A5095888289- Inflammatory mediators and NSAID effects
- Biochemical and Structural Characterization
- Calcium signaling and nucleotide metabolism
- NF-κB Signaling Pathways
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Natural product bioactivities and synthesis
- Monoclonal and Polyclonal Antibodies Research
- Complement system in diseases
- RNA Research and Splicing
- Chemical Synthesis and Analysis
Istituto Pasteur
2024
Sapienza University of Rome
2021-2024
The glucosamine derivative 2-(N-Acetyl)-L-phenylalanylamido-2-deoxy-β-D-glucose (NAPA), was shown to inhibit the kinase activity of IKKα, one two catalytic subunits IKK complex, decreasing inflammatory status in osteoarthritis chondrocytes. In present work we have investigated inhibition mechanism IKKα by NAPA combining computational simulations, vitro assays and Mass Spectrometry (MS) technique. assay conducted using a recombinant IKKtide, 20 amino acid peptide substrate derived from IkBα...
Abstract Inteins are proteins involved in the protein splicing mechanism, an autoprocessing event, where sequences (exteins) separated by inteins become ligated each other after recombination. Two kinds of have been described, contiguous and split inteins. The former ones transcribed translated as a single peptide along with their exteins, while latter fragmented between two different genes separately. aim this study is to establish method obtain fluorescent eukaryotic analyze its cellular...