of a tumor expressing similar in sequence structure. C57BL/6 mice were immunized preventive setting with (TuAs) or corresponding heteroclitic peptides specific for TC-1 B16 cell lines. Immediately 2-months after end vaccination protocol, animals implanted anti-vaccine response as well regularly evaluated 2 months post-implantation. TuA...
Background In the present study we investigated whether peptides derived from entire SARS-CoV-2 proteome share homology to TAAs (tumor-associated antigens) and cross-reactive CD8+ T cell can be elicited by BNT162b2 preventive vaccine or natural infection. Methods results Viral epitopes with high affinity (<100nM) HLA-A*02:01 allele were predicted. Shared variant-specific identified. Significant homologies in amino acidic sequence have been found between multiple TAAs, mainly...
Abstract Background The gut microbiota profile is unique for each individual and are composed by different bacteria species according to birth-to-infant transitions. In the last years, local systemic effects of on cancer onset, progression response treatments, such as immunotherapies, has been extensively described. Here we offer a new perspective, proposing role based molecular mimicry tumor associated antigens microbiome-associated antigens. Methods present study looked homology between...
Background: People living with HIV /AIDS (PLWHA) show a reduced incidence for three cancer types, namely breast, prostate and colon cancers. In the present study, we assessed whether molecular mimicry between epitopes tumor associated antigens and, consequently, T cell cross-reactivity could provide an explanation such epidemiological evidence.Methods: Homology published TAAs non-self HIV-derived have been by BLAST homology. Structural analyses performed bioinformatics tools. Immunological...
Abstract Background: People living with HIV /AIDS (PLWHA) show a reduced incidence for three cancer types, namely breast, prostate and colon cancers. In the present study, we assessed whether molecular mimicry between epitopes tumor associated antigens and, consequently, T cell cross-reactivity could provide an explanation such epidemiological evidence. Methods: Homology published TAAs non-self HIV-derived have been by BLAST homology. Structural analyses performed bioinformatics tools....