A5029497920- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Melanoma and MAPK Pathways
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Complement system in diseases
- Cellular Mechanics and Interactions
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Chemical Synthesis and Analysis
- Angiogenesis and VEGF in Cancer
- Cellular transport and secretion
- Advanced Proteomics Techniques and Applications
- Computational Drug Discovery Methods
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Neuropeptides and Animal Physiology
- Hepatitis C virus research
- Biochemical and Molecular Research
- Alzheimer's disease research and treatments
- vaccines and immunoinformatics approaches
- Developmental Biology and Gene Regulation
Institute of Biostructure and Bioimaging
2017-2024
National Research Council
2019-2023
Centro Interuniversitario di Ricerca sui Peptidi Bioattivi
2019
University of Campania "Luigi Vanvitelli"
2017-2018
University of Naples Federico II
2010
Background The host’s immune system develops in equilibrium with both cellular self-antigens and non-self-antigens derived from microorganisms which enter the body during lifetime. In addition, years, a tumor may arise presenting to an additional pool of non-self-antigens, namely antigens (tumor-associated antigens, TAAs; tumor-specific TSAs). Methods present study, we looked for homology between published TAAs non-self-viral-derived epitopes. Bioinformatics analyses ex vivo immunological...
GADD45β is selectively and constitutively expressed in Multiple Myeloma cells, this expression correlates with an unfavourable clinical outcome. physically interacts the JNK kinase, MKK7, inhibiting its activity to enable survival of cancer cells. DTP3 a small peptide inhibitor GADD45β/MKK7 complex able restore MKK7/JNK activation, thereby promoting selective cell death GADD45β-overexpressing Enzymatic MS foot-printing diazirine-based chemical cross-linking (CX-MS) strategies were applied...
The transcription factor CCCTC-binding (CTCF) modulates pleiotropic functions mostly related to gene expression regulation. role of CTCF in large scale genome organization is also well established. A unifying model explain relationships among many CTCF-mediated activities involves direct or indirect interactions with numerous protein cofactors recruited specific binding sites. co-association other architectural proteins such as cohesin, chromodomain helicases, and BRG1, further supports the...
Abstract This study presents the synthesis of novel protected O‐glycosylated amino acid derivatives 1 and 2 , containing βGalNAc‐SerOBn βGalNAc‐ThrOBn units, respectively, as mimetics natural Tn antigen (αGalNAc‐Ser/Thr), along with solid‐phase assembly glycopeptides NHAcSer‐Ala‐Pro‐Asp‐Thr[αGalNAc]‐Arg‐Pro‐Ala‐Pro‐Gly‐BSA ( 3 ‐BSA) NHAcSer‐Ala‐Pro‐Asp‐Thr[βGalNAc]‐Arg‐Pro‐Ala‐Pro‐Gly‐BSA 4 ‐BSA), bearing αGalNAc‐Thr or βGalNAc‐Thr MUC1 tumor mucin glycoproteins. According to ELISA tests,...
Bicyclic peptides assembled around small organic scaffolds are gaining an increasing interest as new potent, stable and highly selective therapeutics because of their uncommon ability to specifically recognize protein targets, size that favor tissue penetration the versatility easiness synthesis. We have here rationally designed bicyclic a common tri-bromo-methylbenzene moiety in order mimic structure CFC domain oncogene Cripto-1 and, more specifically, orient most fruitful way hot spot...
GADD45β/MKK7 complex is a non-redundant, cancer cell-restricted survival module downstream of the NF-kB pathway, and it has pathogenically critical role in multiple myeloma, an incurable malignancy plasma cells. The first-in-class inhibitor DTP3 effectively kills MM cells expressing its molecular target, both vitro vivo, by inducing MKK7/JNK-dependent apoptosis with no apparent toxicity to normal combines favorable drug-like properties, on-target-specific pharmacology, resulting safe...
Oral squamous cell carcinoma (OSCC) is a common epithelial malignancy of the oral cavity. Nodal and Cripto-1 (CR-1) are important developmental morphogens expressed in several adult cancers associated with disease progression. Whether CR-1 simultaneously same tumor how this affects cancer biology unclear. We investigate expression potential role both human OSCC. Immunohistochemistry results show that OSCC sample intensity staining correlated advanced-stage disease. However, was not observed...
Age-related macular degeneration (AMD) is the primary cause of blindness in advanced countries. Repeated intravitreal delivery anti-vascular endothelial growth factor (VEGF) agents has represented an important advancement for therapy wet AMD with significative results terms prevention and partial vision restore. Nonetheless, some patients are not responsive or do attain significant visual improvement, injection may serious complications side effects have been reported prolonged block VEGF-A....
Background: Monoclonal antibodies (mAbs) against cancer biomarkers are key reagents in diagnosis and therapy. One such relevant biomarker is a preferentially expressed antigen melanoma (PRAME) that selectively many tumors. Knowing mAb’s epitope of utmost importance for understanding the potential activity therapeutic prospective reagents. Methods: We generated mAb PRAME immunizing mice with fragment 161–415; affinity antibody protein was evaluated by ELISA SPR, its ability to detect cells...
The three members (GADD45α, GADD45β, and GADD45γ) of the growth arrest DNA damage-inducible 45 (GADD45) protein family are involved in a myriad diversified cellular functions. With aim unravelling analogies differences, we performed comparative biochemical biophysical analyses on proteins. characterization quantification their binding to MKK7 kinase, validated functional partner indicate that GADD45α GADD45γ strong interactors kinase. Despite remarkable sequence similarity, proteins present...
CD59 is an abundant immuno-regulatory human protein that protects cells from damage by inhibiting the complement system. inhibits assembly of Membrane Attack Complex (MAC), bactericidal pore-forming toxin innate immune In addition, several pathogenic viruses, including HIV-1, escape complement-mediated virolysis incorporating this inhibitor in their own viral envelope. This makes such as not neutralised fluids. also overexpressed cancer to resist attack. Consistent with its importance a...
Drug development in recent years is increasingly focused on developing personalized treatments based blocking molecules selective for therapeutic targets specifically present individual patients. In this perspective, the specificity of and agents plays a crucial role. Monoclonal antibodies (mAbs) their surrogates are used context thanks to ability bind inhibit activity or transport bioactive into compartments which expressed. Small antibody-like molecules, such as Fabs, often certain...
APEH is a ubiquitous and cytosolic serine protease belonging to the prolyl oligopeptidase (POP) family, playing critical role in processes of degradation proteins through both exo- endopeptidase events. Endopeptidase activity has been associated with protein oxidation; however, actual mechanisms have yet be elucidated. We show that synthetic fragment GDF11 spanning region 48-64 acquires sensitivity only when methionines are transformed into corresponding sulphoxide derivatives. The data...